The Interface of Cancer, Their Microenvironment and Nanotechnology

Roque, Natália R.; Matias, Diana; Bal鏰-Silva, Joana; Ferrer, Val閞ia Pereira; Pessôa, Luciana Fontes; Spohr, Tânia Cristina Leite de Sampaio e

doi:10.32604/oncologie.2022.024035

Cited by 3 publications

(1 citation statement)

References 260 publications

(290 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Nevertheless, the recent development of hypoxia-responsive nanoparticles that selectively release their cargo under hypoxic conditions in the TME [323,324] provides a perspective for the specific delivery of the UPR branches inhibitors including 4µ8C for IRE1 [325,326], ISRIB for PERK [327], or Ceapins for ATF6 [328]. This approach would provide an excellent mechanism for the selective analysis of these pathway functions in the context of the hypoxic TME.…”

Section: Discussionmentioning

confidence: 99%

The Role of the Hypoxia-Related Unfolded Protein Response (UPR) in the Tumor Microenvironment

Bartoszewska

Collawn

Bartoszewski

2022

Cancers

View full text Add to dashboard Cite

Despite our understanding of the unfolded protein response (UPR) pathways, the crosstalk between the UPR and the complex signaling networks that different cancers utilize for cell survival remains to be, in most cases, a difficult research barrier. A major problem is the constant variability of different cancer types and the different stages of cancer as well as the complexity of the tumor microenvironments (TME). This complexity often leads to apparently contradictory results. Furthermore, the majority of the studies that have been conducted have utilized two-dimensional in vitro cultures of cancer cells that were exposed to continuous hypoxia, and this approach may not mimic the dynamic and cyclic conditions that are found in solid tumors. Here, we discuss the role of intermittent hypoxia, one of inducers of the UPR in the cellular component of TME, and the way in which intermittent hypoxia induces high levels of reactive oxygen species, the activation of the UPR, and the way in which cancer cells modulate the UPR to aid in their survival. Although the past decade has resulted in defining the complex, novel non-coding RNA-based regulatory networks that modulate the means by which hypoxia influences the UPR, we are now just to beginning to understand some of the connections between hypoxia, the UPR, and the TME.

show abstract

Section: Discussionmentioning

confidence: 99%

The Role of the Hypoxia-Related Unfolded Protein Response (UPR) in the Tumor Microenvironment

Bartoszewska

Collawn

Bartoszewski

2022

Cancers

View full text Add to dashboard Cite

show abstract

Using single‐cell RNA sequencing and bulk RNA sequencing data to reveal a correlation between smoking and neutrophil activation in esophageal carcinoma patients

Ba,

2024

Environmental Toxicology

View full text Add to dashboard Cite

BackgroundCigarette smoking is considered as a major risk factor for esophageal carcinoma (ESCA) patients. Neutrophil activation plays a key role in cancer development and progression. However, the relationship between cigarette smoking and neutrophils in ESCA patients remained unclear.MethodsSingle‐cell RNA sequencing (scRNA‐seq) and bulk RNA sequencing data were obtained from public databases. Uniform manifold approximation and projection (UMAP) was used to perform downscaling and clustering based on scRNA‐seq data. The module genes associated with smoking in ESCA patients were filtered by weighted gene co‐expression network analysis (WGCNA). Using the “AUCell” package, the enrichment of different cell subpopulations and gene collections were assessed. “CellChat” and “CellphoneDB” were used to infer the probability and significance of ligand–receptor interactions between different cell subpopulations.ResultsWGCNA was performed to screened module genes associated with smoking in ESCA patients from MEdarkquosie, MEturquoise, and MEgreenyellow. Next, eight cell clusters were identified, and using the AUCell score, we determined that neutrophil clusters were more active in the gene modules associated with smoking in ESCA patients. Two neutrophil subtypes, Neutrophils 1 and Neutrophils 2, exhibited greater enrichment in inflammatory response regulation, intercellular adhesion, and regulation of T cell activation. Furthermore, we found that neutrophils may pass through AMPT‐(ITGA5 + ITGB1) and ICAM1‐AREG in order to promote the development of ESCA, and that the expression levels of the receptor genes insulin‐degrading enzyme and ITGB1 were significantly and positively correlated with cigarette smoking per day.ConclusionCombining smoking‐related gene modules and scRNA‐seq, the current findings revealed the heterogeneity of neutrophils in ESCA and a tumor‐promoting role of neutrophils in the tumor microenvironment of smoking ESCA patients.

show abstract

Single cell RNA sequencing – a valuable tool for cancer immunotherapy: a mini review

Kodous,

Balaiah,

Ramanathan

2023

Oncologie

View full text Add to dashboard Cite

Single-cell RNA sequencing (scRNA-seq) technology has made great strides in research over the last decade. Data analysis has been aided by developments in bioinformatics tools and artificial intelligence, allowing biological and clinical researchers to get a deeper understanding of the different cell clusters and their dynamics within tumours. Combining conventional treatment modalities like chemotherapy and radiation with immunotherapy is a growing trend in cancer treatment. Hence, knowledge of the tumour microenvironment and the effect of each treatment modality on the TME, at a single cell level can provide treating clinicians with better clues for patient stratification and prognostication. With this knowledge, immunotherapy could become successful in treating a wide range of cancers, opening the path for the creation of even more effective treatment strategies. Despite the widespread availability of scRNA-seq technology, computational analysis and data interpretation are still challenges. Worldwide, such challenges are being addressed by various researchers, strengthening the contribution of this technology towards cancer elimination. In this mini-review, we primarily focus on the technique, its workflow, and the computational aspects of scRNA technology, along with an overview of the current challenges in the analysis and interpretation of the data generated.

show abstract

The Interface of Cancer, Their Microenvironment and Nanotechnology

Cited by 3 publications

References 260 publications

The Role of the Hypoxia-Related Unfolded Protein Response (UPR) in the Tumor Microenvironment

The Role of the Hypoxia-Related Unfolded Protein Response (UPR) in the Tumor Microenvironment

Using single‐cell RNA sequencing and bulk RNA sequencing data to reveal a correlation between smoking and neutrophil activation in esophageal carcinoma patients

Single cell RNA sequencing – a valuable tool for cancer immunotherapy: a mini review

Contact Info

Product

Resources

About