The interactome of the UapA transporter reveals putative new players in anterograde membrane cargo trafficking

Georgiou, Xenia; Dimou, Sofia; Diallinas, George; Samiotaki, Martina

doi:10.1016/j.fgb.2023.103840

Cited by 2 publications

References 85 publications

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A type II phosphatidylinositol-4-kinase coordinates sorting of cargo polarizing by endocytic recycling

Kourkoulou,

Martzoukou,

Fischer

et al. 2024

Commun Biol

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Depending on their phosphorylation status, derivatives of phosphatidylinositol play important roles in vesicle identity, recognition and intracellular trafficking processes. In eukaryotic cells, phosphatidylinositol-4 phosphate pools generated by specific kinases are key determinants of the conventional secretion pathways. Earlier work in yeast has classified phosphatidylinositol-4 kinases in two types, Stt4p and Pik1p belonging to type III and Lsb6p to type II, with distinct cellular localizations and functions. Eurotiomycetes appear to lack Pik1p homologues. In Aspergillus nidulans, unlike homologues in other fungi, AnLsb6 is associated to late Golgi membranes and when heterologously overexpressed, it compensates for the thermosensitive phenotype in a Saccharomyces cerevisiae pik1 mutant, whereas its depletion leads to disorganization of Golgi-associated PHOSBP-labelled membranes, that tend to aggregate dependent on functional Rab5 GTPases. Evidence provided herein, indicates that the single type II phosphatidylinositol-4 kinase AnLsb6 is the main contributor for decorating secretory vesicles with relevant phosphatidylinositol-phosphate species, which navigate essential cargoes following the route of apical polarization via endocytic recycling.

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A type II phosphatidylinositol-4-kinase coordinates sorting of cargo polarizing by endocytic recycling

Kourkoulou,

Martzoukou,

Fischer

et al. 2024

Commun Biol

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Neosynthesized polarized and non-polarized plasma membrane cargoes follow distinct trafficking routes inAspergillus nidulans

Sagia,

Georgiou,

Chamilos

et al. 2024

Preprint

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Membrane proteins are thought to be sorted to the plasma membrane (PM) via Golgi-dependent trafficking. However, our recent studies in the fungusAspergillus nidulanschallenged the essentiality of Golgi in the biogenesis of non-polarly localized transporters and receptors. Here, we investigate the mechanism of trafficking of membrane proteins, by following the localization of a polar cargo (R-SNARE SynA) versus a non-polar cargo (UapA transporter), synchronously co-expressed in wild-type or isogenic genetic backgrounds repressible for conventional cargo secretion. In wild-type, the two cargoes dynamically label distinct secretory compartments, highlighted by the observation that, unlike SynA, UapA does not colocalize with the late-Golgi. In line with partitioning into distinct early secretory carriers, UapA and SynA translocation to the PM is differentially dependent on Sec13, and importantly the two cargoes collapse in distinct early secretory compartments in a sec31ts mutant or upon CopA repression. Trafficking via distinct cargo-specific carriers is further supported by the observation that repression or inactivation of key proteins essential for late-Golgi /TGN maturation and post-Golgi vesicular secretion did not affect proper trafficking of UapA, but totally blocked SynA secretion. Surprisingly, several specific SNARE proteins that are absolutely essential for conventional cargo vesicular secretion, as well as the exocyst effector RabDSec4, proved dispensable for UapA translocation to the PM. Our findings point to a model where UapA proper trafficking and insertion into the PM might involve non-canonical SNARE combinations. Overall, the present work establishes unequivocally the existence of distinct, cargo-dependent, trafficking mechanisms, initiating at early secretory compartments.

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The interactome of the UapA transporter reveals putative new players in anterograde membrane cargo trafficking

Cited by 2 publications

References 85 publications

A type II phosphatidylinositol-4-kinase coordinates sorting of cargo polarizing by endocytic recycling

A type II phosphatidylinositol-4-kinase coordinates sorting of cargo polarizing by endocytic recycling

Neosynthesized polarized and non-polarized plasma membrane cargoes follow distinct trafficking routes inAspergillus nidulans

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