The Interactions of Some Cations with Deoxyribonucleic Acid Observed by the Use of Toluidine Blue as an Indicator

Huse, Yukiko; Miyaki, Komei; Tsuboi, Masamichi

doi:10.1246/bcsj.38.1039

Cited by 7 publications

(2 citation statements)

References 5 publications

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“…T Ahe ability of nucleosides (Edelman et a!., 1960;Eichhorn et al, 1966; Ropars and Viovy, 1965a), nucleotides (Eichhorn and Clark, 1965;Rich, 1959), and nucleic acids (Trim, 1959; Yamane and Davidson, 1962;Fuse et al, 1965;Coates et al, 1965) to complex with certain metals has been reported by various investigators. Occurrence of some metals in nucleic…”

mentioning

confidence: 95%

The Interaction of Silver Ion with Guanosine, Guanosine Monophosphate, and Related Compounds. Determination of Possible Sites of Complexing^*

Tu¹,

Reinosa²

1966

Biochemistry

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nize" both residues forming the bond. Moreover, when different peptides containing the same terminal bonds are compared, large differences in reactivity are observed. Thus, with LAP, Ala, (ldll) is not digested under our conditions, Ala3 (ldl) is digested in trace amounts only, and Ala, (lddl) is digested at an appreciable rate. Yet all three peptides contain the N-terminal LD-type bond. Additional examples of this kind can be found in Table III. This is an indication that not only the nature of the bond hydrolyzed determines its susceptibility to cleavage, but that residues further removed also exhibit an influence on the rate of enzymatic attack. Similar findings with respect to the action of CP-A on ll bonds have been described in a preliminary communication . It was shown by comparing kinetic rate constants that the enzyme "recognizes" at least four residues in the substrate. This was deduced from the observation that the C-terminal bonds of the three tetrapeptides llll, dlll, and ldll are split at relative rates of about 100:10:1. Other proteolytic enzymes seem to behave in a similar manner. Papain was shown to interact simultaneously with five residues in the substrate . Results obtained re-cently in this laboratory indicate that trypsin and aspergillopeptidase B (Subramanian and Kalnitsky, 1964) recognize a sequence of five and seven residues, respectively, in a peptide chain.

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confidence: 95%

The Interaction of Silver Ion with Guanosine, Guanosine Monophosphate, and Related Compounds. Determination of Possible Sites of Complexing^*

Tu¹,

Reinosa²

1966

Biochemistry

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“…This is considered to reflect the broadening of metachromatic band to longer wavelength region over the monomer band observed in absorption spectra of TB with DNA in solution. 12 Wang et al studied CD spectra of TB solutions with addition of different amounts of calf thymus DNA. 28 They suggested multiple binding modes of TB to DNA affected by their concentration ratio.…”

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confidence: 99%

Consideration on the Metachromatic Spectra of Toluidine Blue Dimers Formed on DNA Oligomers

Sato¹,

Matsumoto

Freivalds³

et al. 2010

Bulletin of the Chemical Society of Japan

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Metachromatic staining with toluidine blue (TB) has been used as a cytological tool for tissue recognition and cancer diagnosis. Recently, its strong potential for infertility diagnosis was also reported. Metachromatic staining is important in biological applications, but the origin of spectral changes has not been fully understood, although dye aggregation on biological materials is thought to be the cause. In this study, we investigated the dimer structure of TB formed on DNA oligomers by using a computational method, particularly focusing on the spectral changes caused by TB dimer formation. The structure of the TBDNA complexes was constructed on the basis of the calculated molecular structure of TB and crystal data of A-and B-form DNA oligomers, assuming that there was an electrostatic interaction between them. The resulting spectral shift was then evaluated using the extended-dipole model. The examination of B-DNA revealed that possible TB dimers result in a hypsochromic spectral shift of absorption. On the other hand, the dimerization of TB on A-DNA was found to be quite difficult, because the helical geometry of A-DNA restricted the binding sites of TB. These results suggest that the metachromatic color observed during biological staining is significantly affected by the helical geometry of DNA.

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Polyelectrolyte complexes, 1. The effect of pH and ionic strength on the stoichiometry of model polycation—polyanion complexes

et al. 1979

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The interactions between model polycations and polyanions have been studied using the fluorescent dye acridine orange as a probe. The dye emission at 540 nm is quenched by the addition of polyanions, a process which is reversed by the addition of certain polycations. Techniques have been developed which enable a determination of the stoichiometry of polyelectrolyte complexes so formed between three polyanions (carboxymethylcellulose, K-carrageenan and poly(styrenesu1fonate)) and two polycations [poly(L-lysine hydrobromide) and poly(p-xylylviologen dibromide)) . A one to one pairing of acidic sites with basic groups was observed over a wide range of temperature, pH and ionic strength. Deviations from unit stoichiometry in the presence of multi-charged metal ions have been interpreted as due to conformational changes in the polyanion. Results from viscosity measurements are included which suggest the neutralisation of charged groups in the polyelectrolyte complex.

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The Interactions of Some Cations with Deoxyribonucleic Acid Observed by the Use of Toluidine Blue as an Indicator

Cited by 7 publications

References 5 publications

The Interaction of Silver Ion with Guanosine, Guanosine Monophosphate, and Related Compounds. Determination of Possible Sites of Complexing^*

The Interaction of Silver Ion with Guanosine, Guanosine Monophosphate, and Related Compounds. Determination of Possible Sites of Complexing^*

Consideration on the Metachromatic Spectra of Toluidine Blue Dimers Formed on DNA Oligomers

Polyelectrolyte complexes, 1. The effect of pH and ionic strength on the stoichiometry of model polycation—polyanion complexes

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The Interactions of Some Cations with Deoxyribonucleic Acid Observed by the Use of Toluidine Blue as an Indicator

Cited by 7 publications

References 5 publications

The Interaction of Silver Ion with Guanosine, Guanosine Monophosphate, and Related Compounds. Determination of Possible Sites of Complexing*

The Interaction of Silver Ion with Guanosine, Guanosine Monophosphate, and Related Compounds. Determination of Possible Sites of Complexing*

Consideration on the Metachromatic Spectra of Toluidine Blue Dimers Formed on DNA Oligomers

Polyelectrolyte complexes, 1. The effect of pH and ionic strength on the stoichiometry of model polycation—polyanion complexes

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The Interaction of Silver Ion with Guanosine, Guanosine Monophosphate, and Related Compounds. Determination of Possible Sites of Complexing^*

The Interaction of Silver Ion with Guanosine, Guanosine Monophosphate, and Related Compounds. Determination of Possible Sites of Complexing^*