The interactions between nucleic acids and polyamines

Aikens, D. A.; Bunge, Stanley; Onasch, Frederick; Parker, Raymond; Hurwitz, Charles; Clemans, S. D.

doi:10.1016/0301-4622(83)87015-x

Cited by 60 publications

(16 citation statements)

References 15 publications

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“…[151]) by 13 C NMR [155], for spermine (10) and spermidine (11) by 1 H [156] and 13 C NMR [153,154], for thermospermine by 15 N NMR [157] and for the neuroactive wasp toxin philanthotoxin-343 by 1 H and 13 C NMR titration [158]. Microconstants have been determined by NMR titration for N-(2-mercaptoethyl)-1,3-diaminopropane [159], tetren [27], spermine, spermidine, and homologues [154,160].…”

Section: Open-chain Polyaminesmentioning

confidence: 99%

Determination of microscopic acid?base parameters from NMR?pH titrations

Szakács

Kraszni

Noszál

2004

Analytical and Bioanalytical Chemistry

106

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The theory and practice of proton microspeciation based on NMR-pH titrations are surveyed. Principles of bi-, tri-, tetra-, and n-protic microequilibrium systems are discussed. Evaluation methods are exemplified by case studies on bi- and tetraprotic biomolecules. Selection criteria and properties of 'reporter' NMR nuclei are described. Literature data on complete microspeciations of small ligands and site-specific basicity characterizations of peptides and proteins are critically reviewed.

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Section: Open-chain Polyaminesmentioning

confidence: 99%

Determination of microscopic acid?base parameters from NMR?pH titrations

Szakács

Kraszni

Noszál

2004

Analytical and Bioanalytical Chemistry

106

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“…Among these polyamines, spermine is reported to possess the highest biological activity 10 . Under physiologic ionic and pH conditions, polyamines are multivalent cations with aliphatic hydrocarbon chains separating the charges 11 , and hence negatively charged nucleic acids, including DNA and RNA, are their prime targets of interaction, and consequently regulate the structures of DNA, condense DNA molecules to a liquid crystalline phase 12 , and enhance the activity of G-quadruplex/hemin DNAzymes in vitro 13 . Many evidences suggest that polyamines play important roles as modulators of nucleic acid structure 14 .…”

Section: Introductionmentioning

confidence: 99%

Oxidative degradation of polyamines by serum supplement causes cytotoxicity on cultured cells

Wang

Liu

et al. 2018

Sci Rep

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Serum is a common supplement for cell culture due to it containing the essential active components for the growth and maintenance of cells. However, the knowledges of the active components in serum are incomplete. Apart from the direct influence of serum components on cultured cells, the reaction of serum components with tested drugs cannot be ignored, which usually results in the false conclusion on the activity of the tested drugs. Here we report the toxicity effect of polyamines (spermidine and spermine) on cultured cells, especially on drug-resistant cancer cell lines, which resulted from the oxidative degradation of polyamines by amine oxidases in serum supplement. Upon adding spermidine or spermine, high concentration of H2O2, an enzyme oxidation product of polyamines, was generated in culture media containing ruminant serum, such as fetal bovine serum (FBS), calf serum, bovine serum, goat serum or horse serum, but not in the media containing human serum. Drug-resistant cancer cell lines showed much higher sensitivity to the oxidation products of polyamines (H2O2 and acrolein) than their wild cell lines, which was due to their low antioxidative capacity.

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“…Taking these works together, it appears likely that the predominant conformations of polyamines depend upon a balance between stabilizing intramolecular H-bonds and minimization of repulsive steric and electrostatic interactions and that these are strongly influenced by the nature and pH of the medium ( 2 ). Titration studies monitoring pKa's of spermine and spermidine suggest at physiological pH all polyamine nitrogens are probably mainly in the protonated state, which is likely significant not only for their conformation but also their properties in cells ( 6 , 7 ).…”

Section: Introductionmentioning

confidence: 99%

Endogenous polyamine function—the RNA perspective

Lightfoot

Hall

2014

Nucleic Acids Research

116

107

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Recent progress with techniques for monitoring RNA structure in cells such as ‘DMS-Seq’ and ‘Structure-Seq’ suggests that a new era of RNA structure-function exploration is on the horizon. This will also include systematic investigation of the factors required for the structural integrity of RNA. In this context, much evidence accumulated over 50 years suggests that polyamines play important roles as modulators of RNA structure. Here, we summarize and discuss recent literature relating to the roles of these small endogenous molecules in RNA function. We have included studies directed at understanding the binding interactions of polyamines with polynucleotides, tRNA, rRNA, mRNA and ribozymes using chemical, biochemical and spectroscopic tools. In brief, polyamines bind RNA in a sequence-selective fashion and induce changes in RNA structure in context-dependent manners. In some cases the functional consequences of these interactions have been observed in cells. Most notably, polyamine-mediated effects on RNA are frequently distinct from those of divalent cations (i.e. Mg2+) confirming their roles as independent molecular entities which help drive RNA-mediated processes.

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The interactions between nucleic acids and polyamines

Cited by 60 publications

References 15 publications

Determination of microscopic acid?base parameters from NMR?pH titrations

Determination of microscopic acid?base parameters from NMR?pH titrations

Oxidative degradation of polyamines by serum supplement causes cytotoxicity on cultured cells

Endogenous polyamine function—the RNA perspective

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