The interaction of transfer ribonucleic acid with 50 S ribosomal subunit proteins

The interaction of 5 S RNA and its large fragments with ribosomal proteins

“…Under the experimental conditions, in agreement with [ 13,25,26], the immobilized intact 5 S RNA forms a complex with 3 ribosoma1 proteins: L5, L18 and L25. An identical result was obtained with the immobilized larger and smaller fragment, provided they were complemented with the free smaller or larger fragment, respectively (table 1).…”

The domain for transfer ribonucleic acid binding to the Escherichia coli ribosome

“…Various control experiments were detailed [3,4]. Besides those, one more criterion for specificity became evident from the fact that proteins bound to the immobilized tRNA can be washed out from the gel with a binding buffer solution containing excess tR.NA (data not shown).…”

“…In sum, spontaneous assembly of the tRNAprotein complex occurs when free ribosomal proteins are passed through a tRNA-Sepharose affinity column. This complex contains almost all the 30 S subunit proteins [3], identified at the ribosomal decoding site as well as 50 S subunit proteins [4] identified in or near to the peptidyl transferase center. Because of cooperativity observed between 30 S and 50 S subunit proteins during assembly (see above), we suggest that the complex represents an analogue of the subunits interface and is a compact, structurally integrated domain of ribosome, where both tRNA-protein and protein-protein interactions may be involved (discussed [4]).…”

“…All experimental procedures used in this work were as in [3,4]. The columns containing 0.3-0.5 ml RNAgel (corresponds to l-2 mg tRNA) were equilibrated with Tris-HCl buffer, pH 7.5,6 mM 2-mercaptoethanol and various concentrations of MgC12 and potassium chloride.…”

“…It was shown that a set of ribosomal proteins containing both 30 S subunit and 50 S subunit proteins, interact with tRNA immobilized to Sepharose [ 1,2] . Also, separately from each other, a number of both 30 S and 50 S subunit proteins were found to bind to immobilized tRNA [3,4] . In this paper we show that there is cooperativity between 30 S and 50 S subunit proteins in the assembly of tRNA-protein complex.…”