The interaction of styrene maleic acid copolymers with phospholipids in Langmuir monolayers, vesicles and nanodiscs; a structural study

Hall, Stephen B.; Tognoloni, Cecilia; Campbell, Richard A.; Richens, Joanna L.; O’Shea, Paul; Terry, Ann E.; Price, Gareth J.; Dafforn, Timothy R.; Edler, Karen J.; Arnold, Thomas

doi:10.1016/j.jcis.2022.03.102

Cited by 6 publications

(1 citation statement)

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“…After solubilization, remove the insoluble membrane particles by low-speed (~3000 rpm) centrifugation for 30 min to 1 h. At this stage, the membrane lipids are solubilized by the polymer, and polymer-lipid-membrane protein complexes are formed ( Figure 1 ). Similar to MSP, SMA co-polymers, when mixed with cell membrane/synthetic lipids, form membrane mimicking flat-bilayered disc-like structures called polymer–lipid nanoparticles/nanodiscs ( Figure 6 ) [ 121 , 122 , 123 , 124 , 125 ]. The target membrane protein in soluble local lipid-nanodiscs (in the supernatant) is then purified by either affinity chromatography [ 110 ] or ion-exchange chromatography.…”

Section: Detergent-free Isolation Of Membrane Proteins Using Amphipat...mentioning

confidence: 99%

Detergent-Free Isolation of Membrane Proteins and Strategies to Study Them in a Near-Native Membrane Environment

Krishnarjuna

Ramamoorthy

2022

Biomolecules

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Atomic-resolution structural studies of membrane-associated proteins and peptides in a membrane environment are important to fully understand their biological function and the roles played by them in the pathology of many diseases. However, the complexity of the cell membrane has severely limited the application of commonly used biophysical and biochemical techniques. Recent advancements in NMR spectroscopy and cryoEM approaches and the development of novel membrane mimetics have overcome some of the major challenges in this area. For example, the development of a variety of lipid-nanodiscs has enabled stable reconstitution and structural and functional studies of membrane proteins. In particular, the ability of synthetic amphipathic polymers to isolate membrane proteins directly from the cell membrane, along with the associated membrane components such as lipids, without the use of a detergent, has opened new avenues to study the structure and function of membrane proteins using a variety of biophysical and biological approaches. This review article is focused on covering the various polymers and approaches developed and their applications for the functional reconstitution and structural investigation of membrane proteins. The unique advantages and limitations of the use of synthetic polymers are also discussed.

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