The Interaction of Sheep Genomic DNA with a Cobalt(II) Complex Containing p-Nitrobenzoate and N,N`-Diethylnicotinamide Ligands

Abstract

Abstract:The synthesized cobalt(II) complex, CoPNBDENA and the binding of this complex with sheep genomic DNA were investigated by UV-Visible absorption and viscosity techniques. Also the interaction of sheep genomic DNA with the complex was studied using the agarose gel electrophoresis method. The results indicated that the complex interacted with DNA. The nature of the binding seemed to be mainly an electrostatic interaction between DNA and the cobalt(II) complex. Other binding modes such as hydrogen bonds may also exist in this system. In this study, after the interaction of DNA-CoPNBDENA, it was observed that the migration of the DNA band became slow as the amount of cobalt(II) complex was increased. This clearly demonstrates that the CoPNBDENA complex neutralizes the negative charges of DNA.

DNA Binding and Cleavage Activities of Na[B(Glu)(OH)2]·2H2O, Na[B(Cit)(OH)2]·2H2O, Li[B(Sal)(OH)2], and Mg[B(Sal)(OH)2]·2H2O Complexes

“…All the solvents and the reagents were obtained from Merck, Turkey. Genomic DNA was extracted from calf through the earlier reported methods [26]. Peripheral blood samples (6-9 mL) of calves were collected in EDTA tubes and DNA samples were isolated by Promega Wizard genomic DNA purification kit (Cat.…”

Recent Updates of DNA Incorporated in Carbon Nanotubes and Nanoparticles for Electrochemical Sensors and Biosensors

“…The CNT purification has also been accomplished by stirring the CNT in concentrated nitric acid at 25 °C for 24 h [68]. Other than the above wet oxidation methods, numerous acid treatments are also available in the literature, which have been used for the purification of CNTs [45, 69-77]. Other purification methods have also been reported in the literature such as, the electrochemical oxidation of MWCNTs in NaCl or NaBr solution, which leads to halogenation of MWCNTs and the formation of oxygen containing functional groups on MWCNTs [78]; the etching of individual MWCNT in a KCl solution by applying a positive potential higher enough for the occurrence of the electrochemical oxidation [79]; purification by a galvanostatic electrochemical oxidation in H 2 SO 4 .…”

“…These nanoscale systems demonstrate unique catalytic or recognition properties [39]. The immobilization of these proteins on nanostructured materials such as CNTs with colloidal gold hybrids, montmorillonite, clay, mesoporous materials and molecular sieves have been identified as very promising methods for biosensing applications [40-45]. In the literature, electrochemical sensor device designing, selection of suitable sensing material for suitable analyte and their wide applications are already abundant [46-49].…”

Thermal properties of solid complexes with biologically important heterocyclic ligands

“…In order to enhance understanding of biologically important ligand-metal ion interactions, we have been studying the thermal and spectral properties of halogenobenzoato copper(II) complexes with N,N-diethylnicotinamide. This reveal the relationship between the structure and thermolysis of dimeric carboxylate complexes of the type Cu 2 (n-Xbz) 4 (denia) 2 (n = 2 or 3, X = Cl or Br). This work is a continuation of our previously studies [6][7][8][9][10][11][12] on the thermal properties of copper(II) and magnesium(II) complexes with biologically important heterocyclic ligands.…”

“…N,N-diethylnicotinamide (denia) is toxic agent, but it is used as respiratory stimulant [4,5]. It exhibits different biological activities when coordinated to central atom depending also on the central atom nature.…”