The interaction of MYB, bHLH and WD40 transcription factors in red pear (Pyrus pyrifolia) peel

Cui, Dao‐Lei; Shu-xin, Zhao; Xu, Huini; Allan, Andrew C.; Zhang, Xiaodong; Fan, Lei; Chen, Limei; Su, Jun; Qin, Shu; Li, Kunzhi

doi:10.1007/s11103-021-01160-w

Cited by 33 publications

(15 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Eight TTG1 proteins from different plants were found to be 78.2%–99.12% homologous, indicating that the functions of TTG1s are conserved in different species. In the present study, FcWD40-97 showed the highest similarity to TTG1s of other plants, with a deduced amino acid sequence that had 79.88% similarity to AtTTG1 ( Li et al, 2014 ), 88.01 and 87.94% to apple TTG1 and peach WD40, respectively ( An et al, 2012 ; Cui et al, 2021 ), 85.67, 85.17, and 85.59% to CsWD40, FaTTG1, and Punica granatum (Pg) TTG1, respectively ( Ben-Simhon et al, 2011 ; Schaart et al, 2013 ; Liu et al, 2018 ), and 82.90 and 80.52% to G ossypium hirsutum (Gh) TTG3 and Medicago truncatula (Mt) WD40-1 ( Humphries et al, 2005 ; Pang et al, 2009 ), respectively. Moreover, FcWD40-97 shared the four highly conserved WDR motifs with other TTG1s, reported to be related to anthocyanin biosynthesis.…”

Section: Discussionmentioning

confidence: 48%

“…FcTTG1 encoded 339 amino acids, which is more than grape TTG1 (336), but less than TTG1 of Arabidopsis (341), strawberry (344) and Camellia sinensis (342). In addition, FcTTG1 has 5 WDR motifs, similar to strawberry ( Schaart et al, 2013 ), tea tree ( Liu et al, 2018 ), grape ( Jiu et al, 2021 ) and pear ( Cui et al, 2021 ), whereas Arabidopsis ( Baudry et al, 2004 ), Chinese bayberry ( Liu et al, 2013 ) and pomegranate ( Ben-Simhon et al, 2011 ) each have 4 WDR motifs.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Genome-Wide Analysis of Anthocyanin Biosynthesis Regulatory WD40 Gene FcTTG1 and Related Family in Ficus carica L.

et al. 2022

View full text Add to dashboard Cite

WD40 proteins serve as crucial regulators in a broad spectrum of plant developmental and physiological processes, including anthocyanin biosynthesis. However, in fig (Ficus carica L.), neither the WD40 family nor any member involved in anthocyanin biosynthesis has been elucidated. In the present study, 204 WD40 genes were identified from the fig genome and phylogenetically classified into 5 clusters and 12 subfamilies. Bioinformatics analysis prediction localized 109, 69, and 26 FcWD40 proteins to the cytoplasm, nucleus and other cellular compartments, respectively. RNA-seq data mining revealed 127 FcWD40s expressed at FPKM > 10 in fig fruit. Most of these genes demonstrated higher expression in the early stages of fruit development. FcWD40-97 was recruited according to three criteria: high expression in fig fruit, predicted nuclear localization, and closest clustering with TTG1s identified in other plants. FcWD40-97, encoding 339 amino acids including 5 WD-repeat motifs, showed 88.01 and 87.94% amino acid sequence similarity to apple and peach TTG1, respectively. The gene is located on fig chromosome 4, and is composed of 1 intron and 2 exons. Promoter analysis revealed multiple light-responsive elements, one salicylic acid-responsive element, three methyl jasmonate-responsive elements, and one MYB-binding site involved in flavonoid biosynthesis gene regulation. FcWD40-97 was in the FPKM > 100 expression level group in fig fruit, and higher expression was consistently found in the peel compared to the flesh at the same development stages. Expression level did not change significantly under light deprivation, whereas in leaves and roots, its expression was relatively low. Transient expression verified FcWD40-97’s localization to the nucleus. Yeast two-hybrid (Y2H) and biomolecular fluorescence complementation (BiFC) assays revealed that FcWD40-97 interacts with FcMYB114, FcMYB123, and FcbHLH42 proteins in vitro and in vivo, showing that FcWD40-97 functions as a member of the MYB–bHLH–WD40 (MBW) complex in anthocyanin-biosynthesis regulation in fig. We therefore renamed FcWD40-97 as FcTTG1. Our results provide the first systematic analysis of the FcWD40 family and identification of FcTTG1 in fig pigmentation.

show abstract

Section: Discussionmentioning

confidence: 48%

Section: Discussionmentioning

confidence: 99%

Genome-Wide Analysis of Anthocyanin Biosynthesis Regulatory WD40 Gene FcTTG1 and Related Family in Ficus carica L.

et al. 2022

View full text Add to dashboard Cite

show abstract

“…TMO5/LHW activity is known to be repressed by the SACL bHLH proteins, which compete with TMO5 for binding to LHW and thus reduce the amount of active TMO5/LHW dimers (Katayama et al, 2015; Vera-Sirera et al, 2015). Given the well documented interactions between MYB and bHLH TFs (Zhao et al, 2008; Carretero-Paulet et al, 2010; Feller et al, 2011; Cui et al, 2021), we hypothesized that MYB12 function might involve direct binding to the TMO5/LHW complex, similar to the SACL mode-of-action (Katayama et al, 2015; Vera-Sirera et al, 2015). Firstly, if MYB12 were to bind the TMO5/LHW complex, it would need to be present in the same cells.…”

Section: Resultsmentioning

confidence: 99%

“…Besides forming bHLH homo-or heterodimers, bHLH proteins have also been shown to directly interact with other proteins such as MYB-type TFs, which can enhance or supress their transcriptional activity (Zhao et al, 2008; Carretero-Paulet et al, 2010; Feller et al, 2011; Cui et al, 2021). MYB TFs are defined by their highly conserved DNA-binding MYB-domain that contains up to four α-helical “R” repeats (Ogata et al, 1996; Du et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

MYB12 spatiotemporally represses TMO5/LHW-mediated transcription in the Arabidopsis root meristem

Wybouw

Arents

Yang

et al. 2022

Preprint

View full text Add to dashboard Cite

Transcriptional networks are crucial to integrate various internal and external signals into optimal responses during plant growth and development. Primary root vasculature patterning and proliferation are controlled by a network centred around the basic Helix-Loop-Helix transcription factor complex formed by TARGET OF MONOPTEROS 5 (TMO5) and LONESOME HIGHWAY (LHW), which control cell proliferation and orientation by modulating cytokinin response and other downstream factors. Despite recent progress, many aspects of the TMO5/LHW pathway are not fully understood. In particular, the upstream regulators of TMO5/LHW activity remain unknown. Here, using a forward genetic approach to identify new factors of the TMO5/LHW pathway, we discovered a novel function of the MYB-type transcription factor MYB12. MYB12 physically interacts with TMO5 and dampens the TMO5/LHW-mediated induction of direct target gene expression as well as the periclinal/radial cell divisions. The expression of MYB12 is activated by the cytokinin response, downstream of TMO5/LHW, resulting in a novel MYB12-mediated negative feedback loop that restricts TMO5/LHW activity to ensure optimal cell proliferation rates during root vascular development.

show abstract

“…Most anthocyanin biosynthesis genes are regulated by the MBW transcription factor complex comprising MYB, bHLH, and WD40 [ 26 , 27 ]. Transcription factors can activate structural-gene expression.…”

Section: Introductionmentioning

confidence: 99%

Anthocyanin regulatory networks in Solanum tuberosum L. leaves elucidated via integrated metabolomics, transcriptomics, and StAN1 overexpression

et al. 2022

View full text Add to dashboard Cite

Background Anthocyanins, which account for color variation and remove reactive oxygen species, are widely synthesized in plant tissues and organs. Using targeted metabolomics and nanopore full-length transcriptomics, including differential gene expression analysis, we aimed to reveal potato leaf anthocyanin biosynthetic pathways in different colored potato varieties. Results Metabolomics analysis revealed 17 anthocyanins. Their levels varied significantly between the different colored varieties, explaining the leaf color differences. The leaves of the Purple Rose2 (PurpleR2) variety contained more petunidin 3-O-glucoside and malvidin 3-O-glucoside than the leaves of other varieties, whereas leaves of Red Rose3 (RedR3) contained more pelargonidin 3-O-glucoside than the leaves of other varieties. In total, 114 genes with significantly different expression were identified in the leaves of the three potato varieties. These included structural anthocyanin synthesis–regulating genes such as F3H, CHS, CHI, DFR, and anthocyanidin synthase and transcription factors belonging to multiple families such as C3H, MYB, ERF, NAC, bHLH, and WRKY. We selected an MYB family transcription factor to construct overexpression tobacco plants; overexpression of this factor promoted anthocyanin accumulation, turning the leaves purple and increasing their malvidin 3-o-glucoside and petunidin 3-o-glucoside content. Conclusions This study elucidates the effects of anthocyanin-related metabolites on potato leaves and identifies anthocyanin metabolic network candidate genes.

show abstract

The interaction of MYB, bHLH and WD40 transcription factors in red pear (Pyrus pyrifolia) peel

Cited by 33 publications

References 43 publications

Genome-Wide Analysis of Anthocyanin Biosynthesis Regulatory WD40 Gene FcTTG1 and Related Family in Ficus carica L.

Genome-Wide Analysis of Anthocyanin Biosynthesis Regulatory WD40 Gene FcTTG1 and Related Family in Ficus carica L.

MYB12 spatiotemporally represses TMO5/LHW-mediated transcription in the Arabidopsis root meristem

Anthocyanin regulatory networks in Solanum tuberosum L. leaves elucidated via integrated metabolomics, transcriptomics, and StAN1 overexpression

Contact Info

Product

Resources

About