The Interaction of Mammalian Interferons with Immobilized Cibacron Blue F3Ga: Modulation of Binding Strength

Abstract

Mouse, hamster, rabbit, horse, and human interferons bind to immobilized Cibacron Blue F3GA under appropriate solvent conditions. Three forms of the immobilized ligand have been investigated: Cibacron Blue F3GA-Sepharose 4B, Blue Dextran-Sepharose 4B and Blue Sepharose CL-6B. The strength of binding of an interferon depends critically on the sorbent: Cibacron Blue F3GA-Sepharose 4B is the weakest in the series and Blue Sepharose CL-6B the strongest. The use of Blue Dextran-Sepharose 4B--a sorbent of intermediate binding properties--allows the complete separation of hamster, mouse and human fibroblast interferons in a single chromatographic step. Indeed, both the resolution, as well as the recovery, of those interferons is complete--regardless of the relative complexity of the chromatographed preparation (containing either crude or purified interferons). Thus, these ligands should prove of considerable use when a facile chromatographic evaluation, both qualitative and quantitative of mammalian interferons is required.

Species-specific posttranscriptional regulation of interferon synthesis

“…Incubation for 15 min at room temperature abolished the ability of the translation product to protect GM-258 cells against VSV challenge. (3) Chromatography of the translation product on blue dextran-Sepharose columns was done in accordance with the procedure of Jankowski et al (1976) and Bollin et al (1978). Blue dextran-Sepharose, a sulfonated polyaromatic blue dye covalently linked to Sepharose, was chosen for chromatographic characterization since human fibroblast IF binds very strongly to this ligand by means of both ionic and hydrophobic interactions.…”

Rapid analytical and preparative affinity chromatography using resin‐bound cibacron blue F3G‐A

“…Campylobacter species [2,3]. It was suspected that these differences could also be reflected in their lipid patterns.…”

Cibacron Blue F3GA ile modifiye polimerik mikroküreler ile RuBisCO adsorpsiyonu

“…Daha sonra bu yaklaşım, CB immobilize edilmiş farklı adsorbanların kullanılması ile çok sayıda proteinin saflaştırılmasında kullanılmıştır [15,16,17]. Birçok boya ligandının, özellikle de CB ve analoglarının bazı oksidoredüktazlar, fosfokinazlar ve ATPazlar ile etkileşimleri araştırılmıştır [18,19]. Elde edilen sonuçlar, boya yapısındaki antrakinon ve benzen sülfonat halkalarının enzimlere bağlanmada önemli olduğunu göstermiştir.…”