The interaction of lipopolysaccharide with membrane receptors on macrophages pretreated with extract of Reishi polysaccharides measured by optical tweezers

Wei, Ming-Tzo; Hua, Kuo-Feng; Hsu, Jowey; Karmenyan, Artashes; Tseng, Kai-Yu; Wong, Chi‐Huey; Hsu, Han‐Shui; Chiou, Arthur

doi:10.1364/oe.15.011020

Cited by 20 publications

(7 citation statements)

References 14 publications

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“…Recently, Wei et al (2007) found that GLPS not only enhances the J774A.1 macrophage surface expression of TLR4 and CD14 as well as LPS binding and phagocytosis internalization, but it also reduces the adhesion time constant and increases the force constant of the binding interaction. Treatment with F3, an active ingredient of GLPS, increased the population of CD14 þ CD26 þ monocyte/macrophage in human umbilical cord blood mononuclear cells (Chien et al, 2004).…”

Section: Effect Of Glps On Macrophagesmentioning

confidence: 99%

Ganoderma lucidum Polysaccharides: Immunomodulation and Potential Anti-Tumor Activities

Chen²,

Zhong³

et al. 2011

Am. J. Chin. Med.

273

167

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Ganoderma lucidum (G. lucidum), a basidiomycete white rot fungus, has long been prescribed to prevent and treat various human diseases, particularly in China, Japan, and Korea. Several classes of bioactive substances have been isolated and identified from G. lucidum, such as triterpenoids, polysaccharides, nucleosides, sterols, and alkaloids, among others. This paper examines the potential role of G. lucidum polysaccharide (GLPS) in tumor therapy and the possible mechanisms involved. Both in vitro and in vivo studies suggested that the anti-tumor activities of GLPS are mediated by its immunomodulatory, anti-angiogenic, and cytotoxic effects. GLPS affects immune cells and immune-related cells including B lymphocytes, T lymphocytes, dendritic cells, macrophages, and natural killer cells. In addition, recent data also suggest that GLPS suppresses tumorigenesis or inhibits tumor growth through direct cytotoxic effect and anti-angiogenic actions. However, many questions still need to be answered before both G. lucidum and GLPS can be widely accepted and used as anti-tumor agents.

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Section: Effect Of Glps On Macrophagesmentioning

confidence: 99%

Ganoderma lucidum Polysaccharides: Immunomodulation and Potential Anti-Tumor Activities

Chen²,

Zhong³

et al. 2011

Am. J. Chin. Med.

273

167

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“…To demonstrate that an interaction does exist between the bacterium and the substratum, the interaction force (represented by a force constant K bio ) between a single bacterium and the surrounding agar gel (0.2%) in LB was measured by oscillatory optical tweezers (Figure 3); similar techniques have been applied successfully to measure the interaction between biomolecules and mammalian cells [14], [15]. The mean value of K bio for strain BW25113 was 34.2±7.0 dyne/cm.…”

Section: Resultsmentioning

confidence: 99%

“…To measure the interaction of the bacterium with the surrounding LB agar gel in terms of the interaction force constant K bio , a previously described method [15] was modified. In brief, when bacteria grew to an appropriate density, the bacterial solution was dropped into LB-agar to make a final agar concentration at 0.2% (w/v).…”

Section: Methodsmentioning

confidence: 99%

“…where “K OT ” , the spring constant of the optical trap, was pre-determined to be 9.9±0.3 pN/µm [32], [33]; “A”, the amplitude of the oscillatory trapping beam was 32 nm; “D”, the amplitude of the bacterium oscillation, and “δ”, the relative phase of bacterium oscillation relative to that of the beam focus, were measured by a quadrant photo diode in conjunction with a lock-in amplifier [15], [34].…”

Section: Methodsmentioning

confidence: 99%

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Factors Affecting Daughter Cells' Arrangement during the Early Bacterial Divisions

Yen²,

Wang

et al. 2010

PLoS ONE

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On agar plates, daughter cells of Escherichia coli mutually slide and align side-by-side in parallel during the first round of binary fission. This phenomenon has been previously attributed to an elastic material that restricts apparently separated bacteria from being in string. We hypothesize that the interaction between bacteria and the underneath substratum may affect the arrangement of the daughter bacteria. To test this hypothesis, bacterial division on hyaluronic acid (HA) gel, as an alternative substratum, was examined. Consistent with our proposition, the HA gel differs from agar by suppressing the typical side-by-side alignments to a rare population. Examination of bacterial surface molecules that may contribute to the daughter cells' arrangement yielded an observation that, with disrupted lpp, the E. coli daughter cells increasingly formed non-typical patterns, i.e. neither sliding side-by-side in parallel nor forming elongated strings. Therefore, our results suggest strongly that the early cell patterning is affected by multiple interaction factors. With oscillatory optical tweezers, we further demonstrated that the interaction force decreased in bacteria without Lpp, a result substantiating our notion that the side-by-side sliding phenomenon directly reflects the strength of in-situ interaction between bacteria and substratum.

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“…The cell culture chamber containing an objective heater (Bioptechs) is mounted on an inverted microscope (Olympus IX81). Combining optical tweezers with known optical spring constants as force sensors and phase-sensitive detection 24,[45][46][47] , we measure the viscoelasticity in living cells. Notably, since the cytoplasm has different optical properties from that of the medium, the optical spring constant used for measuring intracellular stiffness modulus would be corrected via the refractive index mismatching 24 .…”

Section: Methodsmentioning

confidence: 99%

Intracellular nonequilibrium fluctuating stresses indicate how nonlinear cellular mechanical properties adapt to microenvironmental rigidity

Wei

Jedlicka

Ou-Yang

2020

Sci Rep

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Living cells are known to be in thermodynamically nonequilibrium, which is largely brought about by intracellular molecular motors. The motors consume chemical energies to generate stresses and reorganize the cytoskeleton for the cell to move and divide. However, since there has been a lack of direct measurements characterizing intracellular stresses, questions remained unanswered on the intricacies of how cells use such stresses to regulate their internal mechanical integrity in different microenvironments. This report describes a new experimental approach by which we reveal an environmental rigidity-dependent intracellular stiffness that increases with intracellular stress-a revelation obtained, surprisingly, from a correlation between the fluctuations in cellular stiffness and that of intracellular stresses. More surprisingly, by varying two distinct parameters, environmental rigidity and motor protein activities, we observe that the stiffness-stress relationship follows the same curve. This finding provides some insight into the intricacies by suggesting that cells can regulate their responses to their mechanical microenvironment by adjusting their intracellular stress.

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The interaction of lipopolysaccharide with membrane receptors on macrophages pretreated with extract of Reishi polysaccharides measured by optical tweezers

Cited by 20 publications

References 14 publications

Ganoderma lucidum Polysaccharides: Immunomodulation and Potential Anti-Tumor Activities

Ganoderma lucidum Polysaccharides: Immunomodulation and Potential Anti-Tumor Activities

Factors Affecting Daughter Cells' Arrangement during the Early Bacterial Divisions

Intracellular nonequilibrium fluctuating stresses indicate how nonlinear cellular mechanical properties adapt to microenvironmental rigidity

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