The Interaction of Human Serum Albumin with Long-chain Fatty Acid Anions

Goodman, DeWitt S.

doi:10.1021/ja01548a024

Cited by 728 publications

(230 citation statements)

References 7 publications

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“…1 is that both at pH 6.4 and at pH 9.2 the AZ3-versus-rF relationship can be split up into three parts at about rF = 2 and rF = 7. It is striking that this classification is very similar to the classification given by Goodman (1958), namely two high-affinity sites, five medium-affinity sites and about 20 low-affinity sites. C.d.…”

Section: Results and Discussion Ph-stat Experimentssupporting

confidence: 79%

“…The existence of these conformational changes was deduced from experiments which are described by step 2 in Scheme 1; the experiments discussed here, however, are based on step 3 in Scheme 1. The present results also suggest that the course of these N-N* and B-B* transitions is determined by the classification of the oleic acid-binding sites proposed by Goodman (1958).…”

Section: Results and Discussion Ph-stat Experimentssupporting

confidence: 73%

“…1 shows the relation between AZ3 and the molar ratio of oleic acid to albumin (rF) at pH 6.4 and 9.2. By using the binding parameters for the binding of oleic acid to albumin calculated by Goodman (1958), it can be demonstrated that, between rF = 0 and rF = 9, the value of rF is approximately equal, within 10 %, to the number of oleic acid molecules bound to one albumin molecule (VF). This means that Fig.…”

Section: Results and Discussion Ph-stat Experimentsmentioning

confidence: 99%

“…Since Ashbrook et al (1975) proved that the binding of oleic acid to albumin does not have a co-operative character, the latter assumption is probably more correct. With help of the data for the interaction of oleic acid with albumin obtained by Goodman (1958), we calculated v1, V2 and v3 for every rF value. Because of the rF independence of, [6] at vE > 9, a value of 2-was chosen for V3.…”

Section: Results and Discussion Ph-stat Experimentsmentioning

confidence: 99%

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The fatty-acid-induced conformational states of human serum albumin investigated by means of multiple co-binding of protons and oleic acid

Dröge

Janssen

Wilting

1988

Biochemical Journal

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The binding of oleic acid to human serum albumin causes progressive changes in (a) the pK of some amino acid residues, as detected by pH-stat titration and (b) the induced molar ellipticities of albumin-bound drugs (diazepam and oxyphenbutazone), as measured by c.d. It is concluded that albumin undergoes several conformational transitions as the amount of oleic acid bound increases from 0 to about 9 molecules/ molecule of protein. At least three different conformations of the protein seem to be involved. These conformations can be linked with the three classes of oleic acid-binding sites on albumin.

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Section: Results and Discussion Ph-stat Experimentssupporting

confidence: 79%

Section: Results and Discussion Ph-stat Experimentssupporting

confidence: 73%

Section: Results and Discussion Ph-stat Experimentsmentioning

confidence: 99%

Section: Results and Discussion Ph-stat Experimentsmentioning

confidence: 99%

See 2 more Smart Citations

The fatty-acid-induced conformational states of human serum albumin investigated by means of multiple co-binding of protons and oleic acid

Dröge

Janssen

Wilting

1988

Biochemical Journal

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“…4 g/dl) is approximately 20 times that of GFP (0.2 g/dl, provided as a component of the elution buffer used in gel filtering the platelets). It is well known that albumin binds fatty acid anions with high affinity and that the concentration of unbound fatty acid anion in aqueous solution is a function of the fatty acid to albumin molar ratio (24,25). As shown in Fig.…”

Section: Discussionmentioning

confidence: 98%

Release of platelet-derived growth factor from human platelets by arachidonic acid

Linder

Chernoff

Kaplan

et al. 1979

Proc. Natl. Acad. Sci. U.S.A.

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Platelet a-granules contain a factor that stimulates the proliferation of arterial smooth muscle cells and may play a role in atherogenesis. We have studied the role of arachidonic acid in mediating the release of the platelet-derived growth factor (PDGF) from human platelets. PDGF was assayed by stimulation of [3Hlthymidine incororation into DNA of mouse 3T3 cells. Platelet aggregation and the release of platelet factor 4, P-thromboglobulin, and serotonin were also studied.A biphasic response pattern was observed when gel-filtered platelets were incubated with arachidonate over the concentration range 0.01-0.4 mM. At low arachidonate levels (approximately 0.025-0.1 mM), specific concentration-dependent aggregation and release of PDGF and of the other components were observed. This effect was not seen with any of five other fatty acids tested and was suppressed by indomethacin (25 uM). At higher arachidonate concentrations (approximately 0.15-0.35 mM), a concentration-dependent turn-off of both aggregation and release occurred. At these concentrations the platelets remained functional, and no release of lactate dehydrogenase was observed. A similar biphasic pattern of arachidonate-induced aggregation and release was observed with platelet-rich plasma, over a similar range of arachidonate to albumin mole ratios. These studies demonstrate that PDGF and other a-granule constituents can be released from platelets specifically by arachidonate via an indomethacin-sensitive pathway, most probably involving the platelet cyclooxygenase and conversion of arachidonate to prostaglandin metabolites. The mechanisms responsible for the turn-off of the specific arachidonate-mediated responses at higher arachidonate concentrations remain to be defined.

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The Significance of Nonesterified Fatty Acids in Plasma

Dole¹

1958

Arch Intern Med

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The Interaction of Human Serum Albumin with Long-chain Fatty Acid Anions

Cited by 728 publications

References 7 publications

The fatty-acid-induced conformational states of human serum albumin investigated by means of multiple co-binding of protons and oleic acid

The fatty-acid-induced conformational states of human serum albumin investigated by means of multiple co-binding of protons and oleic acid

Release of platelet-derived growth factor from human platelets by arachidonic acid

The Significance of Nonesterified Fatty Acids in Plasma

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