The interaction of glycosides and saccharides with antibody to the corresponding phenylazo glycosides

Yariv, J.; Rapport, Maurice M.; Graf, Liselotte

doi:10.1042/bj0850383

Cited by 295 publications

(174 citation statements)

References 7 publications

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“…This electrophoresis was carried out following the protocol described by Ding and Zhu (1997) using an agarose gel (1 %) 1 mm thickness (25 mM Tris, 200 mM glycine, pH8.4) containing 2 mg/mL of Yariv β-glucosyl (Yariv β-glucosyl is a red stain from the combination of α-aminophényl), β-D-nitrogen glycosides in hydrochloric acid and phloroglucinol (Yariv et al 1962). A range of arabic gum AG (0; 0.125; 0.2; 0.5; 1 mg/mL) served as standard.…”

Section: Rocket Electrophoresis Of Taro Agpsmentioning

confidence: 99%

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Mucilage chemical profile and antioxidant properties of giant swamp taro tubers

et al. 2012

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Section: Rocket Electrophoresis Of Taro Agpsmentioning

confidence: 99%

“…The presence of arabinose and galactose associated to the relatively high percentage of protein strongly suggested that this mucilage contained an AGP. Indeed an AGP consists of a hydroxyproline-rich core protein which is decorated by arabinose and galactose-rich polysaccharide units (Yariv et al 1962). …”

Section: Characteristics Of Isolated Gst Mucilagementioning

confidence: 99%

Mucilage chemical profile and antioxidant properties of giant swamp taro tubers

et al. 2012

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“…The AGP artificial antigen ~-glucosyl Yariv reagent (1,3,5-Tris [4-~-o-glucopyranosyl-oxyphenylazo]-2,4,6-trihydroxybenzene;Nothnagel and Lyon, 1986;Yariv et aL, 1962) was from Biosupplies Australia Ltd (Parkville, Australia). The anti-AGP mAbs were from immunizations with different kinds of plant cell preparations and extracts (Bradley et aL, 1988;Dewey et aL, 1997;Knox et al, 1989;Norman etaL, 1986;Pennell etal., 1989).…”

Section: Plants and Molecular Probesmentioning

confidence: 99%

Oxidative cross‐linking of plasma membrane arabinogalactan proteins

et al. 1997

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SummaryMonoclonal antibodies which recognize carbohydrate in arabinogalactan proteins (AGPs) have revealed that certain carbohydrate epitopes at the outer plasma membrane surface are developmentally regulated. Some epitopes are expressed according to cell position, and AGPs are thought to play a role in cell-cell interaction during development. This study demonstrates that sugar beet plasma membranes contain two subfamilies of AGPs, with apparent molecular masses of 82 and 97 kDa, and that each subfamily consists of a small number of acidic AGP isoforms. Excision of leaves generates three additional AGP complexes with apparent molecular masses of 120, 170 and 210 kDa, with the 170 kDa complex being the major form induced by excision. The addition of millimolar concentrations of H202 to a partially purified fraction of the 82 and 97 kDa AGPs also generates AGP complexes, with the 170 kDa complex as the major form. These results indicate that the plasma membrane AGPs are a target for endogenous H202.

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“…To assess total AGP secretion in 3-d-old control and auxin-deprived cells, rocket-gel electrophoresis with Yariv's artificial antigen (Yariv et al, 1962) was used. Auxin-deprived cells secreted twice as much AGP as control cells, as judged by the height of the rockets (Fig.…”

Section: Analysis Of Agpsmentioning

confidence: 99%

Auxin Deprivation Induces Synchronous Golgi Differentiation in Suspension-Cultured Tobacco BY-2 Cells1

Winicur

Zhang²,

Staehelin

1998

Plant Physiology

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To date, the lack of a method for inducing plant cells and their Golgi stacks to differentiate in a synchronous manner has made it difficult to characterize the nature and extent of Golgi retailoring in biochemical terms. Here we report that auxin deprivation can be used to induce a uniform population of suspension-cultured tobacco (Nicotiana tabacum cv BY-2) cells to differentiate synchronously during a 4-d period. Upon removal of auxin, the cells stop dividing, undergo elongation, and differentiate in a manner that mimics the formation of slime-secreting epidermal and peripheral root-cap cells. The morphological changes to the Golgi apparatus include a proportional increase in the number of trans-Golgi cisternae, a switch to larger-sized secretory vesicles that bud from the trans-Golgi cisternae, and an increase in osmium staining of the secretory products. Biochemical alterations include an increase in large, fucosylated, mucin-type glycoproteins, changes in the types of secreted arabinogalactan proteins, and an increase in the amounts and types of molecules containing the peripheral root-capcell-specific epitope JIM 13. Taken together, these findings support the hypothesis that auxin deprivation can be used to induce tobacco BY-2 cells to differentiate synchronously into mucilage-secreting cells.

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The interaction of glycosides and saccharides with antibody to the corresponding phenylazo glycosides

Cited by 295 publications

References 7 publications

Mucilage chemical profile and antioxidant properties of giant swamp taro tubers

Mucilage chemical profile and antioxidant properties of giant swamp taro tubers

Oxidative cross‐linking of plasma membrane arabinogalactan proteins

Auxin Deprivation Induces Synchronous Golgi Differentiation in Suspension-Cultured Tobacco BY-2 Cells1

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