The interaction of deoxyribonucleic acid and acridine orange

Boyle, Robert E.; Nelson, Sol S.; Dollish, Francis R.; Olsen, Marvin J.

doi:10.1016/0003-9861(62)90448-4

Cited by 34 publications

(4 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The agents were added as 5 ,l or less from stocks in 50% ethanol, and the mixtures were incubated for 1 hr at room temperature in the dark. Changes in the emission spectra of the dyes indicative of intercalation were observed (21,22) with a Hitachi-Perkin Elmer MPF-2A fluorescence spectrophotometer. When E. coli DNA was incubated with ICR-191 in 25 mM triethanolamine * HCl, (pH 7.20) at 65°for 10 min, then subjected to denaturation by heat or formamide (17), the ICR-191 fluorescence became nondialyzable, indicating covalent attachment of the compound to DNA by monofunctional alkylation.…”

Section: Methodsmentioning

confidence: 99%

Uncoupling of the recBC ATPase from DNase by DNA Crosslinked with Psoralen

Karu

Linn

1972

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

Exonucleolytic cleavage of DNA by the recBC DNase is accompanied by a DNA-dependent ATP hydrolysis that ceases when the DNA has been digested to a limit. On the other hand, DNA that has been crosslinked by 4,5',8-trimethylpsoralen in the presence of 360-nm light remains an effective cofactor in the ATPase reaction, but is resistant to digestion by the enzyme. Psoralentreated DNA is degraded by pancreatic DNase, micrococcal nuclease, and Escherichia coli B restriction enzyme, but not by Neurospora crassa nuclease, suggesting that crosslinking did not grossly distort the duplex structure of the DNA. The psoralen-DNA is not a potent inhibitor, but competes with single-stranded DNA from bacteriophage fd for the recBC DNase to roughly the same extent as does normal duplex DNA. DNA treated with psoralen in the dark, exposed to 360-nm light in the absence of psoralen, or treated with the intercalating agents ethidium bromide, 9-aminoacridine, ICR-191, or actinomycin D, responds to the enzyme no differently from untreated DNA. However, DNA crosslinked with mitomycin C or nitrogen mustard behaves similarly to psoralen-treated DNA. The relationship of these findings to models for the function and control of the recBC ATPase and nuclease, and the advantages of psoralen as a DNA crosslinking agent, are discussed.The recB and recC genes of Escherichia coli control a deoxyribonuclease assumed to function in DNA recombination and rapair. This enzyme degrades duplex DNA exonucleolytically to short oligonucleotides and also catalyzes a DNA-dependent hydrolysis of ATP to ADP and Pi (1, 2). Furthermore, ATP-dependent DNases from other microorganisms have been reported that possess DNA-dependent ATPase activity (3-6). The ATPase reaction of these enzymes is curious in that up to 40 ATP molecules can be hydrolyzed per DNA phosphodiester bond broken, with the exact ratio depending upon the reaction conditions (3-7). In addition, there is no theoretical energy requirement for catalysis of the exothermic DNase reaction.To clarify the relation of ATP hydrolysis to the DNase reaction, we have been studying the action of the recBC nuclease on DNA substrates with well-defined localized perturbations or structural alterations. Cole has demonstrated that introduction of crosslinks into DNA with psoralen is considerably more lethal for E. coli mutants defective in repair or recombination than it is for the wild-type strains (8). Also, crosslinked DNA is an inefficient substrate in vitro for phage A exonuclease, the exonuclease activity of DNA polymerase I, Abbreviations: Psoralen-DNA, DNA that has been crosslinked by irradiation at 360 nm in the presence of psoralen (4,5'-8-trimethyl[furano-3',2':6,7-coumarin]); recBC ATPase or recBC DNase, the DNA-dependent ATPase and the DNase activities catalyzed by the enzyme controlled by the reeB and recC genes of E. coli. The DNase activity has also been referred to as exonuclease V (2). and other enzymes likely to be involved in recombination and repair (9). These findings prompted us to obse...

show abstract

Section: Methodsmentioning

confidence: 99%

Uncoupling of the recBC ATPase from DNase by DNA Crosslinked with Psoralen

Karu

Linn

1972

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

show abstract

“…Boyle et af. (254) further studied the interaction of DNA and acridine orange. Weill and Calvin (255) investigated the optical properties of proflavine and acridine orange upon being bound to polyphosphates and DNA.…”

Section: Noncov Alently Bound Labelsmentioning

confidence: 99%

Fluorescent Protein Conjugates

Dandliker

Portmann

1971

Excited States of Proteins and Nucleic Acids

View full text Add to dashboard Cite

“…Unfortunately it is difficult at the moment to relate these measurements to the fine structure of the complex; often the appropriate calculations are intractable. Additional difficulties which the histophysicist might expect in this field are illustrated by the fluorescence quenching measurements which Boyle et al (1962) have carried out. They found that acridine orange interacts with DNA (and other polyanions ' 2) anomalously in contradistinction to corisphosphine and other aminoacridine dyes which do not.…”

Section: O N C L U S I O Nmentioning

confidence: 99%

Studies in fluorescence histochemistry: I. The demonstration of sulphomucins*

STOWARD

1967

Journal of the Royal Microscopical Society

View full text Add to dashboard Cite

SYNOPSISSulphated mucosubstances in sections of coagulant‐fixed tissue emit a variable yellow to red fluorescence after being stained with purified coriphosphine at pH 2 to 4. Unfortunately this fluorescence is sometimes indistinguishable from a similarly coloured fluorescence emitted by nuclei, oxidized sulphoproteins and some sialo‐mucins, which, however, can be eliminated more or less selectively either by (1) a preliminary Feulgen hydrolysis and condensation with N,N‐dimethyl‐m‐phenylene diamine; or (2) by pretreatment with a solution of ferric alum; or (3) by a previous methylation with methanolic thionyl chloride, followed by reduction with lithium aluminium hydride in hot dioxane and then by saponification (a sequence of reactions which, in theory but not always in practice, should remove all polyanionic groups in the tissue except sulphate groups on sulphated mucopolysaccharides); or (4), best of all, by staining sections in a very dilute solution of thiazol yellow at pH 2 after they have been stained in coriphosphine.The rationale and limitations of these methods are discussed critically.

show abstract

The interaction of deoxyribonucleic acid and acridine orange

Cited by 34 publications

References 13 publications

Uncoupling of the recBC ATPase from DNase by DNA Crosslinked with Psoralen

Uncoupling of the recBC ATPase from DNase by DNA Crosslinked with Psoralen

Fluorescent Protein Conjugates

Studies in fluorescence histochemistry: I. The demonstration of sulphomucins*

Contact Info

Product

Resources

About