The interaction between Pseudomonas aeruginosa cells and cationic PC:Chol:DOTAP liposomal vesicles versus outer-membrane structure and envelope properties of bacterial cell

Drulis-Kawa, Zuzanna; Dorotkiewicz-Jach, Agata; Gubernator, Jerzy; Guła, Grzegorz; Bocer, Tomasz; Doroszkiewicz, W.

doi:10.1016/j.ijpharm.2008.09.043

Cited by 57 publications

(45 citation statements)

References 36 publications

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“…The outer membrane proteins, lipopolysaccharides (LPS), hydrophobicity and electrostatic potential of the bacterial cells were taken into account. [39] Using PC:Chol:DOTAP liposomes containing the fluorophore rhodamine B, no influence of outer membrane proteins involved in the efflux pump system on the fusion was found. Likewise, the LPS did not have a significant impact on the interactions with the liposomes since strains exposing the same kind of LPS showed a different uptake of liposomal rhodamine B.…”

Section: Fusogenic Liposomesmentioning

confidence: 96%

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Lipid and polymer nanoparticles for drug delivery to bacterial biofilms

Forier

Raemdonck

Smedt

et al. 2014

Journal of Controlled Release

356

320

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Biofilms are matrix-enclosed communities of bacteria that show increased antibiotic resistance and the capability to evade the immune system. They can cause recalcitrant infections which cannot be cured with classical antibiotic therapy. Drug delivery by lipid or polymer nanoparticles is considered a promising strategy for overcoming biofilm resistance. These particles are able to improve the delivery of antibiotics to the bacterial cells, thereby increasing the efficacy of the treatment. In this review we give an overview of the types of polymer and lipid nanoparticles that have been developed for this purpose. The antimicrobial activity of nanoparticle encapsulated antibiotics compared to the activity of the free antibiotic is discussed in detail. In addition, targeting and triggered drug release strategies to further improve the antimicrobial activity are reviewed. Finally, ample attention is given to advanced microscopy methods that shed light on the behavior of nanoparticles inside biofilms, allowing further optimization of the nanoformulations. Lipid and polymer nanoparticles were found to increase the antimicrobial efficacy in many cases. Strategies such as the use of fusogenic liposomes, targeting of the nanoparticles and triggered release of the antimicrobial agent ensured the delivery of the antimicrobial agent in close proximity of the bacterial cells, maximizing the exposure of the biofilm to the antimicrobial agent. The majority of the discussed papers still present data on the in vitro anti-biofilm activity of nanoformulations, indicating that there is an urgent need for more in vivo studies in this field.

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Section: Fusogenic Liposomesmentioning

confidence: 96%

“…An 18 kDa outer membrane protein was observed in P. aeruginosa strains that showed uptake of liposomal rhodamine B, suggesting that this protein might have a role in the fusion of the liposomes with the outer membrane. [39] …”

Section: Fusogenic Liposomesmentioning

confidence: 99%

Lipid and polymer nanoparticles for drug delivery to bacterial biofilms

Forier

Raemdonck

Smedt

et al. 2014

Journal of Controlled Release

356

320

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“…[7][8][9][10] Upon incubation of a mixture of labeled liposomes and bacteria at 37°C, fluorescence intensity of Rh (590 nm) decreased and the NBD signal (520 nm) increased as time increased. 4 This indicates that a significant fusion occurred when fluid liposomes were mixed and incubated with bacteria at 37°C.…”

Section: Monitoring the Fusion Between Liposomes And Bacteriamentioning

confidence: 99%

“…[1][2][3][4][5][6] It was found that such enhanced bactericidal activity of fluid liposomal formulations is mainly due to their ability to fuse with bacterial cells and the fluid liposomes-bacterium fusion process has been confirmed by several different techniques. [7][8][9] It has recently been shown that several factors, such as bacterial membrane property, divalent cations, bacterial surface pH, and temperature can play important roles in fusion of fluid liposomes with bacteria. 10 There is a clear relationship between the degree of fluid in the liposomesbacterium fusion and the nature of the bacterial membrane, especially bacterial membrane lipid composition.…”

Section: Introductionmentioning

confidence: 99%

Enhanced bactericidal potency of nanoliposomes by modification of the fusion activity between liposomes and bacterium

Ma¹,

Wang²,

Zhao³

et al. 2013

IJN

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Background: Pseudomonas aeruginosa represents a good model of antibiotic resistance. These organisms have an outer membrane with a low level of permeability to drugs that is often combined with multidrug efflux pumps, enzymatic inactivation of the drug, or alteration of its molecular target. The acute and growing problem of antibiotic resistance of Pseudomonas to conventional antibiotics made it imperative to develop new liposome formulations to overcome these mechanisms, and investigate the fusion between liposome and bacterium. Methods: The rigidity, stability and charge properties of phospholipid vesicles were modified by varying the cholesterol, 1,2-dioleoyl-sn-glycero-3-phosphatidylethanolamine (DOPE), and negatively charged lipids 1,2-dimyristoyl-sn-glycero-3-phosphoglycerol sodium salt (DMPG), 1,2-dimyristoyl-sn-glycero-3-phopho-L-serine sodium salt (DMPS), 1,2-dimyristoyl-sn-glycero-3-phosphate monosodium salt (DMPA), nature phosphatidylserine sodium salt from brain and nature phosphatidylinositol sodium salt from soybean concentrations in liposomes. Liposomal fusion with intact bacteria was monitored using a lipid-mixing assay. Results: It was discovered that the fluid liposomes-bacterium fusion is not dependent on liposomal size and lamellarity. A similar degree of fusion was observed for liposomes with a particle size from 100 to 800 nm. The fluidity of liposomes is an essential pre-request for liposomes fusion with bacteria. Fusion was almost completely inhibited by incorporation of cholesterol into fluid liposomes. The increase in the amount of negative charges in fluid liposomes reduces fluid liposomes-bacteria fusion when tested without calcium cations due to electric repulsion, but addition of calcium cations brings the fusion level of fluid liposomes to similar or higher levels. Among the negative phospholipids examined, DMPA gave the highest degree of fusion, DMPS and DMPG had intermediate fusion levels, and PI resulted in the lowest degree of fusion. Furthermore, the fluid liposomal encapsulated tobramycin was prepared, and the bactericidal effect occurred more quickly when bacteria were cultured with liposomal encapsulated tobramycin. Conclusion:The bactericidal potency of fluid liposomes is dramatically enhanced with respect to fusion ability when the fusogenic lipid, DOPE, is included. Regardless of changes in liposome composition, fluid liposomes-bacterium fusion is universally enhanced by calcium ions. The information obtained in this study will increase our understanding of fluid liposomal action mechanisms, and help in optimizing the new generation of fluid liposomal formulations for the treatment of pulmonary bacterial infections.

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“…They can be also utilized in dermatology as a local drug depot in combination with penetration enhancers (de Leeuw et al, 2009;Pierre & Dos Santos Miranda Costa, 2011;Puglia & Bonina, 2012;Rahimpour & Hamishehkar, 2012). Furthermore, as antimicrobial drug delivery carriers, liposomes are able to enhance antibiotic concentrations at the site of infection (passive or active targeting), increase bactericidal efficacy (via fusion with the bacterial membrane), improve drug uptake and decrease the toxicity of potentially toxic antimicrobial agent (Fresta et al, 1995;Sachetelli et al, 2000;Mugabe et al, 2006b;Halwani et al, 2008, Drulis-Kawa et al, 2009.…”

Section: The Advantages Of Liposomes As Drug Carriersmentioning

confidence: 99%

Nanoliposome-based antibacterial drug delivery

Hallaj‐Nezhadi

Hassan

2013

Drug Delivery

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Although most bacterial infectious diseases can be treated successfully with the remarkable array of antibiotics, the microbial pathogens continue to be one of the most critical health challenges worldwide. One of the common efforts in addressing this issue lies in improving the existing antibacterial delivery systems since inefficient delivery can lead to poor therapeutic outcome of the administered drug. Recently, nanoliposomal systems have been widely used as promising strategies to overcome these challenges due to their unique set of properties. This article tries to briefly summarize the current studies that have taken advantage of liposomal nanoparticles as carriers to deliver antibacterial agents. The reviewed investigations demonstrate the immense potential of liposomal nanoparticles as carriers for antibiotic delivery and highlight the latent promise in this class of vehicles for treatment of bacterial infections. The future of these promising approaches lies in the development of more efficient techniques for preparing liposomal nanoparticles with great potential in effective and selective targeting of antibiotics to bacterial cells for eradication as well as the highest safety for human host.

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The interaction between Pseudomonas aeruginosa cells and cationic PC:Chol:DOTAP liposomal vesicles versus outer-membrane structure and envelope properties of bacterial cell

Cited by 57 publications

References 36 publications

Lipid and polymer nanoparticles for drug delivery to bacterial biofilms

Lipid and polymer nanoparticles for drug delivery to bacterial biofilms

Enhanced bactericidal potency of nanoliposomes by modification of the fusion activity between liposomes and bacterium

Nanoliposome-based antibacterial drug delivery

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