The Interaction Between Inhibitors of Nitric Oxide Synthase and Cyclooxygenase in Formalin-Induced Pain in Mice: An Isobolographic Study

Bhat, Abdul-Shakoor; Tandan, Surendra K.; Kumar, Dinesh; Krishna, V Rama; Prakash, Vellanki Ravi

doi:10.1213/ane.0b013e318163f71b

Cited by 10 publications

(4 citation statements)

References 29 publications

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“…Moreover, the dose of SMT (25 mg/kg, i.p.) that did not reduce the late phase (15-30 min) of formalin-induced pain behavior (Bhat et al 2008), induced a 50% reduction in the number of writhes in the model used herein.…”

Section: Discussionmentioning

confidence: 69%

“…In addition, it was shown that the selective inhibitor of inducible nitric oxide synthase produces an antinociceptive effect in the acetic-acid writhing test, suggestions that the nitric oxide produced by the inducible nitric oxide synthase plays a role in pain processing (LaBuda et al 2006). It was shown that SMT has a dose-dependent analgesic effect in the formalin-induced pain model as soon as 15-30 min after formalin injection (Bhat et al 2008). Moreover, the dose of SMT (25 mg/kg, i.p.)…”

Section: Discussionmentioning

confidence: 99%

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Inhibition of neuronal and inducible nitric oxide synthase does not affect the analgesic effects of NMDA antagonists in visceral inflammatory pain

Srebro¹,

Vučković²,

Prostran³

2016

Acta Neurobiologiae Experimentalis

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Previously we described the antinociceptive effect of magnesium sulfate and dizocilpine in the visceral and somatic rat models of pain. In the somatic model of pain, we established the influence of selective inhibitors of neuronal and inducible nitric oxide synthase on the antihyperalgesic effects of magnesium sulfate and dizocilpine. Therefore, the objective of the present study was to determine in the rat model of visceral pain whether same mechanisms are involved in the antinociceptive action of magnesium sulfate and dizocilpine.Analgesic activity was assessed using the acetic acid-induced writhing test in rats. Subcutaneous injection of either magnesium sulfate (15 mg/kg) or dizocilpine (0.01 mg/kg) decreased the number of writhes by about 60 and 70%, respectively. The role of nitric oxide on the effects of magnesium sulfate and dizocilpine was evaluated using selective inhibitor of neuronal [N-ω-Propyl-L-arginine hydrochloride (L-NPA)] and inducible [S-methylisothiourea (SMT)] nitric oxide synthase, which per se did not affect the number of writhes. We observed that the antinociceptive effect of magnesium sulfate or dizocilpine did not change in the presence of L-NPA (2 and 10 mg/kg, i.p.) and SMT (0.015 and 10 mg/kg, i.p.). We conclude that, nitric oxide produced by neuronal and inducible nitric oxide synthase does not modulate the effects of magnesium sulfate and dizocilpine in the visceral inflammatory model of pain in the rat.

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Section: Discussionmentioning

confidence: 69%

Section: Discussionmentioning

confidence: 99%

Inhibition of neuronal and inducible nitric oxide synthase does not affect the analgesic effects of NMDA antagonists in visceral inflammatory pain

Srebro¹,

Vučković²,

Prostran³

2016

Acta Neurobiologiae Experimentalis

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“…Antinociceptive activity has previously been shown for a NF-κB inhibitory compound, 2-chloroquinolinyl chalcone, TQ, [22] and for COX-2 and iNOS enzyme inhibitors in the formalin-induced paw licking test in mice [23,24]. This test characterizes the nociceptive response in two distinct phases involving neurogenic pain (I phase) and inflammatory pain (II phase) resulting from the release of inflammatory mediators in the peripheral tissues and functional changes in the neurons of the spinal dorsal horn [25].…”

Section: Discussionmentioning

confidence: 94%

The anti-inflammatory and antinociceptive effects of NF-κB inhibitory guanidine derivative ME10092

Dambrova

Zvejniece

Skapare

et al. 2010

International Immunopharmacology

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“…In recent times, there is much experimental evidence that the L-arginine-NO system plays an important role in the development of infl ammation and infl ammatory pain [10,11]. Nitric oxide (NO) is a short-lived signaling molecule that is formed from the amino acid L-arginine in the presence of NO-synthase (NOS).…”

Section: Introductionmentioning

confidence: 99%

L-Arginine-No System Participates in the Analgesic Effect of Flunixin Meglumine in the Rat

et al. 2016

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This study investigated whether the L-arginine-NO system participates in the analgesic effect of fl unixin meglumine in the rat. Hyperalgesia was induced by intraplantar (i.pl.) administration of carrageenan (500 μg) into the rat's hind paw. Electronic von Frey apparatus was used to determine paw withdrawal threshold induced by pressure as the painful stimulus, measured in grams (g). Flunixin meglumine (FM; 0.09-0.1 mg/kg; s.c.) and N G -nitro-L-arginine methyl ester (L-NAME; 10 mg/kg; i.p.), given separately as a pre-treatment, i.e. 15 min before i.pl. injection of carrageenan, produced a signifi cant antinociception. When FM (0.09 mg/kg) and a sub-effective dose of L-NAME (5 mg/kg) were co-administered, the antinociceptive effect was signifi cantly increased in comparison with the effect of FM alone. L-arginine (L-ARG;10 mg/kg; i.p.) itself did not produce signifi cant effect on carrageenan-induced hyperalgesia, but signifi cantly reduced the antinociceptive effects of both FM and FM + L-NAME combination. The inhibition of the production of NO might be involved in the mechanism of the analgesic effect of FM.

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The Interaction Between Inhibitors of Nitric Oxide Synthase and Cyclooxygenase in Formalin-Induced Pain in Mice: An Isobolographic Study

Abstract: Our results explicitly indicate the synergistic nature of the interaction between NOS and COX inhibitors in formalin-induced nociceptive behavior in mice, and provide an alternative approach for controlling pain.

Cited by 10 publications

References 29 publications

Inhibition of neuronal and inducible nitric oxide synthase does not affect the analgesic effects of NMDA antagonists in visceral inflammatory pain

Inhibition of neuronal and inducible nitric oxide synthase does not affect the analgesic effects of NMDA antagonists in visceral inflammatory pain

The anti-inflammatory and antinociceptive effects of NF-κB inhibitory guanidine derivative ME10092

L-Arginine-No System Participates in the Analgesic Effect of Flunixin Meglumine in the Rat

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