The integration characteristics of the exogenous growth hormone gene in a transgenic common carp (Cyprinus carpio L.) with fast-growth performance

Chen, Ji; Luo, Qing; Bao, Hairong; Liao, Lanjie; Li, Yongming; Zhang, Zhu; Wang, Yaping; Hu, Wei

doi:10.1007/s11434-015-0893-x

Cited by 9 publications

(3 citation statements)

References 46 publications

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“…The transgene transmission was more than expected for Mendelian segregation of single locus. This pattern suggested that the transgene DNA might be integrated at multiple loci in founder fish, and thus inherited stably (Chen et al, 2015;Devlin et al, 2004). The results showed that the body length and weight growth of all transgenic crosses (♂NT × ♀TG; ♂TG × ♀NT; ♂TG × ♀TG) were significantly higher compared to the non-transgenic control (♂NT ×♀ NT) (Table 4).…”

Section: Transgene Transmissionmentioning

confidence: 93%

Gene Transmission, Growth, and Exogeneous Growth Hormone Expression of G2 Transgenic Betta Fish (Betta imbellis)

Ayuningthias

Nasrullah

Soelistyowati

et al. 2021

JIPK

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Highlight ResearchThe F2 of GH-transgenic B. imbellis was successfully producedThe transgene inheritance by the F2 fish was more than 90%The growth and body size of transgenic fish was significantly higher than controlF2 fish reached a larger body size in a shorter period compared to the F1 AbstractIn our previous research, we had successfully produced G0 and G1 Pangasianodon hypophthalmus growth hormone (PhGH) transgenic B. imbellis, native ornamental betta from Indonesia, which its giant-sized variant has valuable price for the breeders. The G0 and G1 transgenic (TG) fish showed higher growth rate and body size compared to the non-transgenic (NT) fish. The study was aimed to produce and evaluate the consistencies of transgene transmission and expression in G2 generation. The growth rate and body size between TG and NT fish was also compared. The G2 generation was produced using crosses between TG and NT G1 fish: ♂TG × ♀TG, ♂TG × ♀NT, ♂NT × ♀TG, and ♂NT ×♀ NT. Fish were reared for 12 weeks, and transgene detection was performed using the polymerase chain reaction method (PCR) on isolated DNA from the caudal fin clips. The endogenous and exogenous GH expression analysis was conducted using the quantitative real-time PCR (qPCR) method. The results showed that the inheritance of the GH transgene by the G2 fish was more than 90% in all transgenic crosses. Endogenous GH was expressed at the same levels in the brain of TG and NT fish, but the exogenous GH expression was highly detected only in the TG fish. The G2 transgenic fish had a higher specific growth rate, up to 31%, compared to the control. The body length of TG crosses were 23−35% higher and had 111−135% higher body weight compared to NT fish. These results showed a promising approached in mass-producing stable lines of giant-sized betta using the GH-transgenic technology.

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Section: Transgene Transmissionmentioning

confidence: 93%

Gene Transmission, Growth, and Exogeneous Growth Hormone Expression of G2 Transgenic Betta Fish (Betta imbellis)

Ayuningthias

Nasrullah

Soelistyowati

et al. 2021

JIPK

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“…The transmission of exogenous transgene from one generation to the next depends on stable integration into host genomes. Expression of the exogenous gene may produce a phenotypic improvement of transgenic fish (Chen et al 2015). A high percentage of transgene transmission is vital for ensuring inheritance of exogenous genes in future generations.…”

Section: Stability Of Cggh Expression and Inheritance In G 3 Transgenmentioning

confidence: 99%

Growth hormone transgenesis and feed composition influence growth and protein and amino acid content in transgenic G3 mutiara catfish (Clarias gariepinus)

2021

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Clarias gariepinus growth hormone (CgGH) transgenesis was previously used to develop a population of second-generation (G2) transgenic mutiara catfish (C. gariepinus). The third generation of these fish (1-month old fingerlings) had 2–3 times improvement in growth compared to non-transgenic fish in the commercial feed test for 6 weeks of rearing. We assessed the impact of CgGH transgene expression on growth and protein and amino acid content of the G3 generation of these transgenic mutiara catfish relative to non-transgenic catfish. Since variation in composition of feed mixes can affect protein and amino acid content of fish, we tested three mixtures of commercial feed and boiled tuna (Euthynnus affinis): feed A (50:50 feed to tuna), B (65:35), and C (80:20) to transgenic catfish. Feed A* (50:50) was fed as a control to non-transgenic catfish. Feed efficiency, including feed conversion ratio and protein use efficiency (i.e., protein retention and protein productive value), was assessed. Feed efficiency, protein content, and essential amino acid content in G3 transgenic catfish (feed A and B) were higher than in non-transgenic fish (feed A*). The latter were deficient in lysine and methionine. Transgenic catfish fed with feed C (80:20) showed lysine deficiency and lower growth than fish fed feeds A and B. Feed B (65:35) was the optimal feed mixture utilized; it increased growth, protein levels, and feed conversion efficiency in G3 catfish. The growth of transgenic fish was higher than non-transgenic fish when supported by feeding with balanced nutrients.

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“…Study of fast-growing transgenic carp showed that although their offspring carried the same integration site (Chen et al 2015), their growth traits significantly varied and the frequency distribution of their body weight and length presented two obvious peaks (unpublished data). We speculate that growth traits are mainly associated with the genotypes of specific genes in the genome of the receptor (fertilized eggs undergoing microinjection).…”

Section: Introductionmentioning

confidence: 99%

Selection of growth-related genes and dominant genotypes in transgenic Yellow River carp Cyprinus carpio L.

Luo

Huang

Zhang

et al. 2018

Funct Integr Genomics

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Transgenic Yellow River carp is characterized by rapid growth rate and high feed-conversion efficiency and exhibits a great application prospect. However, there is still a significant separation of growth traits in the transgenic Yellow River carp family; as such, growth-related genotypes must be screened for molecular marker-assisted selection. In this study, 23 growth-related candidate genes containing 48 SNP markers were screened through bulked segregant analysis (BSA) among transgenic Yellow River carp family members showing significant separation of growth traits. Then, two growth-related genes (Nos. 17 and 14 genes) were identified through combined genome-wide association study (GWAS) of candidate genes and validation of the full-sibling family approach. Nos. 17 and 14 genes encode BR serine/threonine-protein kinase 2 (BRSK2) and eukaryotic translation-initiation factor 2-alpha kinase 3 (Eif2ak3), respectively. The average body weight of three subgroups carrying the genotypes 17GG, 17GG + 14CC, and 17GG + 14TT of these two genes increased by 27.96, 38.28, and 33.72%, respectively, compared with the controls. The proportion of individuals with body weight > 500 g in these subgroups increased by 19.22, 26.82, and 30.92%, respectively. The results showed that appropriate genotype carriers can be selected from the progeny population through BSA sequencing combined with simplified GWAS analysis. Hence, basic population for breeding can be constructed and transgenic Yellow River carp strains with stable production performance and uniform phenotypic properties can be bred.Electronic supplementary materialThe online version of this article (10.1007/s10142-018-0597-9) contains supplementary material, which is available to authorized users.

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The integration characteristics of the exogenous growth hormone gene in a transgenic common carp (Cyprinus carpio L.) with fast-growth performance

Cited by 9 publications

References 46 publications

Gene Transmission, Growth, and Exogeneous Growth Hormone Expression of G2 Transgenic Betta Fish (Betta imbellis)

Gene Transmission, Growth, and Exogeneous Growth Hormone Expression of G2 Transgenic Betta Fish (Betta imbellis)

Growth hormone transgenesis and feed composition influence growth and protein and amino acid content in transgenic G3 mutiara catfish (Clarias gariepinus)

Selection of growth-related genes and dominant genotypes in transgenic Yellow River carp Cyprinus carpio L.

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