The integrated and free states of Streptomyces griseus plasmid pSG1

Biological and Molecular Investigations of Spontaneously Developing Pocks in Streptomyces.

“…We reported that BC had a remarkable sporulation-stimulating effect with striking elongation of the aerial mycelia in S. azureus 22) : BC also had a stimulating effect on submerged mycelial growth 28) . The number of spores of pSA1.1-carrying strain PK100 was increased 100-fold or more (5x10 4 …”

“…This phenomenon had been designated as "unknown physical phenomenon", but was known as "lethal zygosis" or "pocks" in the cases of Streptomyces coelicolor, Streptomyces lividans and some others 2,3,4,9) , in which conjugative plasmids and their transfer are involved in lethal zygosis. The pocks are characterized by circular zones of retarded development of the plasmid-accepted hyphae (transconjugants), when the donor strain develops within the lawn of the recipient strain (plasmidless or plasmid-cured strain) 2,3,4,9,12,16,29) .…”

“…The pocks are characterized by circular zones of retarded development of the plasmid-accepted hyphae (transconjugants), when the donor strain develops within the lawn of the recipient strain (plasmidless or plasmid-cured strain) 2,3,4,9,12,16,29) . Much interest has focused on suppression of aerial mycelial growth and sporulation by conjugative plasmids in several Streptomyces species 8,9,15,16,17) .…”

Characterization of the attP site of the integrative element pSAM2 from Streptomyces ambofaciens

“…Such elements have been isolated from various actinobacteria belonging to the genera Streptomyces, Saccharopolyspora, Micromonospora and Amycolatopsis. The integration and excision processes have been studied in some of these elements [pSAM2 (Boccard et al, 1988), SLP1 (Omer & Cohen, 1986), pMEA100 (Madon et al, 1987), pSG1 (Cohen et al, 1985), pIJ408 (Sosio et al, 1989), pSE211 (Brown et al, 1990), pSE101 (Brown et al, 1994) and pMEA300 (Vrijbloed et al, 1994)] and shown to involve site-specific recombination.…”

Effects of UV Dose on Formation of Spontaneously Developing Pocks inStreptomyces azureusATCC14921

“…The phenomenon seems to be related to another kind of pocks (called C pocks here) [7][8][9][10] in which conjugative plasmids and their transfer are involved. Both kinds of pocks have circular zones of retarded development of plasmidaccepted hypha.…”