The insertion of the Agrobacterium rhizogenes rolC gene in tomato (Solanum lycopersicum L.) affects plant architecture and endogenous auxin and abscisic acid levels

Bettini, P.; Baraldi, Rita; Rapparini, Francesca; Melani, Lorenzo; Mauro, Maria Luisa; Bindi, Daniela; Buiatti, M.

doi:10.1016/j.scienta.2009.09.013

Cited by 29 publications

(19 citation statements)

References 41 publications

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“…For example, Bettini et al (2010) reported that a lower IAA/ ABA ratio in the rolC-transgenic tomato can lead to reduced vegetative growth. A relationship between shoot development and the ratio of these two hormones was observed in lemon scions, in which a vigorous rootstock had a higher IAA/ABA ratio in the new shoots (Noda et al, 2000).…”

Section: Discussionmentioning

confidence: 99%

Elevated Carbon Dioxide and/or Ozone Concentrations Induce Hormonal Changes in Pinus tabulaeformis

Zhang

et al. 2011

J Chem Ecol

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We investigated endogenous plant hormones and needle growth in Pinus tabulaeformis plants grown in open-top chambers and exposed to ambient or elevated concentrations of carbon dioxide (CO(2)) and/or ozone (O(3)). Exposure to elevated CO(2) for 100 days significantly increased the change in fresh needle weight, indole-3-acetic acid (IAA), isopentenyl-adenosine (iPA), and dihydrozeatin riboside (DHZR) content. Abscisic acid (ABA) content decreased, and no effect was observed on zeatin riboside (ZR) content or changes in needle dry weight. The ratios of IAA/ABA and total cytokinins (CKs)/ABA [Formula: see text] were increased. Elevated O(3) significantly decreased IAA and ZR, and decreased the ratios of IAA/ABA and CKs/ABA. Ozone treatment increased ABA content but did not change iPA or DHZR content or change fresh or dry needle weights. The combination treatment significantly increased ABA content and the IAA/ABA ratio but decreased the total CKs/ABA ratio and had no effect on CKs or IAA content or change in fresh and dry needle weights. The results indicate that elevated CO(2) ameliorated the effects of elevated O(3) on tree growth.

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Section: Discussionmentioning

confidence: 99%

Elevated Carbon Dioxide and/or Ozone Concentrations Induce Hormonal Changes in Pinus tabulaeformis

Zhang

et al. 2011

J Chem Ecol

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“…For instance, a constitutively modified endogenous hormonal background has been shown to affect the active defence response to fungal pathogens in tomato plants transgenic for the Agrobacterium rhizogenes rol genes (Bettini et al, 2003(Bettini et al, , 2010 and in rol-transformed cells of various Solanaceae, Araliaceae, Rubiaceae, Vitaceae and Rosaceae. In fact, the modified hormonal profile upregulates the activation of secondary metabolites involved in stress responses (Bulgakov, 2008).…”

Section: Introductionmentioning

confidence: 99%

Response to metal stress of Nicotiana langsdorffii plants wild-type and transgenic for the rat glucocorticoid receptor gene

Fuoco

Bogani

Capodaglio

et al. 2013

Journal of Plant Physiology

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a b s t r a c tRecently our findings have shown that the integration of the gene coding for the rat gluco-corticoid receptor (GR receptor) in Nicotiana langsdorffii plants induced morphophysiological effects in transgenic plants through the modification of their hormonal pattern. Phytohormones play a key role in plant responses to many different biotic and abiotic stresses since a modified hormonal profile up-regulates the activation of secondary metabolites involved in the response to stress. In this work transgenic GR plants and isogenic wild type genotypes were exposed to metal stress by treating them with 30 ppm cadmium(II) or 50 ppm chromium(VI). Hormonal patterns along with changes in key response related metabolites were then monitored and compared. Heavy metal up-take was found to be lower in the GR plants. The transgenic plants exhibited higher values of S-abscisic acid (S-ABA) and 3-indole acetic acid (IAA), salicylic acid and total polyphenols, chlorogenic acid and antiradical activity, compared to the untransformed wild type plants. Both Cd and Cr treatments led to an increase in hormone concentrations and secondary metabolites only in wild type plants. Analysis of the results suggests that the stress responses due to changes in the plant's hormonal system may derive from the interaction between the GR receptor and phytosteroids, which are known to play a key role in plant physiology and development.

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“…These effects include aberrant phenotype (Koperdáková et al 2009), superfluous leaf and flower morphology (Carmi et al 2003), floral infertility and pollen viability (Casanova et al 2005;Zhang et al 2006), among others. In addition, desirable characteristics such as early flowering (Godo et al 1997), short internode (Mishiba et al 2006;van Altvorst et al 1992), apomictic embryogenesis (Casanova et al 2004), parthenocarpy (Carmi et al 2003), short plant life span, and high yield (Bettini et al 2010;Bulgakov et al 2005) were also observed. The variations in observed phenotype of rol genes carrying plants has been attributed to variations in the site of gene insertion, copy number of the T-DNA, and the genome of transformed cells (Tanaka et al 2001).…”

Section: Introductionmentioning

confidence: 99%

Expression of rol genes in transgenic soybean (Glycine max L.) leads to changes in plant phenotype, leaf morphology, and flowering time

Zia

Mirza

Malik

et al. 2010

Plant Cell Tiss Organ Cult

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Soybean (Glycine max L.) cultivar NARC-4 was transformed with constructs carrying rolA, rolB, or rolC genes, each under the control of the Cauliflower Mosaic Virus 70S promoter. Cotyledonary nodes of soybean seeds were infected with Agrobacterium tumefaciens strain LBA4404 carrying one of the three rol genes, along with nptII in the plasmid pLBR. The efficiency of the transformation varied slightly among the three constructs, with frequencies of 6, 7, and 5% for the rolA, rolB, and rolC genes, respectively, being observed. Southern blot analysis confirmed the integration of rol genes in the soybean genome with varying numbers of copies of the transgene. All transformed plants showed enhanced rooting, but the number of adventitious roots was higher for transformants carrying the rolB transgene. rolA and rolC transformants showed dwarf phenotypes, clustered branching, and variations in leaf morphology. Furthermore, these plants flowered within a short period of time and produced lower numbers of flowers. rolB transformants showed variations in phenotype, including dwarf to semidwarf and shrubby growth, abnormal stem growth, short internodes, variations in leaf morphology, and greenish to yellowish-green colored leaves. These plants also flowered early, but dwarf plants produced low numbers of flowers, while shrubby plants produced high numbers of flowers, but these were mostly infertile.

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The insertion of the Agrobacterium rhizogenes rolC gene in tomato (Solanum lycopersicum L.) affects plant architecture and endogenous auxin and abscisic acid levels

Cited by 29 publications

References 41 publications

Elevated Carbon Dioxide and/or Ozone Concentrations Induce Hormonal Changes in Pinus tabulaeformis

Elevated Carbon Dioxide and/or Ozone Concentrations Induce Hormonal Changes in Pinus tabulaeformis

Response to metal stress of Nicotiana langsdorffii plants wild-type and transgenic for the rat glucocorticoid receptor gene

Expression of rol genes in transgenic soybean (Glycine max L.) leads to changes in plant phenotype, leaf morphology, and flowering time

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