The Ins and Outs of Autophagic Ribosome Turnover

Kazibwe, Zakayo; Liu, Ang-Yu; MacIntosh, Gustavo C.; Bassham, Diane C.

doi:10.3390/cells8121603

Cited by 20 publications

(19 citation statements)

References 97 publications

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“…Several cellular pathways described in eukaryotes illustrate the rRNA degradation for the control of a proper translation, cell homeostasis or in response to environmental stresses. Ribosomes are the target that leads to rRNA degradation by selective or non-selective pathways such as RNautophagy, macroautophagy, or ribophagy (Kazibwe et al, 2019). It has been demonstrated that the phenomenon is not related to autophagy/ribophagy mechanism (Abrahão et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

How Tupanvirus Degrades the Ribosomal RNA of Its Amoebal Host? The Ribonuclease T2 Track

Rolland¹,

Scola²,

Levasseur³

2020

Front. Microbiol.

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Tupanviruses are giant viruses recently discovered in Brazil from extreme environments: Tupanvirus soda lake (TPV-SL) and Tupanvirus deep ocean (TPV-DO). Unexpected features in Tupanviruses is the cytotoxic effect observed during infection, where the virus degrades the ribosomal RNA (rRNA) of its amoebal host. Interestingly, only TPV-SL causes this rRNA shutdown. We performed a genomic comparison of the two strains to determine potential modifications explaining the absence of rRNA degradation by TPV-DO. Whole genome comparisons were performed as well as more in-depth analysis at the gene level. We also calculated selective pressure on the orthologous genes between the two viruses. Our computational and evolutionary investigations revealed a potential target: a ribonuclease T2. These enzymes are known to be involved in cellular RNA catabolism such as in lysosomal degradation of rRNA. Our results suggest a functional ribonuclease localized in acid compartment closely related to ribonuclease T2 from eukaryotes. Silencing of the RNAse T2 gene of TPV-SL abolished its rRNA shutdown ability thereby correlating in silico assumption to the experimental evidence. In conclusion, all our results pointed to RNAse T2 as a target for explaining the difference for rRNA degradation ability between both strains.

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Section: Introductionmentioning

confidence: 99%

How Tupanvirus Degrades the Ribosomal RNA of Its Amoebal Host? The Ribonuclease T2 Track

Rolland¹,

Scola²,

Levasseur³

2020

Front. Microbiol.

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“…It has been previously reported that rns 2-2 plants, which lack the T2 ribonuclease RNS2, display enhanced basal autophagy. The mutants were larger than WT plants, and the authors postulated this was due to higher expansin levels [ 72 ]. The current results suggest that autophagy is involved in expansin maintenance.…”

Section: Resultsmentioning

confidence: 99%

Exploring the Contribution of Autophagy to the Excess-Sucrose Response in Arabidopsis thaliana

Laloum

Magen

Soroka

et al. 2022

IJMS

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Autophagy is an essential intracellular eukaryotic recycling mechanism, functioning in, among others, carbon starvation. Surprisingly, although autophagy-deficient plants (atg mutants) are hypersensitive to carbon starvation, metabolic analysis revealed that they accumulate sugars under such conditions. In plants, sugars serve as both an energy source and as signaling molecules, affecting many developmental processes, including root and shoot formation. We thus set out to understand the interplay between autophagy and sucrose excess, comparing wild-type and atg mutant seedlings. The presented work showed that autophagy contributes to primary root elongation arrest under conditions of exogenous sucrose and glucose excess but not during fructose or mannitol treatment. Minor or no alterations in starch and primary metabolites were observed between atg mutants and wild-type plants, indicating that the sucrose response relates to its signaling and not its metabolic role. Extensive proteomic analysis of roots performed to further understand the mechanism found an accumulation of proteins essential for ROS reduction and auxin maintenance, which are necessary for root elongation, in atg plants under sucrose excess. The analysis also suggested mitochondrial and peroxisomal involvement in the autophagy-mediated sucrose response. This research increases our knowledge of the complex interplay between autophagy and sugar signaling in plants.

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“…Moreover, pathogens are targeted for degradation by selective autophagy. Cargo-specific Life 2021, 11, 839 2 of 19 names have been used to classify the various types of selective autophagy (e.g., pexophagy, mitophagy, aggrephagy, glycophagy, lipophagy ribophagy and xenophagy) [9,10]. In particular, mitophagy is strongly associated with tumorigenesis [11,12] (Figure 1).…”

Section: Introductionmentioning

confidence: 99%

Autophagy Modulators in Cancer: Focus on Cancer Treatment

Nam

2021

Life

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Uncontrolled autophagy has been associated with the development and progression of various cancers that are resistant to cancer therapy. Therefore, many efforts to modulate uncontrolled autophagy as a cancer treatment have been attempted, from basic science to clinical trials. However, it remains difficult to equally apply autophagy modulators to cancer therapy because autophagy is a double-edged sword in cancer: it can be tumor-suppressive or tumor-protective. Therefore, the precise mechanisms of autophagy modulators and their varied responsiveness to each cancer type should be addressed in detail. This study will describe the precise mechanisms of developing various autophagy modulators, their current therapeutic applications and future perspectives.

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The Ins and Outs of Autophagic Ribosome Turnover

Cited by 20 publications

References 97 publications

How Tupanvirus Degrades the Ribosomal RNA of Its Amoebal Host? The Ribonuclease T2 Track

How Tupanvirus Degrades the Ribosomal RNA of Its Amoebal Host? The Ribonuclease T2 Track

Exploring the Contribution of Autophagy to the Excess-Sucrose Response in Arabidopsis thaliana

Autophagy Modulators in Cancer: Focus on Cancer Treatment

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