The inner life of integrin adhesion sites: From single molecules to functional macromolecular complexes

Recent Advances and Prospects in the Research of Nascent Adhesions

“…Resolution of these open questions requires new techniques that can deal with the fast molecular turnover within NAs. However, this is still a significant challenge for the single molecule localization microscopy (Orré et al, 2019) such as PALM (Betzig et al, 2006), stochastic optical reconstruction microscopy (Rust et al, 2006) or super-resolution optical fluctuation imaging (Dertinger et al, 2009). First promising insights into the dynamics of NAs nevertheless were provided by the single particle tracking PALM (Inavalli et al, 2019), which revealed integrin cycling between free diffusion and immobilization, while transient interaction with talins promoted integrin activation and immobilization (Rossier et al, 2012).…”

Adhesion force spectroscopy with nanostructured colloidal probes reveals nanotopography-dependent early mechanotransductive interactions at the cell membrane level

“…In recent years, it became evident that mechanical stimuli of the ECM, such as rigidity and/or spatial organisation and dimensionality of adhesion sites (e.g., in terms of geometry and topography), alter intrinsic cellular properties, such as the actin cytoskeletal organisation/mechanics and the signalling status [7][8][9][10] .The intricate processes through which the cell perceives and reacts to mechanical and structural stimuli in its microenvironment were termed mechanosensing and mechanotransduction [11][12][13][14][15][16][17][18] .…”

Cells nanomechanics by atomic force microscopy: focus on interactions at nanoscale

“…For instance, 'molecular clutch' model is considering the coupling of adaptor protein talin with actomyosin motor for transmission of force. For higher tension, talin can unfold binding vinculin, in turn recruiting components and reinforcing IAC formation [83][84][85]. Indeed, AFM nanomechanics can be used successfully to quantify the reconfiguration of cytoskeleton induced by mechanotransduction events.…”