The innate immune response to grass carp hemorrhagic virus (GCHV) in cultured Carassius auratus blastulae (CAB) cells

Fish virus-induced interferon exerts antiviral function through Stat1 pathway

“…Grass carp hemorrhagic virus (GCHV), a dsRNA virus in the family Reoviridae, was propagated in CAB cells. Virus titers were detected in CAB cells and UV inactivation of GCHV was executed as described previously (Zhang et al, 2007).…”

“…Protein concentration was determined using the BCA TM protein assay kit (PIERCE) with bovine serum albumin (BSA) as a standard. The titration of recombinant IFN was measured by monitoring the inhibition of the cytopathic effect (CPE) caused by GCHV according to the previous report (Zhang et al, 2007). The purified recombinant protein was used for all IFN antiviral assays.…”

“…Recently virusinduced IFN genes have been characterized in various fish species (Aggad et al, 2009;Altmann et al, 2003;Long et al, 2004;Lutfalla et al, 2003;Robertsen et al, 2003;Zou et al, 2007), and some downstream ISG counterparts have also been identified (Robertsen, 2006;Zhang et al, 2007), strongly indicating that teleost fish possess an IFN antiviral system. In support of this notion, IFN inducible genes including Mx and PKR have been proved to block virus replication in fish (Caipang et al, 2003;Larsen et al, 2004;Zhu et al, 2008), and there exists a conserved RLR (RIG-I-like receptors) pathway responsible for fish IFN induction during virus infection (Biacchesi et al, 2009).…”

“…This study aimed to investigate the function of a crucian carp (Carassius auratus L.) IFN by asking how it exerts its antiviral effect. We have previously identified by suppressive subtractive hybridization a set of genes, which are the counterparts of key genes involved in mammalian IFN antiviral response (Zhang et al, 2007;Zhang et al, 2003b). In the current study, we show that crucian carp IFN belongs to group I of fish virus-induced IFNs, and similar to mammalian IFNs.…”

Molecular cloning, characterization and expression analysis of the PKZ gene in rare minnow Gobiocypris rarus

“…Crucian carp PKZ is detected at a very low level of constitutive expression and can be induced by GCRV, poly(I:C) or interferon in CAB cells [28,29]. Zebrafish PKZ is transcribed constitutively at low levels and is highly induced after injection of poly(I:C).…”