The initiation of callus and regeneration from callus culture ofTulipa gesneriana

Abstract

Cold treatment of seeds, obtained from crosses between cultivars of T. gesneriana L., affects the developmental stage of embryos, which in turn influences the frequency of callus induction and the development of different callus types. Cold-treated, mature embryos and basal segments of in vitro-derived bulblets, were suitable explants for the initiation of regenerative callus on medium with 2,4-dichlorophenoxyacetic acid. The bulblets were initiated on flower-stalk segments from cold-stored bulbs of T. gesneriana 'Christmas Marvel.' Histological analyses of regenerative callus revealed the regeneration of bulb-like structures. The influences of culture medium, culture conditions, growth regulators and acetylsalicylic acid, an inhibitor of ethylene, on the initiation and establishment of regenerative callus cultures are discussed.

Somatic embryogenesis in tulip (Tulipa gesneriana L.) flower stem cultures

“…However, at the lowest concentration of 2,4-D (1 μM), adventitious roots developed. Famelaer et al (1996) obtained similar results in in vitro tulip culture. Root formation was observed on Tulipa "Christmas Marvel" calluses cultured on medium with 2,4-D, but not on medium with Picloram.…”

“…al. 1992;Kuijpers and Langens-Gerrits, 1997;Le Nard and Chanteloube, 1992;Podwyszyńska, 2003) or immature embryos isolated from mature seeds (Famelaer, 1996) has been reported, but the propagation factor and the growth rate were still low. The most convenient way of regeneration is through somatic embryogenesis.…”

Induction of bulb organogenesis in in vitro cultures of tarda tulip (Tulipa tarda Stapf.) from seed-derived explants

“…According to De Hertogh and Le Nard ( 1993 ), germination begins within a few days of seed hydration, as also confirmed with T. kaufmanniana (Rouhi et al 2010 ). Cold-treated seeds of Tulipa gesneriana germinated after 5–6 wk (Famelaer et al 1996 ). In this study, germination was not observed until 6 mo, as in nature.…”

“…The morphogenic response of the explants depends on the presence and concentration of growth regulators in the medium (Gaspar et al 2003 ). To initiate organogenesis in tulips, BAP is usually used, either alone or with auxin (Wright and Alderson 1980 ; Taeb and Alderson 1990 ; Minas 2007 ), although Famelaer et al ( 1996 ) used only picloram and 2,4-dichlorophenoxyacetic acid (2,4-D) to obtain callus tissue. In this study, only 0.5 μM BAP was used to stimulate organogenesis.…”

Clonal propagation of Lomandra longifolia by somatic embryogenesis

“…(Krikorian and Kann, 1981), Lilium spp. (Priyadarshi and Sen, 1992;Wickremesinhe et al, 1994;Godo et al, 1998;Nakano et al, 2000) and Tulipa gesneriana (Famelaer et al, 1996). Somatic embryogenesis protocols have also been developed for several species of native Australian rushes (Panaia et al, 2004a,b).…”