The initiation of bud burst in grapevine features dynamic regulation of the apoplastic pore size

Signorelli, Santiago; Shaw, Jeremy; Hermawaty, Dina; Wang, Zi; Verboven, Pieter; Considine, John A.; Considine, Michael

doi:10.1093/jxb/erz200

Cited by 24 publications

(18 citation statements)

References 39 publications

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“…Our earlier studies have indicated that oxygen and ethylene signalling ( Meitha et al , 2015 , 2018 ) and cell–cell transport ( Signorelli et al , 2020 ) may have key regulatory roles in bud development. In addition, we have reviewed the role of cell cycle regulation as a master or slave of dormancy transitions ( Velappan et al , 2017 ).…”

Section: Resultsmentioning

confidence: 99%

The bud dormancy disconnect: latent buds of grapevine are dormant during summer despite a high metabolic rate

Velappan

Chabikwa

Considine

et al. 2022

Journal of Experimental Botany

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Grapevine (Vitis vinifera L.) displays wide plasticity to climate, however the physiology of dormancy along a seasonal continuum is poorly understood. Here we investigated the apparent disconnect between dormancy and the underlying respiratory physiology and transcriptome of grapevine buds, from bud set in summer to bud burst in spring. The establishment of dormancy in summer was pronounced and reproducible, however this was coupled with little or no change in physiology, indicated by respiration, hydration and tissue oxygen tension. The release of dormancy was biphasic; the depth of dormancy declined substantially by mid-autumn, while the subsequent decline towards spring was moderate. Observed changes in physiology failed to explain the first phase of dormancy decline, in particular. Transcriptome data contrasting development from summer through to spring also indicated that dormancy was poorly reflected by metabolic quiescence during summer and autumn. Gene ontology and enrichment data revealed the prevailing influence of abscisic acid (ABA)-related gene expression during the transition from summer to autumn, and promoter motif analysis suggested photoperiod may play an important role in regulating ABA functions during the establishment of dormancy. Transcriptomic data from later transitions reinforced the importance of oxidation and hypoxia as physiological cues to regulate the maintenance of quiescence and resumption of growth. Collectively these data reveal a novel disconnect between growth and metabolic quiescence in grapevine following bud set, which requires further experimentation to explain the phenology and dormancy relationships.

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Section: Resultsmentioning

confidence: 99%

The bud dormancy disconnect: latent buds of grapevine are dormant during summer despite a high metabolic rate

Velappan

Chabikwa

Considine

et al. 2022

Journal of Experimental Botany

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“…This was consistent with the hypothesis that water availability was an important variable affecting bud burst kinetics in single-node cutting experiments. It is well documented that quiescent buds are isolated to prevent dehydration during winter, particularly through the restriction of aquaporin activity, plasmodesmata conductance, and apoplastic conductivity [6][7][8][9][10]. For water to have a direct effect on bud burst, it needs to be transported to the bud after transferring the single-node cutting to the seedling tray.…”

Section: Resultsmentioning

confidence: 99%

“…In terms of GWC, 85% of FC in our system represented 0.94 g water •g soil −1 and 100% of FC was 1.11 g water •g soil −1 . Several earlier studies have illustrated the regulated changes in free and bound water [10], in the activity of aquaporin transporters [6,11], the role of plasmodesmata in dormancy transitions of perennating buds [7], the expansion of apoplastic connectivity upon bud burst [9], and the change dynamics in moisture content in the bud across the season [11]. The fact that water has a strong effect on bud burst in explant further supports the importance of those biological processes controlling water content in the bud, such as plasmodesmata regulation, aquaporins activity, etc.…”

Section: Discussionmentioning

confidence: 99%

Soil Water Content Directly Affects Bud Burst Rate in Single-Node Cuttings of Perennial Plants

2022

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The use of single-node cuttings of shoots as explants to study bud dormancy and its physiology under controlled conditions is a common practice in grapevine (Vitis vinifera L.) or other perennial plant research. In particular, this method has been extensively used to understand the effect of different chemicals on bud dormancy and bud burst. However, the soil water content in those experiments is usually not reported and its relevance is often neglected. Here, we observed that an unevenly distributed soil water content in a tray containing multiple explants results in an uneven pattern of bud burst within the same treatment. Thus, we hypothesised that soil water content can dramatically affect bud burst. To investigate this, we first established that fresh single-node cuttings were able to transport water into the buds. We then tested the rate of bud burst at different water treatments (35%, 55%, 70%, 85%, and 100% of field capacity; FC). We observed a clear dependence of bud burst on water, in which, at very low levels of water, bud burst does not occur; after 35% FC, bud burst rate increases with water content until around 85% FC; and, from 85% FC, bud burst rate becomes independent of water content. These data highlight the critical importance of monitoring soil water content in any bud burst assay in perennials. Finally, we provide a detailed protocol for determining and controlling field capacity and other soil water content indicators.

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“…Globally, grapes are considered one of the most economically important and vital fruit species and grown in almost 100 countries. Grapes are consumed in different forms, as fresh, dried (raisins), and processed food and beverages (juices and soft drinks) ( Signorelli et al, 2019 ). The current climatic anomalies and scenarios are a threat and can lead to unpredicted and urgent provocations to the grapevine’s traditional cultivation ( Leolini et al, 2018 ).…”

Section: Introductionmentioning

confidence: 99%

Silicon Alleviate Hypoxia Stress by Improving Enzymatic and Non-enzymatic Antioxidants and Regulating Nutrient Uptake in Muscadine Grape (Muscadinia rotundifolia Michx.)

et al. 2021

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Flooding induces low oxygen (hypoxia) stress to plants, and this scenario is mounting due to hurricanes followed by heavy rains, especially in subtropical regions. Hypoxia stress results in the reduction of green pigments, gas exchange (stomatal conductance and internal CO2 concentration), and photosynthetic activity in the plant leaves. In addition, hypoxia stress causes oxidative damage by accelerating lipid peroxidation due to the hyperproduction of reactive oxygen species (ROS) in leaf and root tissues. Furthermore, osmolyte accumulation and antioxidant activity increase, whereas micronutrient uptake decreases under hypoxia stress. Plant physiology and development get severely compromised by hypoxia stress. This investigation was, therefore, aimed at appraising the effects of regular silicon (Si) and Si nanoparticles (SiNPs) to mitigate hypoxia stress in muscadine (Muscadinia rotundifolia Michx.) plants. Our results demonstrated that hypoxia stress reduced muscadine plants’ growth by limiting the production of root and shoot dry biomass, whereas the root zone application of both Si and SiNP effectively mitigated oxidative and osmotic cell damage. Compared to Si, SiNP yielded better efficiency by improving the activity of enzymatic antioxidants [including superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT)], non-enzymatic antioxidants [ascorbic acid (AsA) and glutathione contents], and accumulation of organic osmolytes [proline and glycinebetaine (GB)]. SiNP also regulated the nutrient profile of the plants by increasing N, P, K, and Zn contents while limiting Mn and Fe concentration to a less toxic level. A negative correlation between antioxidant activities and lipid peroxidation rates was observed in SiNP-treated plants under hypoxia stress. Conclusively, SiNP-treated plants combat hypoxia more efficiently stress than conventional Si by boosting antioxidant activities, osmoprotectant accumulation, and micronutrient regulation.

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The initiation of bud burst in grapevine features dynamic regulation of the apoplastic pore size

Abstract: Molecular modelling, histochemistry, and micro-computed tomography reveal that the apoplastic pore size is dynamically regulated during bud burst in grapevine, and associated with oxygenation of the meristematic core.

Cited by 24 publications

References 39 publications

The bud dormancy disconnect: latent buds of grapevine are dormant during summer despite a high metabolic rate

The bud dormancy disconnect: latent buds of grapevine are dormant during summer despite a high metabolic rate

Soil Water Content Directly Affects Bud Burst Rate in Single-Node Cuttings of Perennial Plants

Silicon Alleviate Hypoxia Stress by Improving Enzymatic and Non-enzymatic Antioxidants and Regulating Nutrient Uptake in Muscadine Grape (Muscadinia rotundifolia Michx.)

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