The initial reactions of fatty acid biosynthesis in plants

Jaworski, Jan G.; Tai, Heeyoung; Ohlrogge, John B.; Post-Beittenmiller, Dusty

doi:10.1016/0163-7827(94)90008-6

Cited by 16 publications

(17 citation statements)

References 13 publications

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“…Whilst we have previously observed proteolysis of FAS components in oil seed rape this does not seem to a problem in Avocado/~-keto reductase preparations [13]. If KAS III does have an intrinsic ACAT activity, as has been reported by other workers [6] the enzyme reaction can be seen to have two components (Fig. 1).…”

Section: Discussionsupporting

confidence: 51%

“…Jaworski and co-workers [6] working on thiolactomycin inhibition in spinach reported that they initially obtained inconsistent results, but observations made using thiolactomycin prepared under a variety of conditions allowed them to propose that KAS III is inhibited by an oxidative breakdown product of thiolactomycin. We have repeated their work, paying appropriate attention to the antibiotic preparation [6] and show here a clear inhibition of KAS III and ACAT activities. We found that thiolactomycin taken up directly into buffer is not inhibitory to either ACAT or KAS III confirming the work of Jaworski [6].…”

Section: Discussionmentioning

confidence: 99%

“…Small quantities were dissolved in methanol and the required amount aliquoted into the assay tube, dried by vacuum centrifugation in a Jouan vacuum drier, RC 1022, and then incubated at room temperature for 10 min allowing the antibiotic to undergo the necessary degradation prior to the addition of enzyme [6]. Cerulenin was dissolved in methanol, aliquoted and dried in the same way.…”

Section: Antibiotic Preparationmentioning

confidence: 99%

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Acetoacyl-acyl carrier protein synthase from avocado: its purification, characterisation and clear resolution from acetyl CoA:ACP transacylase

Gulliver

Slabas

1994

Plant Mol Biol

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beta-ketoacyl-ACP synthetase III (KAS III) has been purified from avocado using a six-step purification procedure. The enzyme, which is cerulenin-insensitive and thiolactomycin-sensitive, was assayed using a partial component reaction: acetyl CoA:ACP transacylase (ACAT) activity. KAS III activity is distinguished from ACAT activity on the basis that the former is highly stimulated by the addition of malonyl CoA in the presence of malonyl-CoA:ACP transacylase, and the latter is not. KAS III and ACAT activity have been separated from each other thus providing the first evidence that these two discrete activities exist in higher plants. Both of these enzymes have been implicated in the initial reactions of fatty acid synthesis. KAS III was purified 134-fold using a combination of PEG precipitation, Fast Q, ammonium sulphate precipitation, Phenyl Sepharose and ACP-affinity chromatography. The enzyme requires Triton X-100 for solubility and is highly salt sensitive. The subunit molecular mass of 37 kDa has been identified by SDS-PAGE. The results of gel filtration analysis are consistent with the native enzyme being homodimeric. The native molecular mass of KAS III is 69 kDa and that of ACAT 18.5 kDa. The enzyme has a pH optimum of 7.0-7.5, which is similar to the pH optimum of the ACAT reaction. The Km for acetyl CoA is 12.5 microM and the Km for malonyl-ACP is 14 microM. Both KAS III and ACAT are sensitive to thiolactomycin inhibition. The results are discussed with respect to the potential role of acetyl CoA:ACP transacylase in plants.

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Section: Discussionsupporting

confidence: 51%

Section: Discussionmentioning

confidence: 99%

Section: Antibiotic Preparationmentioning

confidence: 99%

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Acetoacyl-acyl carrier protein synthase from avocado: its purification, characterisation and clear resolution from acetyl CoA:ACP transacylase

Gulliver

Slabas

1994

Plant Mol Biol

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“…In general, constitutive FA synthesis results in the production of longchain FAs (ϾC 12 ). In plants and bacteria, the proportion of different length FAs generated by the type II FAS complex is controlled by the activities of the ketosynthase and acyl-acyl carrier protein thioesterase domains (32). The mechanism regulating FA chain length for the mammalian type I FAS is not clear but it can involve tissue specific control by a distinct thioesterase activity (33).…”

Section: Discussionmentioning

confidence: 99%

“…Standard molecular techniques were used to manipulate DNA in vitro (14). DNA probes were generated by labeling fragments with 32 P by random primer extension as described by Sambrook et al (14).…”

Section: Methodsmentioning

confidence: 99%

Aspergillus has distinct fatty acid synthases for primary and secondary metabolism

Brown

Adams

Keller

1996

Proc. Natl. Acad. Sci. U.S.A.

141

103

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Aspergillus nidulans contains two functionally distinct fatty acid synthases (FASs): one required for primary fatty acid metabolism (FAS) and the other required for secondary metabolism (sFAS). FAS mutants require longchain fatty acids for growth, whereas sFAS mutants grow normally but cannot synthesize sterigmatocystin (ST), a carcinogenic secondary metabolite structurally and biosynthetically related to af latoxin. sFAS mutants regain the ability to synthesize ST when provided with hexanoic acid, supporting the model that the ST polyketide synthase uses this shortchain fatty acid as a starter unit. The characterization of both the polyketide synthase and FAS may provide novel means for modifying secondary metabolites.

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Multienzyme Complexes Involved in the Benson–Calvin Cycle and in Fatty Acid Metabolism

Gontero

Lebreton

Graciet

2018

Annual Plant Reviews Online

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The initial reactions of fatty acid biosynthesis in plants

Cited by 16 publications

References 13 publications

Acetoacyl-acyl carrier protein synthase from avocado: its purification, characterisation and clear resolution from acetyl CoA:ACP transacylase

Acetoacyl-acyl carrier protein synthase from avocado: its purification, characterisation and clear resolution from acetyl CoA:ACP transacylase

Aspergillus has distinct fatty acid synthases for primary and secondary metabolism

Multienzyme Complexes Involved in the Benson–Calvin Cycle and in Fatty Acid Metabolism

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