The initial fertilizing capacity of longerm-stored liquid boar semen following pre- and postovulatory insemination

Waberski, Dagmar; Weitze, K. F.; Lietmann, C.; Lage, W. Lübbert zur; Bortolozzo, Fernando Pandolfo; Willmen, T.; Petzoldt, R

doi:10.1016/0093-691x(94)90188-o

Cited by 53 publications

(50 citation statements)

References 16 publications

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“…During this time, standard semen parameters, i.e., motility and plasma membrane integrity, usually give no indication of a substantial reduction in semen quality; both these features are considered to be minimum prerequisites for satisfactory fertility outcomes after insemination (1). However, results from an insemination trial under experimental conditions have suggested that, despite relatively high values for standard semen parameters, storage in vitro reduces the ability of sperm to survive and fertilize in vivo (2); the study even demonstrated that semen storage for as little as 24-48 h in BTS extender leads to a significant reduction in the fertilization rate of ova in vivo. It is, therefore, clear that better laboratory methods for detecting changes in sperm fertilizing capability are required.…”

Section: Introductionmentioning

confidence: 99%

Bivalent response to long‐term storage in liquid‐preserved boar semen: A flow cytometric analysis

Henning

Petrunkina

Harrison³

et al. 2012

Cytometry Pt A

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The fertility of liquid-preserved boar semen declines during storage at 178C, insemination trials even indicating early losses in fertilizing ability within the first 24-48 h of storage. Standard semen parameters barely reflect these changes in semen quality, and new approaches for assessment of functional changes in stored spermatozoa are needed. Capacitation, the essential prefertilization step for spermatozoa in the female genital tract, is specifically induced in vitro by bicarbonate. Therefore, we have investigated changes in responsiveness of boar spermatozoa to bicarbonate during storage. Ejaculates of 14 boars were diluted in Beltsville thawing solution, cooled to 178C and stored for 12, 24, 72, 120, and 168 h before investigation. At each time, basic semen quality was characterized by sperm motility and viability. Subsequently, washed subsamples were incubated in variants of an in vitro fertilization (IVF) medium and assessed for kinetic changes of viability (plasma membrane integrity) and intracellular calcium concentration using flow cytometry in combination with propidium iodide and Fluo-3. By this means, it was possible to determine specific effects of bicarbonate and calcium on sperm subpopulations over incubation time. During storage, standard semen parameters remained on a high level. However, flow cytometric analysis of sperm responses to capacitating and control media revealed two opposing effects of storage. There was a loss of response to bicarbonate in part of the live sperm population but an increasing degree of instability in the rest. Assessment of response to capacitating media by flow cytometry appears a markedly more sensitive way of monitoring sperm functionality during storage than the standard semen parameters of motility and viability. ' 2012 International Society for Advancement of Cytometry

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Section: Introductionmentioning

confidence: 99%

Bivalent response to long‐term storage in liquid‐preserved boar semen: A flow cytometric analysis

Henning

Petrunkina

Harrison³

et al. 2012

Cytometry Pt A

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“…It is likely that the binding differences observed here indicate differences in fertilisation capacity. Whether this is ultimately reflected in reduced farrowing rate and litter sizes depends largely on the insemination dose, intervals between AI and ovulation and other farm management factors (Waberski et al 1994a, Johnson et al 2000. The present study was not designed to find a correlation between BI and fertility, and it cannot be expected that there would be a linear relationship for any sperm parameter to be tested.…”

Section: Sperm Parameter Controlmentioning

confidence: 99%

“…At present, there is no satisfactory explanation for the reduced fertility of stored boar semen. Within the first 3 days of semen preservation, the motility and acrosome integrity of sperm usually remain high, but various insemination trials indicate that the sperm suffer functional damage from storage conditions (Waberski et al 1994a, Johnson et al 2000. Sperm-oviduct binding assays have been used in various species, mostly to study the physiology of sperm-oviduct interaction and to determine the criteria for sperm selection.…”

Section: Introductionmentioning

confidence: 99%

“…In the pig, sperm fertilising competence in the oviduct is maintained for up to 24 h. However, it has been shown in vivo that the number of fertilising competent sperm in the oviduct is lowered by deficiencies in semen quality; these can be due either to individual characteristics of the boar or to ageing of the sperm during storage prior to insemination. More specifically, fewer viable sperm reach the oviductal isthmus, and viable sperm with decreased membrane stability have a shorter life span in the oviduct (Saacke et al 1994, Waberski et al 1994a). At present, there is no satisfactory explanation for the reduced fertility of stored boar semen.…”

Section: Introductionmentioning

confidence: 99%

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Binding of boar spermatozoa to oviductal epithelium in vitro in relation to sperm morphology and storage time

Waberski¹,

Magnus²,

Ardón³

et al. 2006

Reproduction

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In vitro short-term storage of boar semen for up to 72 h before insemination negatively affects fertility, but this often remains undetected during semen quality assessment. One important sperm function is the ability to form the functional sperm reservoir in the oviduct. In the present study, we used the modified oviductal explant assay to study sperm binding to oviductal epithelium in vitro in diluted boar semen stored for 24 or 72 h. First, we determined the kinetics of in vitro sperm binding to oviductal epithelium in relation to co-incubation time of sperm and oviductal tissue pieces. Then, we studied how the binding of sperm to oviductal epithelium was affected by in vitro semen storage and by differences among individual boars. Sperm binding after different incubation times was significantly higher when semen was stored 24 h than after 72-h storage (P < 0.05), and peaked at 30-90 min of incubation. Sperm binding differed between boars (n 5 44), and was negatively correlated to the percentage of sperm with cytoplasmic droplets (R 5 20.51, P < 0.001). There were no significant changes in motility, acrosome integrity and propidium iodide stainability during the 72-h storage period. However, sperm-binding indices were significantly lower after 72 h in vitro storage than after 24-h storage in sperm from boars with normal semen quality (P < 0.05); in contrast, the binding capacity of sperm from boars with higher percentages of morphologically altered sperm remained at a low level. The spermbinding capacity of sperm from four of the five boars with known subfertility was lower than the mean binding index minus one standard deviation of the boar population studied here. It is concluded that changes in the plasma membrane associated with in vitro ageing reduce the ability of stored boar sperm to bind to the oviductal epithelium. This study shows the potential of sperm-oviduct binding as a tool to assess both male fertility and changes in sperm function associated with in vitro ageing.

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“…Resultados semelhantes aos deste estudo, em relação ao BTS, foram observados por WABERSKI et al (1994) e ALEXOPOULOS et al (1996, com sêmen conservado de dois a cinco dias e doses inseminantes de um a cinco bilhões de espermatozóides.…”

Section: Conclusõesunclassified

Efeito de sêmen resfriado e diluído em Beltsville Thawing Solution, Zorlesco-modificado e BTZOR no desempenho reprodutivo de fêmeas suínas inseminadas

Vasconcelos¹,

Moraes²,

Rigolon

et al. 2001

R. Bras. Zootec.

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RESUMO -Utilizaram-se 83 fêmeas, inseminadas com ejaculados de três cachaços, diluídos em Beltsville Thawing Solution (BTS), Zorlesco-modificado (ZOR) e BTZOR (meio desenvolvido na Universidade Estadual de Maringá), sendo 28 fêmeas inseminadas com o diluente BTS, 28 com ZOR e 27 com BTZOR, com o objetivo de estudar os índices de retorno ao cio e de parição e os números médios de leitões nascidos e nascidos vivos das fêmeas inseminadas. As fêmeas inseminadas com ZOR apresentaram melhor índice de parição (92,9%), quando comparadas às inseminadas com BTZOR (70,4%) e BTS (67,9%). O índice de retorno ao cio foi menor nas fêmeas inseminadas com ZOR (7,14%) em comparação às inseminadas com BTZOR (29,6%) e BTS (32,14%), não tendo diferença entre o BTS e o BTZOR. Concluiu-se que os melhores resultados quanto ao desempenho reprodutivo das fêmeas ocorreram nas inseminações realizadas com sêmen diluído em Zorlesco-modificado.Palavras-chave: desempenho reprodutivo, diluentes, fêmeas suínas, inseminação artificial Reproductive Performance of Swine Females Inseminated with Cooled and Diluted Semen In Beltsville Thawing Solution, Modified-Zorlesco and BTZORABSTRACT -Eighty-three swine females were inseminated with semen from three boars, diluted in Beltsville Thawing Solution (BTS), Modified-Zorlesco (ZOR) and BTZOR (a medium developed at Universidade Estadual de Maringá). Twenty eight females were inseminated with BTS, 28 with ZOR and 27 with BTZOR, with the objective to evaluate the indexes of heat return and farrowing rate, and the averages numbers of born piglets and born alive piglets from the inseminated females. The females inseminated with ZOR showed the best farrowing rate index (92.9%) when compared to those inseminated with BTZOR (70,4%) and with BTS (67.9%). The lowest heat return rate was from inseminated females with ZOR (7.14%) when compared to BTZOR (29.6%) and BTS (32.14%), and there was no difference between BTS and BTZOR. The best reproductive performance results were observed for artificial inseminations with semen diluted in Modified-Zorlesco diluents. Rev. bras. zootec., 30(2):402-408, 2001Introdução A busca por um diluente que proporcione bons resultados na inseminação artificial (IA) de suínos tem sido uma constante dos pesquisadores desta área. BARITEAU et al. (1977) afirmaram que o uso do sêmen diluído na IA simplifica as operações desta técnica e não diminui os resultados de fecundidade média, o que também está de acordo com ZAVOS e LIPTRAP (1987), que observaram fecundidade e leitegadas iguais ou melhores que as obtidas por monta natural ou com sêmen congelado (SCHEID et al. 1986). SENEGACNIK (1976), testando a resposta de sêmen resfriado em 2000 porcas, verificou que o índice de concepção para uma ou duas inseminações, com sêmen conservado de 1 a 24 horas, variou de 66,12 a 70,50% para vários diluentes. JOHNSON et al. (1979), ao realizarem inseminação com sêmen resfriado e diluído em Kiev, no primeiro dia de colheita do sêmen, obtiveram 79% de parição, com a média de 10,6 leitões por leitegada. GOT...

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The initial fertilizing capacity of longerm-stored liquid boar semen following pre- and postovulatory insemination

Cited by 53 publications

References 16 publications

Bivalent response to long‐term storage in liquid‐preserved boar semen: A flow cytometric analysis

Bivalent response to long‐term storage in liquid‐preserved boar semen: A flow cytometric analysis

Binding of boar spermatozoa to oviductal epithelium in vitro in relation to sperm morphology and storage time

Efeito de sêmen resfriado e diluído em Beltsville Thawing Solution, Zorlesco-modificado e BTZOR no desempenho reprodutivo de fêmeas suínas inseminadas

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