The inhibitory mechanism of gap junctional intercellular communication induced by polyethylene and the restorative effects by surface modification with various proteins

Nakaoka, Ryusuke; Tsuchiya, Toshie; Nakamura, Akitada

doi:10.1002/1097-4636(20011215)57:4<567::aid-jbm1203>3.0.co;2-d

Cited by 8 publications

(8 citation statements)

References 11 publications

(24 reference statements)

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“…and intercellular communication, compared with laminin-rich Matrigel (22). Numerous other studies also have shown that the ECM can directly and indirectly alter gap junctional communication in various cell types (5,24,25). These results, in combination with our own, strongly suggest that the ECM can influence both the expression and function of gap junctions.…”

Section: Discussionsupporting

confidence: 86%

RGD and YIGSR synthetic peptides facilitate cellular adhesion identical to that of laminin and fibronectin but alter the physiology of neonatal cardiac myocytes

Boateng

Lateef

Mosley

et al. 2005

American Journal of Physiology-Cell Physiology

117

101

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In the mammalian heart, the extracellular matrix plays an important role in regulating cell behavior and adaptation to mechanical stress. In cell culture, a significant number of cells detach in response to mechanical stimulation, limiting the scope of such studies. We describe a method to adhere the synthetic peptides RGD (fibronectin) and YIGSR (laminin) onto silicone for culturing primary cardiac cells and studying responses to mechanical stimulation. We first examined cardiac cells on stationary surfaces and observed the same degree of cellular adhesion to the synthetic peptides as their respective native proteins. However, the number of striated myocytes on the peptide surfaces was significantly reduced. Focal adhesion kinase (FAK) protein was reduced by 50% in cardiac cells cultured on YIGSR peptide compared with laminin, even though beta(1)-integrin was unchanged. Connexin43 phosphorylation increased in cells adhered to RGD and YIGSR peptides. We then subjected the cardiac cells to cyclic strain at 20% maximum strain (1 Hz) for 48 h. After this period, cell attachment on laminin was reduced to approximately 50% compared with the unstretched condition. However, in cells cultured on the synthetic peptides, there was no significant difference in cell adherence after stretch. On YIGSR peptide, myosin protein was decreased by 50% after mechanical stimulation. However, total myosin was unchanged in cells stretched on laminin. These results suggest that RGD and YIGSR peptides promote the same degree of cellular adhesion as their native proteins; however, they are unable to promote the signaling required for normal FAK expression and complete sarcomere formation in cardiac myocytes.

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Section: Discussionsupporting

confidence: 86%

RGD and YIGSR synthetic peptides facilitate cellular adhesion identical to that of laminin and fibronectin but alter the physiology of neonatal cardiac myocytes

Boateng

Lateef

Mosley

et al. 2005

American Journal of Physiology-Cell Physiology

117

101

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“…NHOst cultured on SAM‐covered coverslips were subjected to fluorescence recovery after photobleaching (FRAP) analysis to evaluate the effect of these surfaces on the GJIC. FRAP analysis was carried out according to the procedure of Wade et al26 with some modifications 20. Briefly, NHOst were plated and incubated for 1 day on the SAM‐covered coverslips.…”

Section: Methodsmentioning

confidence: 99%

Effects of surface chemistry prepared by self‐assembled monolayers on osteoblast behavior

Nakaoka

Yamakoshi

Isama

et al. 2010

J Biomedical Materials Res

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A surface of biomaterials is known to affect the behavior of cells after their adhesion on the surface, indicating that surface characteristics of biomaterials play an important role in cell adhesion, proliferation, and differentiation. To assess the effects of functional groups on biomaterial surface, normal human osteoblasts (NHOsts) were cultured on surfaces coated with self-assembled monolayers (SAMs) containing various functional groups, and the adhesion, proliferation, differentiation, and gap junctional intercellular communication (GJIC) of the NHOsts were investigated. In the case of SAM with terminal methyl groups (hydrophobic surface), NHOst adhesion and proliferation was less prevalent. In contrast, NHOsts were adhered well on SAMs with hydroxyl, carboxyl, amino, phosphate, and sulfate group, which are relatively hydrophilic, their proliferation and differentiation level were dependent on the type of functional groups. Especially, when they were cultured on either SAMs with phosphate or sulfate group, both their alkaline phosphate activity and the calcium deposition by them were enhanced more than those cultured on a collagen-coated dish. More interestingly, GJIC of NHOsts, which has been reported to play a role in cell differentiation as well as homeostasis of cells, were not significantly different among the SAM surfaces tested. These suggest that a specific functional group on a material surface can regulate NHOst adhesion, proliferation, and differentiation via cell-functional group interaction without influencing their homeostasis.

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“…It suggests that inhibitory action of PS microspheres is not due to the change in the connexin status. From our previous study, 24) however, the expression of connexin 43 in fibroblasts cultured on a PE film was apparently different from that on a normal culture dish, suggesting that an interaction between the cell and PE surface resulted in irregular intracellular signal transduction, followed by abnormal expression of connexin. It was reported that the phosphorylated state of connexin 43 was important for its accurate assembly and distribution inside the cell.…”

Section: Discussionmentioning

confidence: 82%

Biocompatibility of Various Kinds of Polymer Microspheres Estimated from Their Effect on Gap Junctional Intercellular Communication of Fibroblasts

Nakaoka¹,

Tsuchiya²

2002

Mater. Trans.

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Gap junctional intercellular communication is a function that plays an important role in maintaining cell and tissue homeostasis and in regulating cell growth, development and differentiation. Change in this function when contacting fibroblasts with various polymer microspheres was estimated using the fluorescence recovery after photobleaching (FRAP) assay system. When the cells were in contact on test dishes, the inhibition level increased as the diameters of polystyrene microspheres decreased, except for a microsphere with 0.5 µm diameter. The function was inclined to be recovered with the increase of the incubation time, while it was not recovered when the cells were cultured with pre-coated polystyrene microspheres. As well as inhibitory activities of the function, cytotoxicity potentials of tested microspheres depended on their diameter and their composition. These findings suggest that the size and the physico-chemical character of polymer microspheres, and how cells recognize them plays important roles in causing influences of the microspheres on both gap junctional intercellular communication and their cytotoxicity. Therefore, estimating the inhibitory effect of biomaterials on the gap junctional intercellular communication will provide valuable information about the biocompatibility of materials even in the form of particles.

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The inhibitory mechanism of gap junctional intercellular communication induced by polyethylene and the restorative effects by surface modification with various proteins

Cited by 8 publications

References 11 publications

RGD and YIGSR synthetic peptides facilitate cellular adhesion identical to that of laminin and fibronectin but alter the physiology of neonatal cardiac myocytes

RGD and YIGSR synthetic peptides facilitate cellular adhesion identical to that of laminin and fibronectin but alter the physiology of neonatal cardiac myocytes

Effects of surface chemistry prepared by self‐assembled monolayers on osteoblast behavior

Biocompatibility of Various Kinds of Polymer Microspheres Estimated from Their Effect on Gap Junctional Intercellular Communication of Fibroblasts

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