The inhibitory effect of superparamagnetic iron oxide nanoparticle (Ferucarbotran) on osteogenic differentiation and its signaling mechanism in human mesenchymal stem cells

Chen, Ying‐Chun; Hsiao, Jong‐Kai; Liu, Hon‐Man; Lai, I-Yin; Yao, Ming; Hsu, Szu-Chun; Ko, Bor-Sheng; Chen, Yao‐Chang; Yang, Ching‐Fen; Huang, Dong-Ming

doi:10.1016/j.taap.2010.03.011

Cited by 145 publications

(113 citation statements)

References 33 publications

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“…It has been demonstrated by several groups that SPIO particles have little toxicity and few side effects on cell proliferation and activity (21,22). However, certain study results have indicated the inhibitory effect of SPIO particles on MSC differentiation and its signaling mechanism (23). To conclude, the effect of SPIO upon labeled MSCs on a cellular level remains uncertain.…”

Section: Discussionmentioning

confidence: 98%

Assessment of biological characteristics of mesenchymal stem cells labeled with superparamagnetic iron oxide particles in vitro

et al. 2011

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Abstract. Mesenchymal stem cell (MSC) transplantation provides a novel strategy for the treatment of human disease. MR imaging (MRI) is able to track transplanted stem cells labeled with superparamagnetic iron oxide (SPIO) in vivo. However, the effect of SPIO upon labeled MSCs remains unclear on a cellular level. In this study, the biological characteristics of rat MSCs labeled with home-synthesized SPIO particles were evaluated. The MSCs were isolated from the bone marrow of 5 adult Sprague-Dawley rats and labeled with home-synthesized SPIO particles at a final iron concentration of 20 µg/ml. Labeled MSCs were confirmed with Prussian blue staining and transmission electron microscopy. The quantity of iron per cell was determined by atomic absorption spectrometry. Cell viability, proliferation, membranous antigen and multiple differentiation ability were compared between labeled and unlabeled MSCs. The rat MSCs were effectively labeled and the labeling efficiency was approximately 100%, as revealed by Prussian blue staining. The SPIO particles located in the endosomal vesicles of the MSCs were confirmed by transmission electron microscopy. No significant differences were observed in cell viability, proliferation, membranous antigen and multiple differentiation ability between the labeled and unlabeled MSCs (P>0.05). In conclusion, MSCs are able to be effectively labeled by home-synthesized SPIO particles without influencing their main biological characteristics. IntroductionAs a potential interventional procedure, stem cell transplantation provides a new strategy for the treatment of incurable human diseases and organ failure (1-3). The plasticity of bone marrow mesenchymal stem cells (MSCs) has been intensively investigated, since this approach generates less ethical and social controversies. The use of bone marrow MSCs also offers several advantages, including ease of collection and rapid in vivo and in vitro repopulation. Numerous studies have shown that MSCs are capable of forming functional components of organ tissues, including the kidney, heart and liver (1-4).A non-invasive in vivo technique that permits evaluation of the efficiency of transplantation and the potential migration of transplanted cells would prove to be an essential tool for treatment assessment. To better understand the mechanisms of the cell therapy, in vivo monitoring of the cellular dynamics of the transplanted MSCs has been proposed. Recently, MR imaging (MRI) has proven to be effective in tracking the distribution of transplanted MSCs in vivo by way of labeling cells with superparamagnetic iron oxide (SPIO) particles (5-7).However, it is not clear whether the SPIO labeling technique of MSCs is safe. Additionally, the effect of SPIO upon labeled cells remains unclear on a cellular level (8). Thus, the purpose of the present study was to investigate whether and how the labeling of MSCs with SPIO affects the biological characteristics of MSCs. Materials and methodsCell culture. This study was approved by our institutional Animal Use and...

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Section: Discussionmentioning

confidence: 98%

Assessment of biological characteristics of mesenchymal stem cells labeled with superparamagnetic iron oxide particles in vitro

et al. 2011

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“…Due to the partially complicated labeling protocols, simplified strategies using clinically approved materials (e. g. Endorem ® /Feridex ® , Superfect ® , Lipofectamine Plus ® , poly-Llysine (PLL), protamine) and low cell toxicity have been described in recent years [148,149]. While most studies do not describe any significant changes in cell behavior (e. g. apoptosis rate, proliferation index, differentiation behavior) [150,151], individual studies show changes in migration behavior and the ability to form colonies [152], in cell vitality [153], in differentiation behavior [154], or in the expression pattern [152]. By the same token, the influence of cell labeling on the long-term behavior of cells and the course of disease is currently unclear [155] and must be studied more closely in the future [156].…”

Section: Cell Labeling and Cell Imaging In Mrimentioning

confidence: 99%

Superparamagnetic Iron Oxide Nanoparticles in Biomedicine: Applications and Developments in Diagnostics and Therapy

Ittrich¹,

Peldschus²,

Raabe³

et al. 2013

Fortschr Röntgenstr

124

101

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Superparamagnetic iron oxide nanoparticles (SPIO) can be used to image physiological processes and anatomical, cellular and molecular changes in diseases. The clinical applications range from the imaging of tumors and metastases in the liver, spleen and bone marrow, the imaging of lymph nodes and the CNS, MRA and perfusion imaging to atherosclerotic plaque and thrombosis imaging. New experimental approaches in molecular imaging describe undirected SPIO trapping (passive targeting) in inflammation, tumors and associated macrophages as well as the directed accumulation of SPIO ligands (active targeting) in tumor endothelia and tumor cells, areas of apoptosis, infarction, inflammation and degeneration in cardiovascular and neurological diseases, in atherosclerotic plaques or thrombi. The labeling of stem or immune cells allows the visualization of cell therapies or transplant rejections. The coupling of SPIO to ligands, radio- and/or chemotherapeutics, embedding in carrier systems or activatable smart sensor probes and their externally controlled focusing (physical targeting) enable molecular tumor therapies or the imaging of metabolic and enzymatic processes. Monodisperse SPIO with defined physicochemical and pharmacodynamic properties may improve SPIO-based MRI in the future and as targeted probes in diagnostic magnetic resonance (DMR) using chip-based ?NMR may significantly expand the spectrum of in vitro analysis methods for biomarker, pathogens and tumor cells. Magnetic particle imaging (MPI) as a new imaging modality offers new applications for SPIO in cardiovascular, oncological, cellular and molecular diagnostics and therapy. Key Points: Citation Format:

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“…96 Divergent results have been obtained concerning osteogenic and chondrogenic differentiation. [94][95][96][97] We were able to obtain chondrogenic, osteogenic, and adipogenic differentiation of mesenchymal stem cells after SPION labeling. 15 More recently, Balakumaran et al 98 successfully demonstrated the potential of SPI-ON-labeled stem cells to appropriately differentiate in vivo.…”

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confidence: 99%

Tracking stem cells with superparamagnetic iron oxide nanoparticles: perspectives and considerations

Jasmin¹,

Souza²,

Louzada³

et al. 2017

IJN

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Superparamagnetic iron oxide nanoparticles (SPIONs) have been used for diagnoses in biomedical applications, due to their unique properties and their apparent safety for humans. In general, SPIONs do not seem to produce cell damage, although their long-term in vivo effects continue to be investigated. The possibility of efficiently labeling cells with these magnetic nanoparticles has stimulated their use to noninvasively track cells by magnetic resonance imaging after transplantation. SPIONs are attracting increasing attention and are one of the preferred methods for cell labeling and tracking in preclinical and clinical studies. For clinical protocol approval of magnetic-labeled cell tracking, it is essential to expand our knowledge of the time course of SPIONs after cell incorporation and transplantation. This review focuses on the recent advances in tracking SPION-labeled stem cells, analyzing the possibilities and limitations of their use, not only focusing on myocardial infarction but also discussing other models.

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The inhibitory effect of superparamagnetic iron oxide nanoparticle (Ferucarbotran) on osteogenic differentiation and its signaling mechanism in human mesenchymal stem cells

Cited by 145 publications

References 33 publications

Assessment of biological characteristics of mesenchymal stem cells labeled with superparamagnetic iron oxide particles in vitro

Assessment of biological characteristics of mesenchymal stem cells labeled with superparamagnetic iron oxide particles in vitro

Superparamagnetic Iron Oxide Nanoparticles in Biomedicine: Applications and Developments in Diagnostics and Therapy

Tracking stem cells with superparamagnetic iron oxide nanoparticles: perspectives and considerations

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