The Inhibitory Effect of Nodakenin on Mast‐Cell‐Mediated Allergic Inflammation Via Downregulation of NF‐κB and Caspase‐1 Activation

Abstract

Nodakenin, a coumarin isolated from the roots of Angelicae gigas, is effective for treating function control disorders, bacterial infections, pain, diarrhea, vitamin E deficiency, and for relaxation of the uterus. The aim of this study was to investigate the antiallergic related inflammatory effects in phorbol-12-myristate 13-acetate plus calcium ionophore A23187 (PMACI)-stimulated human mast cells (HMC-1) or anaphylactic activity in a mouse model. Nodakenin inhibited the mRNA expression and production of pro-inflammatory cytokines such as tumor necrosis factor (TNF)-α, interleukin (IL)-6, and IL-1β in PMACI-stimulated HMC-1. We also studied the inhibitory effects of nodakenin on the nuclear translocation of nuclear factor kappa B (NF-κB) and activation of caspase-1, inhibitory κB kinase (IKK), and Akt in PMACI-stimulated HMC-1. However, mitogen-activated protein kinase (MAPK) activation was not sufficient to abrogate the stimulus. In addition, administration of nodakenin at 20 mg/kg inhibited histamine release and protected mice against compound 48/80-induced anaphylactic mortality. Furthermore, Nodakenin inhibited the mRNA expression and production of pro-inflammatory cytokines and caspase-1 activation in compound 48/80-induced anaphylactic mice. These results suggest new insight that nodakenin may be a promising antiallergic related inflammatory agent for inflammatory disorders. J. Cell. Biochem. 118: 3993-4001, 2017. © 2017 Wiley Periodicals, Inc.

Nodakenin alleviated obstructive nephropathy through blunting Snail1 induced fibrosis

“…Nodakenin, a small‐molecule coumarin isolated from the roots of Angelicae gigas, has been reported to posess multiple biological activities such as alleviating inflammation, enhancing cognitive function and so on 12,13 . However, whether nodakenin possesses the anti‐fibrotic actions remains poorly unclear.…”

“…In recent years, drugs isolated from the medicinal herbal have drawn increasing attractions from the scientific researchers as an alternative therapy for the prevention and treatment of various renal disease 8‐11 . Nodakenin, a coumarin derived from the roots of Angelicae gigas , has been reported to treat against multiple diseases including alleviating inflammation to treat against lethal endotoxin shock and allergic inflammation, enhancing cognitive function and adult hippocampal neurogenesis and so on 12‐14 . However, little reports have been suggested the effect of nodakenin against renal fibrosis.…”

Oral Wound Healing Effects of Acai Berry Water Extracts in Rat Oral Mucosa

“…Mast cells play an important role in increasing inflammation by secreting histamine. Thus, decrease of mast cells can help improve wound healing by decreasing inflammation ( 24 ). In the present study, the group treated with ABWE displayed fastest regeneration of tissue.…”

A network pharmacology approach to determine the synergetic mechanisms of herb couple for treating rheumatic arthritis

“… 20 Moreover, nodakenin (OB=57.12, DL=0.69) plays an important therapeutic effect in inflammatory disorders and has been regarded as one of the standard ingredients of NI in Chinese Pharmacopoeia. 10 , 21 …”