The inhibitory effect of nitrite, a stable product of nitric oxide (NO) formation, on arginase

Hrabák, A.; Bajor, Tamás; Temesi, A; Mészáros, György

doi:10.1016/0014-5793(96)00659-x

Cited by 45 publications

(27 citation statements)

References 30 publications

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“…Recent studies have reported that arginase modulates NOS activity via arginine consumption (17,34). On the other hand, it has been reported that arginase is inhibited by the nitrite, a stable end product of NO (35). We demonstrated a negative correlation between seminal plasma NO level and arginase activity during stress.…”

Section: Discussionsupporting

confidence: 53%

Effect of psychological stress on the L-arginine-nitric oxide pathway and semen quality

Eskiocak

Gözen

Taşkıran

et al. 2006

Braz J Med Biol Res

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It has been reported that mental stress causes abnormality of spermiogram parameters. We investigated the effect of psychological stress on the L-arginine-nitric oxide (NO) pathway. Semen samples were collected from 29 healthy fourth semester medical students just before (stress) and 3 months after (non-stress) the final examinations. Psychological stress was measured by the State Anxiety Inventory questionnaire. After standard semen analysis, arginase activity and NO concentration were measured spectrophotometrically in the seminal plasma. Measurements were made in duplicate. During the stress period, sperm concentration (41.28 ± 3.70 vs 77.62 ± 7.13 x 10 6 /mL), rapid progressive motility of spermatozoa (8.79 ± 1.66 vs 20.86 ± 1.63%) and seminal plasma arginase activity (0.12 ± 0.01 vs 0.22 ± 0.01 U/mL) were significantly lower than in the non-stress situation, whereas seminal plasma NO (17.28 ± 0.56 vs 10.02 ± 0.49 µmol/L) was higher compared to the non-stress period (P < 0.001 for all). During stress there was a negative correlation between NO concentration and sperm concentration, the percentage of rapid progressive motility and arginase activity (r = -0.622, P < 0.01; r = -0.425, P < 0.05 and r = -0.445, P < 0.05, respectively). These results indicate that psychological stress causes an increase of NO level and a decrease of arginase activity in the L-arginine-NO pathway. Furthermore, poor sperm quality may be due to excessive production of NO under psychological stress. In the light of these results, we suggest that the arginine-NO pathway, together with arginase and NO synthase, are involved in semen quality under stress conditions. Correspondence

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Section: Discussionsupporting

confidence: 53%

Effect of psychological stress on the L-arginine-nitric oxide pathway and semen quality

Eskiocak

Gözen

Taşkıran

et al. 2006

Braz J Med Biol Res

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“…For example, in macrophages, high concentrations of NOS-derived NO feedback to switch off NOS activity [29]. The ornithine product spermine, can inhibit NOS activity [30] and nitrite, the stable metabolite of NO, can suppress arginase activity [31]. Therefore the activity of these two enzyme systems, under normal conditions, would be mutually exclusive.…”

Section: Discussionmentioning

confidence: 99%

The role of nitric oxide synthase isoforms and arginase in the pathogenesis of diabetic foot ulcers: possible modulatory effects by transforming growth factor beta 1

et al. 1999

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In diabetic foot ulcers (DFUs) the normal sequence of events in the healing process is disrupted. The condition is a major clinical concern, both in terms of cost ($500 million per year [1]), time and debility for the patient. It is the commonest cause of admission to hospital amongst diabetic patients [2] resulting in 50±70 % of all non-traumatic amputations [3] with a cost of at least £ 7000 per case in the United Kingdom [4]. Furthermore, with an increasingly aged population there is likely to be a rise in the incidence of these ulcers. It has therefore been the goal of clinicians for years to stimulate the normal reparative process in this debilitating disorder.The severe tissue destruction associated with DFUs suggests a number of candidate factors in their aetiology, in particular the metabolism of l-arginine is vital in the repair process. The metabolism of larginine by the enzyme nitric oxide synthase (NOS) Diabetologia (1999) AbstractAims/hypothesis. l-arginine, an amino acid involved in wound healing, is metabolised by one of two pathways; nitric oxide synthase and arginase. If metabolised by nitric oxide synthase, this can result in tissue destruction, or matrix deposition if metabolised by arginase. The aim therefore was to investigate the role of these enzymes in the pathogenesis of diabetic foot ulcers. Methods. The activity, proteins by Western blot analysis and cellular distribution (using immunocytochemistry) of these enzymes were measured in diabetic foot ulcers, diabetic skin and normal skin. Results. Total and inducible nitric oxide synthase (p < 0.001) and endothelial nitric oxide synthase were increased in diabetic ulcers compared with diabetic and normal skin and were associated with increased plasma nitrite concentrations in diabetic ulcers (p < 0.05). Inducible nitric oxide synthase was the major isoform, with the macrophage being the predominant cellular source. Similarly arginase activity was increased (p < 0.01) in diabetic ulcers. The protein levels corroborated with the activity data, with the fibroblast being the major cellular source. The spatial and cellular distribution of the two enzyme systems was distinct. Transforming growth factor-beta1 was decreased in diabetic ulcers in comparison with diabetic skin and normal skin. Conclusion/interpretation. Increased nitric oxide synthase activity in diabetic foot ulcers may be responsible for the impaired healing in this disease. Furthermore, the increased activity of arginase could account for the characteristic callus formation around these ulcers. In addition, the lower concentrations of transforming growth factor-beta1 in diabetic ulcers may explain the raised concentrations of nitric oxide in this condition. [Diabetologia (1999) 42: 748±757]

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“…To date there are no data, however, on the interaction and competition of these two alternate pathways and their effects on wound healing. It should be noted that L-hydroxy-arginine and nitrite, the intermediate and end products, respectively, of NO synthesis, are both strong arginase inhibitors (Hrabak et al, 1996). Furthermore, urea, the end product of arginase activity, inhibits NO formation (Okuno et al, 2004).…”

Section: Chemistry Of Nitric Oxidementioning

confidence: 99%

Nitric oxide in wound-healing

et al. 2005

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Modulation of the complex process of wound-healing remains a surgical challenge. Little improvement beyond controlling infection, gentle tissue handling, and debridement of necrotic tissue has been had in the modern era. However, increasing appreciation of the process from a biomolecular perspective offers the potential for making significant strides in wound modulation. The bioactive molecule nitric oxide was found to have wide-ranging impact on cellular activities, including the cellular responses engendered by wound healing. Current research suggests that nitric oxide and several nitric oxide donors can exert biologic effects, although the particular net responses of cells contributing to wound repair are context-dependent.

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The inhibitory effect of nitrite, a stable product of nitric oxide (NO) formation, on arginase

Cited by 45 publications

References 30 publications

Effect of psychological stress on the L-arginine-nitric oxide pathway and semen quality

Effect of psychological stress on the L-arginine-nitric oxide pathway and semen quality

The role of nitric oxide synthase isoforms and arginase in the pathogenesis of diabetic foot ulcers: possible modulatory effects by transforming growth factor beta 1

Nitric oxide in wound-healing

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