The Inhibitory Effect of Metronidazole and Doxycycline-HCl on proMMP-3 Production in Gingival Fibroblast

Kim, Hak-Joo; Lim, Ki-Jung; Kim, Sang-Mok; Kim, Byung-Ock; Han, Kyung-Yoon

doi:10.5051/jkape.2000.30.2.335

Cited by 2 publications

(4 citation statements)

References 2 publications

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“…Chlorhexidine inhibits MMP2, MMP8 and MMP9, 90 while metronidazole decreases the activity of proMMP-3. 91 Frankova et al reported that 10 nm AgNPs did not affect MMP1 and MMP3 expression in normal human dermal fibroblasts and normal human keratinocytes. 84 AgNPs 68.9 nm in size may increase mRNA levels of MMP3, MMP11 and MMP19 in RAW264.7 cells.…”

Section: Discussionmentioning

confidence: 99%

Silver Nanoparticles as Chlorhexidine and Metronidazole Drug Delivery Platforms: Their Potential Use in Treating Periodontitis

Steckiewicz¹,

Cieciórski²,

Barcińska³

et al. 2022

IJN

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Purpose Periodontal disease (PD), defined as oral inflammation caused by dental plaque, is an emerging problem. PD may lead to tooth loss, and treatment options are limited. In this study, we designed, synthesized, and characterized silver nanoparticles (AgNPs) conjugated with chlorhexidine (AgNPs-CHL) or metronidazole (AgNPs-PEG-MET) to determine whether they can be used to treat PDs. Materials and Methods AgNPs were synthesized and characterized by transmission electron microscopy, UV-vis spectrometry, thermogravimetric analyses, and dynamic light scattering. We determined the safety and the antimicrobial and anti-inflammatory properties of synthesized AgNPs in an in vitro model of periodontitis. Antimicrobial properties were determined by measuring the minimum inhibitory concentration (MIC) and minimum biofilm eradication concentration (MBEC) on reference strains of bacteria and fungi. Human gingival fibroblast (HGF-1), murine macrophage (RAW264.7) and human foetal osteoblast (hFOB1.19) cells were used in the study. Lipopolysaccharide (LPS) was used to induce inflammation. Cytokine levels were measured using an enzyme-linked immunosorbent assay; metalloproteinase expression was measured using Western blotting. Results The synthesized AgNPs were spherical and narrow-dispersed with an average diameter of 13.4 nm ± 3.0 nm in the case of AgNPs-CHL and 3.72 nm ± 0.72 nm in the case of AgNPs-PEG-MET. Both types of AgNPs were active against bacteria and fungi. AgNPs-CHL proved to be a more potent antimicrobial agent, although they were more cytotoxic than AgNPs-PEG-MET; however, both demonstrated beneficial properties in nontoxic concentrations. AgNPs-CHL and AgNPs-PEG-MET decreased the production of proinflammatory cytokines IL-1β, IL-6, IL-8 and TNFα. Both agents also decreased the levels of metalloproteinases MMP3 and MMP8, which may indicate that they will inhibit tissue degradation. Conclusion AgNPs-CHL and AgNPs-PEG-MET may be possible therapeutic options for PD, as they have antibacterial and anti-inflammatory properties. However, to fully understand the potential of AgNPs, our in vitro findings must be evaluated in an in vivo model.

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Section: Discussionmentioning

confidence: 99%

Silver Nanoparticles as Chlorhexidine and Metronidazole Drug Delivery Platforms: Their Potential Use in Treating Periodontitis

Steckiewicz¹,

Cieciórski²,

Barcińska³

et al. 2022

IJN

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“…Treating fibroblasts with soy peptides results in a non-toxic outcome similar to that in the positive control, AA2G (vitamin C derivative) [34]. Increased proliferation occurs as a result of extracting low-molecular-weight, physiologically active peptides from soybean protein fermented with L. rhamnosus and processing them in fibroblasts at different concentrations [35]. Therefore, the main functional ingredient derived from soybeans exhibits low toxicity and increases the proliferation of human dermal fibroblast cells.…”

Section: Cytotoxicities Of Soymilk Fractions In Fibroblastsmentioning

confidence: 93%

“…Additionally, 100SMKFUF5, which showed the highest toxicity, had a cell viability of 59.63%, indicating that approximately 40% of the cell population was killed compared to that in the control. Kim et al [35] reported no cytotoxicity in HaCaT keratinocytes treated with various concentrations of soybean peptides fermented with L. rhamnosus, even at a high concentration of 0.2%. Overall, proliferation increased when samples were treated with fibroblast cells, but toxicity was higher when HaCaT cells were treated with the 100% soymilk fraction than…”

Section: Cytotoxicities Of Soymilk Fractions In Hacat Keratinocytesmentioning

confidence: 99%

Section: Cytotoxicities Of Soymilk Fractions In Hacat Keratinocytesmentioning

confidence: 99%

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The Radical Scavenging Activities and Anti-Wrinkle Effects of Soymilk Fractions Fermented with Lacticaseibacillus paracasei MK1 and Their Derived Peptides

Lee¹,

Choi²,

Jeong

et al. 2023

Antioxidants

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Soybean-derived peptides exert several beneficial effects in various experimental models. However, only a few studies have focused on the radical scavenging and anti-wrinkle effects of soymilk-derived peptides produced via different processes, such as fermentation, enzymatic treatment, and ultrafiltration. Therefore, in this study, we investigated the radical scavenging and antiwrinkle effects of soymilk fractions produced using these processes. We found that 50SFMKUF5, a 5 kDa ultrafiltration fraction fermented with Lacticaseibacillus paracasei MK1 after flavourzyme treatment, exhibited the highest radical scavenging activity using the 2,2-diphenyl-1-picrylhydrazyl radical scavenging assay as well as potent anti-wrinkle effects assessed by type 1 procollagen production and tumor necrosis factor-α production in ultraviolet B (UVB)-treated human dermal fibroblasts and HaCaT keratinocytes. To identify potential bioactive peptides, candidate peptides were synthesized, and their anti-wrinkle effects were assessed. APEFLKEAFGVN (APE), palmitoyl-APE, and QIVTVEGGLSVISPK peptides were synthesized and used to treat UVB-irradiated fibroblasts, HaCaT keratinocytes, and α-melanocyte-stimulating hormone-induced B16F1 melanoma cells. Among these peptides, Pal-APE exerted the strongest effect. Our results highlight the potential of soymilk peptides as anti-aging substances.

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The Inhibitory Effect of Metronidazole and Doxycycline-HCl on proMMP-3 Production in Gingival Fibroblast

Cited by 2 publications

References 2 publications

Silver Nanoparticles as Chlorhexidine and Metronidazole Drug Delivery Platforms: Their Potential Use in Treating Periodontitis

Silver Nanoparticles as Chlorhexidine and Metronidazole Drug Delivery Platforms: Their Potential Use in Treating Periodontitis

The Radical Scavenging Activities and Anti-Wrinkle Effects of Soymilk Fractions Fermented with Lacticaseibacillus paracasei MK1 and Their Derived Peptides

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