The inhibitory effect of different patterns of low frequency stimulation on neuronal firing following epileptiform activity in rat hippocampal slices

Ghasemi, Zahra; Naderi, Nima; Shojaei, Amir; Raoufy, Mohammad Reza; Ahmadirad, Nooshin; Barkley, Victoria

doi:10.1016/j.brainres.2018.11.012

Cited by 12 publications

(6 citation statements)

References 42 publications

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“…Previous studies have analyzed the effects of neuronal activation or inhibition after alternating low-frequency current stimulation in vivo , but the biophysics of these processes and their net effects in connected neuronal networks remain elusive (Sandkühler et al, 1997 ; Durand et al, 2006 ; Cavarretta et al, 2014 ; Ye and Steiger, 2015 ; Ghasemi et al, 2019 ). After 1 week of sinusoidal epidural stimulation of the sensory parietal cortex in rats, with currents at different frequencies (20, 40, 60, and 100 Hz), c-Fos immunoreactivity was highest at 40 Hz and decreased at 60 Hz and 100 Hz, also suggesting a frequency-dependent effect on excitatory and inhibitory neurons (Ryu et al, 2019 ).…”

Section: Discussionmentioning

confidence: 99%

Comparing the effects of transcranial alternating current and temporal interference (tTIS) electric stimulation through whole-brain mapping of c-Fos immunoreactivity

Carmona-Barrón

Campo²,

Delgado‐García³

et al. 2023

Front. Neuroanat.

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The analysis of the topography of brain neuromodulation following transcranial alternating current (AC) stimulation is relevant for defining strategies directed to specific nuclei stimulation in patients. Among the different procedures of AC stimulation, temporal interference (tTIS) is a novel method for non-invasive neuromodulation of specific deep brain targets. However, little information is currently available about its tissue effects and its activation topography in in vivo animal models. After a single session (30 min, 0.12 mA) of transcranial alternate current (2,000 Hz; ES/AC group) or tTIS (2,000/2,010 Hz; Es/tTIS group) stimulation, rat brains were explored by whole-brain mapping analysis of c-Fos immunostained serial sections. For this analysis, we used two mapping methods, namely density-to-color processed channels (independent component analysis (ICA) and graphical representation (MATLAB) of morphometrical and densitometrical values obtained by density threshold segmentation. In addition, to assess tissue effects, alternate serial sections were stained for glial fibrillary acidic protein (GFAP), ionized calcium-binding adapter molecule 1 (Iba1), and Nissl. AC stimulation induced a mild superficial increase in c-Fos immunoreactivity. However, tTIS stimulation globally decreased the number of c-Fos-positive neurons and increased blood brain barrier cell immunoreactivity. tTIS also had a stronger effect around the electrode placement area and preserved neuronal activation better in restricted areas of the deep brain (directional stimulation). The enhanced activation of intramural blood vessels’ cells and perivascular astrocytes suggests that low-frequency interference (10 Hz) may also have a trophic effect.

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Section: Discussionmentioning

confidence: 99%

Comparing the effects of transcranial alternating current and temporal interference (tTIS) electric stimulation through whole-brain mapping of c-Fos immunoreactivity

Carmona-Barrón

Campo²,

Delgado‐García³

et al. 2023

Front. Neuroanat.

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“…Improving effects in the lithium-pilocarpine model indicated following subiculum electrical stimulation with 1 Hz frequency, 0.1 ms pulse duration, and 300 μA intensity ( Zhong et al, 2012 ). Inhibition of epileptiform activity (high K + , Low Mg 2+ models) reported following Schaefer collateral electrical stimulation with a frequency lower than 5 Hz ( Ghasemi et al, 2018a ; Ghasemi et al, 2019 ; Ghasemi et al, 2018b ). Besides, a decrement in the occurrence and duration of seizures was demonstrated following LFS (1–3 Hz) delivery to the dorsal hippocampus in the genetic model of epilepsy and bilaterally to the thalamus in the pilocarpine model ( Kile et al, 2010 ).…”

Section: Deep Brain Stimulation (Dbs)mentioning

confidence: 99%

Neurostimulation as a Putative Method for the Treatment of Drug-resistant Epilepsy in Patient and Animal Models of Epilepsy

Bakhtiarzadeh

Zare

Ghasemi

et al. 2023

BCN

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A patient with epilepsy was shown to have neurobiological, psychological, cognitive, and social issues as a result of recurring seizures, which is regarded as a chronic brain disease. However, despite numerous drug treatments, approximately, 30%-40% of all patients are resistant to antiepileptic drugs. Therefore, newer therapeutic modalities are introduced into clinical practice which involve neurostimulation and direct stimulation of the brain. Hence, we review published literature on vagus nerve stimulation, trigeminal nerve stimulation, applying responsive stimulation systems, and deep brain stimulation (DBS) in animals and epileptic patient with an emphasis on drug-resistant epilepsy.

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“…24,25 Regarding time of application, our previous experiment showed that DBS had a better anticonvulsant action when applied at seizure onset, that is, when epileptiform activity begins, compared to DBS applied before and/or after epileptiform activity. 26 Considering the hippocampus' role in seizure development and the hippocampus' functional connectivity with the mPFC in memory and learning, in the present study, the effects of low-frequency DBS on seizure severity, the functional connectivity between the hippocampus and mPFC, as well as learning and memory were investigated.…”

Section: Introductionmentioning

confidence: 99%

“…Regarding time of application, our previous experiment showed that DBS had a better anticonvulsant action when applied at seizure onset, that is, when epileptiform activity begins, compared to DBS applied before and/or after epileptiform activity. 26 …”

Section: Introductionmentioning

confidence: 99%

Effect of the closed‐loop hippocampal low‐frequency stimulation on seizure severity, learning, and memory in pilocarpine epilepsy rat model

Zare,

Rezaei,

Nazari

et al. 2024

CNS Neurosci Ther

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AimsIn this study, the anticonvulsant action of closed‐loop, low‐frequency deep brain stimulation (DBS) was investigated. In addition, the changes in brain rhythms and functional connectivity of the hippocampus and prefrontal cortex were evaluated.MethodsEpilepsy was induced by pilocarpine in male Wistar rats. After the chronic phase, a tripolar electrode was implanted in the right ventral hippocampus and a monopolar electrode in medial prefrontal cortex (mPFC). Subjects' spontaneous seizure behaviors were observed in continuous video recording, while the local field potentials (LFPs) were recorded simultaneously. In addition, spatial memory was evaluated by the Barnes maze test.ResultsApplying hippocampal DBS, immediately after seizure detection in epileptic animals, reduced their seizure severity and duration, and improved their performance in Barnes maze test. DBS reduced the increment in power of delta, theta, and gamma waves in pre‐ictal, ictal, and post‐ictal periods. Meanwhile, DBS increased the post‐ictal‐to‐pre‐ictal ratio of theta band. DBS decreased delta and increased theta coherences, and also increased the post‐ictal‐to‐pre‐ictal ratio of coherence. In addition, DBS increased the hippocampal‐mPFC coupling in pre‐ictal period and decreased the coupling in the ictal and post‐ictal periods.ConclusionApplying closed‐loop, low‐frequency DBS at seizure onset reduced seizure severity and improved memory. In addition, the changes in power, coherence, and coupling of the LFP oscillations in the hippocampus and mPFC demonstrate low‐frequency DBS efficacy as an antiepileptic treatment, returning LFPs to a seemingly non‐seizure state in subjects that received DBS.

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The inhibitory effect of different patterns of low frequency stimulation on neuronal firing following epileptiform activity in rat hippocampal slices

Cited by 12 publications

References 42 publications

Comparing the effects of transcranial alternating current and temporal interference (tTIS) electric stimulation through whole-brain mapping of c-Fos immunoreactivity

Comparing the effects of transcranial alternating current and temporal interference (tTIS) electric stimulation through whole-brain mapping of c-Fos immunoreactivity

Neurostimulation as a Putative Method for the Treatment of Drug-resistant Epilepsy in Patient and Animal Models of Epilepsy

Effect of the closed‐loop hippocampal low‐frequency stimulation on seizure severity, learning, and memory in pilocarpine epilepsy rat model

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