The inhibitory effect and possible mechanisms of D-allose on cancer cell proliferation

Sui, Li; Dong, Yan; Watanabe, Yasuo; Yamaguchi, Fuminori; Hatano, Naoya; Tsukamoto, Ikuko; Izumori, Ken; Tokuda, Masaaki

doi:10.3892/ijo.27.4.907

Cited by 44 publications

(50 citation statements)

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“…We have previously shown that the rare sugar d-allose strongly induces TXNIP and inhibits cancer cell growth (Sui et al 2005a). Both d-allose and d-glucose are monosaccharides, and d-allose is a C-3 epimer of d-glucose with 80% of the sweetness of sucrose.…”

Section: Introductionmentioning

confidence: 99%

D-Allose Inhibits Cancer Cell Growth by Reducing GLUT1 Expression

Noguchi

Kamitori

Hossain

et al. 2016

Tohoku J. Exp. Med.

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Glucose is a major energy source for mammalian cells and is transported into cells via cell-specific expression of various glucose transporters (GLUTs). Especially, cancer cells require massive amounts of glucose as an energy source for their dysregulated growth and thus over-express GLUTs. d-allose, a C-3 epimer of d-glucose, is one of rare sugars that exist in small quantities in nature. We have shown that d-allose induces the tumor suppressor gene coding for thioredoxin interacting protein (TXNIP) and inhibits cancer cell growth by G1 cell cycle arrest. It has also been reported that GLUTs including GLUT1 are overexpressed in many cancer cell lines, which may contribute to larger glucose utilization. Since d-allose suppresses the growth of cancer cells through the upregulation of TXNIP expression, our present study focused on whether d-allose down-regulates GLUT1 expression via TXNIP expression and thus suppresses cancer cell growth. Western blot and real-time PCR analyses revealed that d-allose significantly induced TXNIP expression and inhibited GLUT1 expression in a dose-dependent manner in three human cancer cell lines: hepatocellular carcinoma (HuH-7), Caucasian breast adenocarcinoma (MDA-MB-231), and neuroblastoma (SH-SY5Y). In these cell lines, d-allose treatment inhibited cell growth. Importantly, d-allose treatment decreased glucose uptake, as measured by the uptake of 2-deoxy d-glucose. Moreover, the reporter assays showed that d-allose decreased the expression of luciferase through the hypoxia response element present in the tested promoter region. These results suggest that d-allose may cause the inhibition of cancer growth by reducing both GLUT1 expression and glucose uptake.

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Section: Introductionmentioning

confidence: 99%

D-Allose Inhibits Cancer Cell Growth by Reducing GLUT1 Expression

Noguchi

Kamitori

Hossain

et al. 2016

Tohoku J. Exp. Med.

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“…18,21 Based on that fact, the p53 accumulation by anti-cancer drugs was determined to confirm the signal retention to the ΔΨm change for the signaling echo method, because Hep G2 has a wild-type p53 protein. 18 As portrayed in Fig.…”

Section: Determination Of Signal Retentionmentioning

confidence: 99%

“…In contrast, d-allose is a natural monosaccharide of rare sugar used as a chemopreventive food because of the development of industrial production methods for it. [21][22][23] Furthermore, this type of compound can be a new class of chemotherapeutic drug. Actually, d-allose induces apoptosis through G2/M cell cycle arrest, and up-regulation of CdK inhibitors of p21 (binding to CdK1 and CdK2) and p27 (binding to cyclin E-CdK2).…”

Section: Introductionmentioning

confidence: 99%

“…These two anti-cancer drugs induce apoptosis via different mechanisms, but they each reportedly up-regulate the p53 protein level. 20,22 The principal tumor-suppressor p53 protein plays a key role in cell cycle arrest and apoptosis in cancer cells. 12,17,18,24,25 In fact, p53 can both up-regulate genes, such as PUMA, Noxa, and p53AIP1, and down-regulate Bax to trigger transcriptional apoptosis; it can also directly translocate to the mitochondria preceding ΔΨm decrease and caspase activation to induce non-transcriptional apoptosis.…”

Section: Introductionmentioning

confidence: 99%

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Development of Signaling Echo Method for Cell-based Quantitative Efficacy Evaluation of Anti-cancer Drugs in Apoptosis without Drug Presence Using High-precision Surface Plasmon Resonance Sensing

et al. 2010

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“…D-Allose has been reported to have no effect on normal human cell lines, 2,9) but inhibited the proliferation of human ovarian, 1) prostate, 2) liver, 3) leukemia, 4) and tongue 10) cancer cell lines. D-Allose may therefore be a potential anti-cancer compound with few side effects.…”

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confidence: 99%