“…The latter already slightly harms the original lactose containing selective media. It renders the glucose variants almost useless, because the tendency to produce inhibitory Maillard-complexes (Ingram et al, 1955;Jemmali, 1969;Einarsson et al, 1983;Vadehra and Bellamy, 1983) is very much stronger with glucose than with lactose. There is, incidentally, also no need to sterilize media for Enterobacteriaceae; the only organisms that might survive heating for about 30 rain at 80-100°C are spore-bearing bacilli and clostfidia which will be unable to develop in media containing bile salts as well as a triphenylmethane dye (Mossel et al, 1974).…”