The inhibition of sulphate incorporation in isolated adult chondrocytes by hyaluronic acid

Wiebkin, Ole W.; Muir, Helen

doi:10.1016/0014-5793(73)80422-3

Cited by 114 publications

(31 citation statements)

References 10 publications

(6 reference statements)

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“…These were 24-h experiments, and the long-term influence of matrix displacement via HAs on the chondrocyte phenotype is currently under study. Additionally, through the interaction of these matrix receptors with extracellular components, chondrocytes may fine tune their patterns of matrix biosynthesis and/or turnover, as has been previously suggested by several investigators (Wiebkin and Muir, 1973;Solursh et al, 1974Solursh et al, , 1980Handley and Lowther, 1976;Bansal et al, 1986;Morales and Hascail, 1988).…”

Section: Discussionmentioning

confidence: 79%

Hyaluronan receptor-directed assembly of chondrocyte pericellular matrix.

Knudson

1993

The Journal of Cell Biology

269

250

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Abstract. Initial assembly of extracellular matrix occurs within a zone immediately adjacent to the chondrocyte cell surface termed the cell-associated or periceUular matrix. Assembly within the periceUular matrix compartment requires specific cell-matrix interactions to occur, that are mediated via membrane receptors. The focus of this study is to elucidate the mechanisms of assembly and retention of the cartilage pericellular matrix proteoglycan aggregates important for matrix organization. Assembly of newly synthesized chondrocyte pericellular matrices was inhibited by the addition to hyaluronan hexasaccharides, competitive inhibitors of the binding of hyaluronan to its cell surface receptor. Fully assembled chondrocyte pericellular matrices were displaced using hyaluronan hexasaccharides as well.When exogenous hyaluronan was added to matrixfree chondrocytes in combination with aggrecan, a pericellular matrix equivalent in size to an endogenous matrix formed within 30 min of incubation. Addition of hyaluronan and aggrecan to glutaraldehyde-fixed chondrocytes resulted in matrix assembly comparable to live chondrocytes. These matrices could be inhibited from assembling by the addition of excess hyaluronan hexasaccharides or displaced once assembled by subsequent incubation with hyaluronan hexasaccharides. The results indicate that the aggrecanrich chondrocyte pericellular matrix is not only on a scaffolding of hyaluronan, but actually anchored to the cell surface via the interaction between hyaluronan and hyaluronan receptors.

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Section: Discussionmentioning

confidence: 79%

Hyaluronan receptor-directed assembly of chondrocyte pericellular matrix.

Knudson

1993

The Journal of Cell Biology

269

250

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“…Again, HA6.8 deviated the most and showed extreme PG synthesis suppression. PG synthesis suppression by low mass HA forms has been reported by others [47]. However, nearly all of the HA forms that enhanced repair also stimulated PG synthesis in cartilage explants.…”

Section: Day 7 Day 14mentioning

confidence: 84%

The role of insulin-like growth factor-I in hyaluronan mediated repair of cultured cartilage explants

2004

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“…Hyaluronate binds to the surface of various cell types (14) and may, under some circumstances, inhibit differentiation (9,(24)(25)(26)(27)(28). In tumor cells, this binding may also influence interactions with cells of the immune system, providing protection from cytolysis.…”

Section: Resultsmentioning

confidence: 99%

Hyaluronate and invasiveness of the rabbit V2 carcinoma.

Toole¹,

Biswas²,

Gross³

1979

Proc. Natl. Acad. Sci. U.S.A.

208

110

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We propose that hyaluronate, a major component of extracellular matrices through which cells migrate during embryonic tissue development and in regenerative processes, is also concentrated in the environment through which neoplastic cells invade local host tissues and may facilitate this process. The hyaluronate content of invasive V2 carcinoma grown in rabbit was found to be 34 times greater than that of the same tumor grown in the nude mouse, in which it is noninvasive. Moreover, hyaluronate concentrations were most elevated in the connective tissue interface between the tumor mass and the neighboring host tissue in the invasive rabbit tumors. The particular site of tumor implantation in the rabbit or nude mouse did not affect the concentrations of hyaluronate in either the parenchyma or the surrounding connective tissue. Similar values were obtained for neoplasms grown in muscle, which normally contains little hyaluronate, and in subcutaneous tissue, which is relatively rich in this glycosaminoglycan. The extracellular environment through which cells migrate during embryonic development and repair processes is enriched in the glycosaminoglycan (GAG) hyaluronate. Examples are found in the developing embryonic cornea (1), sclerotome (2), neural crest (3-5), heart (6, 7), and primary mesenchyme (8), as well as in regenerating salamander limb (9), regenerating rabbit tendon (10), and bone fracture callus (11). In certain of these tissues-e.g., the embryonic cornea (1) and neural crest (3-5, 12)-a close correlation between high concentrations of hyaluronate, high levels of hydration, and increased matrix volume with cell migration has been observed. On the basis of these studies we have proposed (i) that, due to its unique hydrodynamic properties (13), hyaluronate exerts a swelling pressure that can cause alterations in the size of intercellular spaces and separation of cell and connective tissue layers, thus opening avenues for cell migration, and (ii) that hyaluronate binds to the surface of cells (14), influencing specific cell interactions that might regulate the timing of cell assembly and differentiation (for review, see ref. 15).We are now examining the proposition that tumor cell migration into neighboring host tissue (i.e., invasion) also requires a hyaluronate-enriched environment. Previous studies in this laboratory of the cellular source of collagenase in the V2 carcinoma compared this neoplasm in the rabbit with that in the nude mouse (C. Biswas, K. J. Bloch, and J. Gross, unpublished results). Histological examination showed infiltration of tumor cells into and beyond the surrounding connective tissue (here called "capsule") of the rabbit tumors but not that of the nude mouse tumors. The capsules of the latter appeared to be intact around the entire neoplasm whereas, in the rabbit, sections of the tumor-host interface were fused, giving rise to discontinuities in the capsule and infiltration of tumor cells. The rabbit intramuscular and subcutaneous tumors have also been shown to be reproduc...

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The inhibition of sulphate incorporation in isolated adult chondrocytes by hyaluronic acid

Cited by 114 publications

References 10 publications

Hyaluronan receptor-directed assembly of chondrocyte pericellular matrix.

Hyaluronan receptor-directed assembly of chondrocyte pericellular matrix.

The role of insulin-like growth factor-I in hyaluronan mediated repair of cultured cartilage explants

Hyaluronate and invasiveness of the rabbit V2 carcinoma.

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