The inhibition of inflammatory molecule expression on 3T3-L1 adipocytes by berberine is not mediated by leptin signaling

Abstract

In our previous study, we have shown that berberine has both anti-adipogenic and anti-inflammatory effects on 3T3-L1 adipocytes, and the anti-adipogenic effect is due to the down-regulation of adipogenic enzymes and transcription factors. Here we focused more on anti-inflammatory effect of berberine using real time RT-PCR and found it changes expressions of adipokines. We hypothesized that anti-adipogenicity of berberine mediates anti-inflammtory effect and explored leptin as a candidate mediator of this signaling. We studied this hypothesis by western blot analysis, but our results showed that berberine has no effect on the phosphorylations of STAT-3 and ERK which have important roles on leptin signaling. These results led us to conclude that the anti-inflammatory effect of berberine is not mediated by the inhibition of leptin signal transduction. Moreover, we have found that berberine down-regulates NF-κB signaling, one of the inflammation-related signaling pathway, through western blot analysis. Taken together, the anti-inflammatory effect of berberine is not mediated by leptin, and berberine induces anti-inflammatory effect independent of leptin signaling.

Berberine suppresses neuroinflammatory responses through AMP‐activated protein kinase activation in BV‐2 microglia

“…Recently, it have shown that berberine has no effect on leptininduced STAT-3 phosphorylation [Choi et al, 2009]. Here we found that berberine also antagonizes LPS-induced IkBa activation and IFN-g-induced STAT-1 and STAT-3 phosphorylation (Fig.…”

“…Therefore, the inhibition of MAP kinase involved in berberine treatment might be dependent on cell types and experimental condition. Notably, numerous reports have also shown that berberine suppresses IL-1b and TNF-a production via the inhibition of NF-kB signaling pathway [Hsiang et al, 2005;Lee et al, 2007; Ho et al, 2009].Recently, it have shown that berberine has no effect on leptininduced STAT-3 phosphorylation [Choi et al, 2009]. Here we found that berberine also antagonizes LPS-induced IkBa activation and IFN-g-induced STAT-1 and STAT-3 phosphorylation (Fig.…”

Biological activity of Bauhinia racemosa against Diabetes and Interlinked Disorders like Obesity and Hyperlipidemia

“…BR extract showed more than 75% of viability of cells during in-vitro study. The extract of BR revealed significant inhibition in differentiated cell as compared to non-differentiated cell in 3T3-L1 cell line [31]. Improvement in lipid profile reduces the possibilities of diabetes induced obesity and cardiovascular diseases [32].…”

The Role of Adipocyte Mediators, Inflammatory Markers and Vitamin D in Gestational Diabetes

“…The association between fasting glucose and log-transformed 25OHD concentration was of borderline significance after accounting for ethnicity, age and body mass index in multivariate analyses. The odds ratio of gestational diabetes in women with 25OHD < 50 nmol/L did not reach statistical significance (1.92, 95% confidence interval 0.89-4.17) (Clifton-Bligh et al, 2008). In another study total prevalence of vitamin D deficiency (<25 nmol/L) was 70.6% in pregnant women.…”

“…Supplementation by varying forms of adiponectin improved control of insulin, blood glucose and triglyceride levels in mouse models. Berbelin, a herbal folk medicine, has been shown to increase adiponectin expression which partly explains its advantageous effects on metabolic disturbances (Choi et al, 2009). A reduction in adiponectin expression is associated with insulin resistance in animal models.…”