The inhibition of <i>Pseudomonas aeruginosa</i> biofilm formation by micafungin and the enhancement of antimicrobial agent effectiveness in BALB/c mice

Kissoyan, Kohar Annie B.; Bazzi, Wael; Hadi, Usamah; Matar, Ghassan M.

doi:10.1080/08927014.2016.1199021

Cited by 11 publications

(13 citation statements)

References 22 publications

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“…However, only CAS, at 64 μg/ml, was able to inhibit P. aeruginosa 151 biofilm ( Table 2). Previous studies have shown that MFG requires high concentrations (10 mg/ml) to have antibiofilm effect against P. aeruginosa isolates (Bazzi et al, 2013;Kissoyan et al, 2016) corroborating with our results which even higher concentration tested we did not observe any biofilm inhibition ( Table 2).…”

Section: Resultssupporting

confidence: 93%

“…and bacterial pathogens. In fact, while antibacterial activity of echinocandins has been restricted to the evaluation of MFG against P. aeruginosa and S. aureus (Bazzi et al, 2013;Kissoyan et al, 2016;Lown et al, 2016;Rasheed et al, 2018), CAS has only been evaluated against S. aureus, so far (Siala et al, 2016;Moore et al, 2020). Interestingly, in this study, we report for the first time that CAS alone was able to inhibit and reduce the viability of P. aeruginosa, and this antibacterial activity was extended to critical priority MDR and carbapenemase-producing P. aeruginosa lineages belonging to the international sequence type ST277, which is endemic in Brazilian hospitals (Nascimento et al, 2016).…”

Section: Resultsmentioning

confidence: 99%

“…In addition, echinocandins are capable of non-competitively inhibiting the glycosyltransferase encoded by the ndvB gene, which acts in the production of the bacterial β-(1,3)-D-glucan (Beaudoin et al, 2012) presents in P. aeruginosa cell wall and EPM from biofilms contributing to mixed biofilm susceptibility (Bazzi et al, 2013). In this regard, combined treatment of MFG with ceftazidime, levofloxacin, ciprofloxacin, or aztreonam increases the survival rates of mice infected with P. aeruginosa (Kissoyan et al, 2016). In addition, CAS combined with fluoroquinolones acts as facilitator agent for penetration of the antibacterials into the S. aureus biofilms (Siala et al, 2016), as well as anidulafungin, another echinocandin agent, acts synergistically when combined with tigecycline against in vivo intra-abdominal mixed biofilms formed by C. albicans and S. aureus (Rogiers et al, 2018).…”

Section: Resultsmentioning

confidence: 99%

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Caspofungin and Polymyxin B Reduce the Cell Viability and Total Biomass of Mixed Biofilms of Carbapenem-Resistant Pseudomonas aeruginosa and Candida spp.

et al. 2020

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Pseudomonas aeruginosa and Candida spp. are biofilm-forming pathogens commonly found colonizing medical devices, being mainly associated with pneumonia and bloodstream infections. The coinfection by these pathogens presents higher mortality rates when compared to those caused by a single microbial species. This study aimed to evaluate the antibiofilm activity of echinocandins and polymyxin B (PMB) against polymicrobial biofilms of carbapenem-resistant (CR) Pseudomonas aeruginosa and Candida spp. (C. albicans, C. parapsilosis, C. tropicalis, and C. glabrata). In addition, we tested the antimicrobial effect on their planktonic and monomicrobial biofilm counterparties. Interestingly, beyond inhibition of planktonic [minimum inhibitory concentration (MIC) = 0.5 μg/ml] and biofilm [minimum biofilm inhibitory concentration (MBIC)50 ≤ 2–8 μg/ml] growth of P. aeruginosa, PMB was also effective against planktonic cells of C. tropicalis (MIC = 2 μg/ml), and polymicrobial biofilms of CR P. aeruginosa with C. tropicalis (MBIC50 ≤ 2 μg/ml), C. parapsilosis (MBIC50 = 4–16 μg/ml), C. glabrata (MBIC50 = 8–16 μg/ml), or C. albicans (MBIC50 = 8–64 μg/ml). On the other hand, while micafungin (MFG) showed highest inhibitory activity against planktonic (MIC ≤ 0.008–0.5 μg/ml) and biofilm (MBIC50 ≤ 2–16 μg/ml) growth of Candida spp.; caspofungin (CAS) displays inhibitory activity against planktonic cells (MIC = 0.03–0.25 μg/ml) and monomicrobial biofilms (MBIC50 ≤ 2–64 μg/ml) of Candida spp., and notably on planktonic and monomicrobial biofilms of CR P. aeruginosa (MIC or MBIC50 ≥ 64 μg/ml). Particularly, for mixed biofilms, while CAS reduced significantly viable cell counts of CR P. aeruginosa and Candida spp. at ≥32 and ≥ 2 μg/ml, respectively; PMB was effective in reducing viable cells of CR P. aeruginosa at ≥2 μg/ml and Candida spp. at ≥8 μg/ml. Similar reduction of viable cells was observed for CAS (32–64 μg/ml) combined with PMB (2 μg/ml). These findings highlight the potential of PMB and CAS for the treatment of polymicrobial infections caused by Candida spp. and critical priority CR P. aeruginosa.

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Section: Resultssupporting

confidence: 93%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Caspofungin and Polymyxin B Reduce the Cell Viability and Total Biomass of Mixed Biofilms of Carbapenem-Resistant Pseudomonas aeruginosa and Candida spp.

et al. 2020

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“…These include, skin, urinary tract, blood, wound, ear, and eye infections [2]. P. aeruginosa is characterized by its high ability to produce biofilms, making it a hard pathogen to treat and a powerful model for biofilm studies [3,4]. The biofilm's hallmark is enabling the bacteria within to adhere on living and nonliving surfaces as well as rendering the bacteria resistant to antibiotics and immune defense [5,6].…”

Section: Introductionmentioning

confidence: 99%

Natural products and polysorbates: Potential Inhibitors of biofilm formation in Pseudomonas aeruginosa

Miari

Rasheed

Haidar-Ahmad

et al. 2020

J Infect Dev Ctries

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Introduction: With all the challenges super bugs are imposing, biofilm formation opens the door against various more complicated challenges. Such issue may be highlighted with the ability of the latter to render the antibiotics hardly accessible to bacterial cells and sheds the light on the importance of finding antibiofilm formers. Therefore, we assessed the inhibitory effect of natural product extracts (ginger, wild blueberry) and polysorbates (PS20, PS80) on biofilm formation at the molecular level. Methodology: Growth inhibition assay was performed to test the effect of ginger (Zingiber Officinale), wild blueberry (Vaccinium Angustifolium), and polysorbates on Pseudomonas aeruginosa (PAN14) growth. Transcription levels of biofilm exopolysaccharides encoding genes (ndvB, pelC, algC) and quorum sensing genes (lasI, lasR, rhlI, rhlR) for LasI/LasR and RhlI/ RhlR systems were evaluated by RT qPCR. Results: The polysorbates and the extracts of both ginger and wild blueberry had no effect on the growth of P. aeruginosa. Biofilms’ examination has unraveled the effectiveness of treatments used in reducing its formation. Moreover, a significant reduction in the expression of all genes tested for biofilm exopolysaccharides and its quorum sensing system was observed. Conclusion: The decrease in the relative gene expression of the exopolysaccharides and quorum sensing encoding genes sheds the light on the mechanism of action of ginger and wild blueberry’s constituents as well as polysorbates 20 and 80 on P. aeruginosa biofilm formation. Future studies need to assess the antibiofilm effect of each fraction of herbal extracts separately.

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“…The findings are relevant in establishing the curative effects of micafungin on other alphavirus infections [195]. In association with levofloxacin, micafungin provides for a better outcome against P. aeruginosa induced infections under both in vitro and in vivo conditions [196].…”

Section: Antifungal Drugsmentioning

confidence: 99%

Potent antifungal agents and use of nanocarriers to improve delivery to the infected site: A systematic review

2021

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There are four major classes of antifungals with the predominant mechanism of action being targeting of cell wall or cell membrane. As in other drugs, low solubility of these compounds has led to low bioavailability in target tissues. Enhanced drug dosages have effects such as toxicity, drug–drug interactions, and increased drug resistance by fungi. This article reviews the current state‐of‐the‐art of antifungals, structure, mechanism of action, other usages, and toxic side effects. The emergence of nanoformulations to transport and uniformly release cargo at the target site is a boon in antifungal treatment. The article details research that lead to the development of nanoformulations of antifungals and potential advantages and avoidance of the lacunae characterizing conventional drugs. A range of nanoformulations based on liposomes, polymers are in various stages of research and their potential advantages have been brought out. It could be observed that under similar dosages, test models, and duration, nanoformulations provided enhanced activity, reduced toxicity, higher uptake and higher immunostimulatory effects. In most instances, the mechanism of antifungal activity of nanoformulations was similar to that of regular antifungal. There are possibilities of coupling multiple antifungals on the same nano‐platform. Increased activity coupled with multiple mechanisms of action presents for nanoformulations a tremendous opportunity to overcome antifungal resistance. In the years to come, robust methods for the preparation of nanoformulations taking into account the repeatability and reproducibility in action, furthering the studies on nanoformulation toxicity and studies of human models are required before extensive use of nanoformulations as a prescribed drug.

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The inhibition of Pseudomonas aeruginosa biofilm formation by micafungin and the enhancement of antimicrobial agent effectiveness in BALB/c mice

Cited by 11 publications

References 22 publications

Caspofungin and Polymyxin B Reduce the Cell Viability and Total Biomass of Mixed Biofilms of Carbapenem-Resistant Pseudomonas aeruginosa and Candida spp.

Caspofungin and Polymyxin B Reduce the Cell Viability and Total Biomass of Mixed Biofilms of Carbapenem-Resistant Pseudomonas aeruginosa and Candida spp.

Natural products and polysorbates: Potential Inhibitors of biofilm formation in Pseudomonas aeruginosa

Potent antifungal agents and use of nanocarriers to improve delivery to the infected site: A systematic review

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