The inhibition of glycosaminoglycan incorporation influences the cell proliferation and cytodifferentiation in cultured embryonic mouse molars

Jiang, Beizhan; Xu, Fangfang; Li, Lefeng; Chen, Weiting; Hong, Shebin; Chen, Rongmei

doi:10.1007/s10735-018-9803-2

Cited by 11 publications

(3 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Chondroitin sulfate (CS)/dermatan sulfate (DS), a class of natural polyanionic, linear and heterogeneous glycosaminoglycan (GAG), is ubiquitously distributed on the cell surface and the extracellular matrix (ECM) in animal tissues ( Cohen and Merzendorfer, 2019 ). CS/DS chains link with core proteins to form proteoglycans, interact with a series of proteins, such as chemokines ( Dyer et al, 2016 ; Sepuru and Rajarathnam, 2019 ), growth factors ( Sirko et al, 2010 ; Zhang et al, 2019 ) or other ECM components, and participate in various physiological and pathological processes ( Monneau et al, 2016 ; Jiang et al, 2019 ; Tighe and Garantziotis, 2019 ). The backbone of CS is composed of D-glucoronic acid (GlcUA) and N -acetyl-D-galactosamine (GalNAc) as the form of repeating disaccharide units GlcUAβ1–3GalNAc linked by β1–4 glycosidic bonds.…”

Section: Introductionmentioning

confidence: 99%

Identification and Action Patterns of Two Chondroitin Sulfate Sulfatases From a Marine Bacterium Photobacterium sp. QA16

Lin

Zhang

et al. 2022

Front. Microbiol.

View full text Add to dashboard Cite

Chondroitin sulfate (CS)/dermatan sulfate (DS) is a kind of sulfated polyanionic, linear polysaccharide belonging to glycosaminoglycan. CS/DS sulfatases, which specifically hydrolyze sulfate groups from CS/DS oligo-/polysaccharides, are potential tools for structural and functional studies of CD/DS. However, only a few sulfatases have been reported and characterized in detail to date. In this study, two CS/DS sulfatases, PB_3262 and PB_3285, were identified from the marine bacterium Photobacterium sp. QA16 and their action patterns were studied in detail. PB_3262 was characterized as a novel 4-O-endosulfatase that can effectively and specifically hydrolyze the 4-O-sulfate group of disaccharide GlcUAβ1–3GalNAc(4-O-sulfate) but not GlcUAβ1–3GalNAc(4,6-O-sulfate) and IdoUAα1–3GalNAc(4-O-sulfate) in CS/DS oligo-/polysaccharides, which is very different from the identified 4-O-endosulfatases in the substrate profile. In contrast, PB_3285 specifically hydrolyzes the 6-O-sulfate groups of GalNAc(6-O-sulfate) residues located at the reducing ends of the CS chains and is the first recombinantly expressed 6-O-exosulfatase to effectively act on CS oligosaccharides.

show abstract

Section: Introductionmentioning

confidence: 99%

Identification and Action Patterns of Two Chondroitin Sulfate Sulfatases From a Marine Bacterium Photobacterium sp. QA16

Lin

Zhang

et al. 2022

Front. Microbiol.

View full text Add to dashboard Cite

show abstract

“…Tooth germs were photographed at 0-day, 1 day, 3 day, 5 day and each tooth size (width, height and area) were measured (n=9 for each group) by using the modified method reported in the literature previously. Each experiment was repeated 3 times [43].…”

Section: Methodsmentioning

confidence: 99%

Overburdened Ferroptotic Stress Impairs Tooth Morphogenesis

Wang

et al. 2023

Preprint

View full text Add to dashboard Cite

Regulated cell death (RCD) has been reported to play a critical role in organ development. Despite apoptosis, the best known and most studied RCD in organogenesis, whether newly identified non-apoptotic cell death participates in the physiological process like development is barely understood. As a metabolic-related cell death pathway, ferroptosis, characterized by iron-dependent and lethal lipid peroxidation, was firstly described in 2012 and is now being wildly explored for its pathological function. Main factors of ferroptosis, lipid metabolism, ROS biology, and iron regulation, are also critically involved in developmental progress but hard to manipulate and investigate effectively in vivo through a high throughput way. Here in our study, an ex vivo culture model of tooth germ is applied to investigate the possible role of ferroptosis in organogenesis, since both RCD and risk factors of ferroptosis (lipid metabolism, ROS biology, and iron regulation) participate in tooth morphogenesis and the well-established ex vivo culture model enables effective manipulation of ferroptosis. To investigate whether ferroptotic stress exists and may influence tooth germ development, we detect the spatiotemporal expression of Gpx4, a core anti-lipid peroxidation enzyme in ferroptosis, in mouse development first molar and incisor. Morphological changes in each group with/without activation of ferroptosis are identified by gross anatomy, sequential HE staining, and related 3D reconstruction. Ferroptosis-related genes, lipid peroxidation, the morphology of mitochondria, and iron accumulation are detected to confirm the activation of ferroptosis. Inhibition of ferroptosis by DFO, Fer-1, and Lip-1, could partly rescue organogenesis of erastin-impaired tooth morphogenesis. Moreover, apoptosis is not significantly activated in erastin-impaired tooth germ indicated by cleavage of Caspase-3 and TUNEL staining. These results demonstrate that ferroptotic stress exists in tooth organogenesis but is well controlled by following upregulation of Gpx4; Overburdened ferroptotic stress cause by erastin significantly suppress tooth morphogenesis.

show abstract

“…GAGs are also indispensable in the later stages of differentiation such as cytodifferentiation. GAGs affect cell folding and formation of the inner enamel epithelium, as well as the subsequent odontoblast and ameloblast differentiation, as shown by the significantly reduced expression of AMELX, DMP-1, and DSPP in GAG-deficient tooth germs (Liu et al 2017; Jiang et al 2019). Notably, sulfation levels have been shown to be pivotal determinants of cytodifferentiation via the regulation of signaling transduction.…”

Section: Roles Of Glycosaminoglycans In Odontogenesismentioning

confidence: 99%

The Essential Role of Proteoglycans and Glycosaminoglycans in Odontogenesis

Chen,

Sun,

Lin

et al. 2024

J Dent Res

View full text Add to dashboard Cite

Tooth development and regeneration are regulated through a complex signaling network. Previous studies have focused on the exploration of intracellular signaling regulatory networks, but the regulatory roles of extracellular networks have only been revealed recently. Proteoglycans, which are essential components of the extracellular matrix (ECM) and pivotal signaling molecules, are extensively involved in the process of odontogenesis. Proteoglycans are composed of core proteins and covalently attached glycosaminoglycan chains (GAGs). The core proteins exhibit spatiotemporal expression patterns during odontogenesis and are pivotal for dental tissue formation and periodontium development. Knockout of core protein genes Biglycan, Decorin, Perlecan, and Fibromodulin has been shown to result in structural defects in enamel and dentin mineralization. They are also closely involved in the development and homeostasis of periodontium by regulating signaling transduction. As the functional component of proteoglycans, GAGs are negatively charged unbranched polysaccharides that consist of repeating disaccharides with various sulfation groups; they provide binding sites for cytokines and growth factors in regulating various cellular processes. In mice, GAG deficiency in dental epithelium leads to the reinitiation of tooth germ development and the formation of supernumerary incisors. Furthermore, GAGs are critical for the differentiation of dental stem cells. Inhibition of GAGs assembly hinders the differentiation of ameloblasts and odontoblasts. In summary, core proteins and GAGs are expressed distinctly and exert different functions at various stages of odontogenesis. Given their unique contributions in odontogenesis, this review summarizes the roles of proteoglycans and GAGs throughout the process of odontogenesis to provide a comprehensive understanding of tooth development.

show abstract

The inhibition of glycosaminoglycan incorporation influences the cell proliferation and cytodifferentiation in cultured embryonic mouse molars

Cited by 11 publications

References 41 publications

Identification and Action Patterns of Two Chondroitin Sulfate Sulfatases From a Marine Bacterium Photobacterium sp. QA16

Identification and Action Patterns of Two Chondroitin Sulfate Sulfatases From a Marine Bacterium Photobacterium sp. QA16

Overburdened Ferroptotic Stress Impairs Tooth Morphogenesis

The Essential Role of Proteoglycans and Glycosaminoglycans in Odontogenesis

Contact Info

Product

Resources

About