The inhibition effects of insulin on BMP2-induced muscle heterotopic ossification

Zhao, Yannan; Hou, Xun; Chen, Bing; Xiao, Zhifeng; Han, Jin Soo; Shi, Chunying; Liu, Jianzhou; Miao, Qi

doi:10.1016/j.biomaterials.2014.07.056

Cited by 13 publications

(7 citation statements)

References 49 publications

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“…Bone regeneration is regulated by various bioactive agents such as platelet derived growth factor (PDGF), transforming growth factor-beta (TGF- β ), bone morphogenetic proteins (BMPs), and insulin-like growth factor (IGF), which are important factors involved in the osteogenic process. Among the many BMPs, BMP2, a member of the highly conserved transforming growth factor- β superfamily, is a potent inducer of osteogenesis and plays an important role in bone formation and remodeling [ 12 – 15 ]. Although successful bone regeneration was induced by BMP2 in preclinical and clinical application, supraphysiological dosage of BMP2 was required, which may cause low efficiency and potentially toxic effect.…”

Section: Introductionmentioning

confidence: 99%

Fabrication of Core‐Shell PEI/pBMP2‐PLGA Electrospun Scaffold for Gene Delivery to Periodontal Ligament Stem Cells

Xie

Jia

Xu³

et al. 2016

Stem Cells International

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Bone tissue engineering is the most promising technology for enhancing bone regeneration. Scaffolds loaded with osteogenic factors improve the therapeutic effect. In this study, the bioactive PEI (polyethylenimine)/pBMP2- (bone morphogenetic protein-2 plasmid-) PLGA (poly(D, L-lactic-co-glycolic acid)) core-shell scaffolds were prepared using coaxial electrospinning for a controlled gene delivery to hPDLSCs (human periodontal ligament stem cells). The pBMP2 was encapsulated in the PEI phase as a core and PLGA was employed to control pBMP2 release as a shell. First, the scaffold characterization and mechanical properties were evaluated. Then the gene release behavior was analyzed. Our results showed that pBMP2 was released at high levels in the first few days, with a continuous release behavior in the next 28 days. At the same time, PEI/pBMP2 showed high transfection efficiency. Moreover, the core-shell electrospun scaffold showed BMP2 expression for a much longer time (more than 28 days) compared with the single axial electrospun scaffold, as evaluated by qRT-PCR and western blot after culturing with hPDLSCs. These results suggested that the core-shell PEI/pBMP2-PLGA scaffold fabricated by coaxial electrospinning had a good gene release behavior and showed a prolonged expression time with a high transfection efficiency.

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Section: Introductionmentioning

confidence: 99%

Fabrication of Core‐Shell PEI/pBMP2‐PLGA Electrospun Scaffold for Gene Delivery to Periodontal Ligament Stem Cells

Xie

Jia

Xu³

et al. 2016

Stem Cells International

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“…HO is an abnormal formation of mature, lamellar bone in soft tissues where bone normally does not exist. 40 HO is a well-known complication following surgical procedures or traumatic injuries. 41,42 Osteoinductive factors are released as a consequence of soft tissue trauma, thus potentially inducing formation of heterotopic bone.…”

Section: Discussionmentioning

confidence: 99%

Freeze-dried chitosan-platelet-rich plasma implants improve supraspinatus tendon attachment in a transosseous rotator cuff repair model in the rabbit

et al. 2018

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Rotator cuff tears result in shoulder pain, stiffness, weakness and loss of motion. After surgical repair, high failure rates have been reported based on objective imaging and it is recognized that current surgical treatments need improvement. The aim of the study was to assess whether implants composed of freeze-dried chitosan (CS) solubilized in autologous platelet-rich plasma (PRP) can improve rotator cuff repair in a rabbit model. Complete tears were created bilaterally in the supraspinatus tendon of New Zealand White rabbits (n ¼ 4 in a pilot feasibility study followed by n ¼ 13 in a larger efficacy study), which were repaired using transosseous suturing. On the treated side, CS-PRP implants were injected into the transosseous tunnels and the tendon itself, and healing was assessed histologically at time points ranging from one day to two months post-surgery. CS-PRP implants were resident within transosseous tunnels and adhered to tendon surfaces at one day post-surgery and induced recruitment of polymorphonuclear cells from 1 to 14 days. CS-PRP implants improved attachment of the supraspinatus tendon to the humeral head through increased bone remodelling at the greater tuberosity and also inhibited heterotopic ossification of the supraspinatus tendon at two months. In addition, the implants did not induce any detectable deleterious effects. This preliminary study provides the first evidence that CS-PRP implants could be effective in improving rotator cuff tendon attachment in a small animal model.

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“…In addition, the short biological half-life, weak stability, and demand for optimum controlled-release carriers for therapeutic applications limit their clinical use (6)(7)(8). There is evidence that BMPs stimulate osteoclastic activity and lack tissue-selectivity, which can lead to osteoclastic bone resorption (9,10) and ectopic bone formation (11). Therefore, simple, effective, safe and inexpensive bioactive factors need to be developed for treating bone diseases using bone regenerative medicine.…”

Section: Introductionmentioning

confidence: 99%

Icaritin, a novel plant-derived osteoinductive agent, enhances the osteogenic differentiation of human bone marrow- and human adipose tissue-derived mesenchymal stem cells

Shu

Kang

et al. 2017

International Journal of Molecular Medicine

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For the treatment of diseases affecting bones using bone regenerative medicine, there is an urgent need to develop safe, inexpensive drugs that can strongly induce bone formation. In the present study, we systematically investigated the effects of icaritin, a metabolic product of icariin, on the osteogenic differentiation of human bone marrow‑derived mesenchymal stem cells (hBMSCs) and human adipose tissue‑derived stem cells (hADSCs) in vitro. After treatment with icaritin at concentrations of 10‑8-10‑5 M, hBMSCs and hADSCs were examined for alkaline phosphatase activity, osteocalcin (OC) secretion, matrix mineralization and expression levels of bone‑related mRNA and proteins. Data showed that icaritin at concentrations 10‑7-10‑5 M significantly increased alkaline phosphatase activity, OC secretion at different time points, and calcium deposition at day 21. In addition, icaritin upregulated the mRNA expression of genes for bone morphogenetic proteins (BMP‑2, ‑4 and ‑7), bone transcription factors (Runx2 and Dlx5) and bone matrix proteins (ALP, OC and Col‑1). Moreover, icaritin increased the protein levels of BMPs, Runx2 and OC, as detected by western blot analysis. These findings suggest that icaritin enhances the osteogenic differentiation of hBMSCS and hADSCs. Icaritin exerts its potent osteogenic effect possibly by directly stimulating the production of BMPs. Although the osteogenic activity of icaritin in vitro was inferior to that of rhBMP‑2, icaritin displayed better results than icariin. Moreover, the low cost, simple extraction procedure, and an abundance of icaritin make it appealing as a bone regenerative medicine.

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The inhibition effects of insulin on BMP2-induced muscle heterotopic ossification

Cited by 13 publications

References 49 publications

Fabrication of Core‐Shell PEI/pBMP2‐PLGA Electrospun Scaffold for Gene Delivery to Periodontal Ligament Stem Cells

Fabrication of Core‐Shell PEI/pBMP2‐PLGA Electrospun Scaffold for Gene Delivery to Periodontal Ligament Stem Cells

Freeze-dried chitosan-platelet-rich plasma implants improve supraspinatus tendon attachment in a transosseous rotator cuff repair model in the rabbit

Icaritin, a novel plant-derived osteoinductive agent, enhances the osteogenic differentiation of human bone marrow- and human adipose tissue-derived mesenchymal stem cells

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