The inheritance of seed colour and vernalization requirement in Brassica napus using doubled haploid populations

Deynze, Allan Van; Pauls, K. Peter

doi:10.1007/bf00033770

Cited by 32 publications

(10 citation statements)

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“…Two duplicate and independent, dominant loci controlled pigment synthesis in B. juncea (Vera et al 1979;Vera and Woods 1982), while three incompletely dominant loci have been reported to control pigment synthesis in B. napus (Shirzadegan 1986;Van For personal use only.…”

Section: Discussionmentioning

confidence: 99%

Repression of seed coat pigmentation in Ethiopian mustard

Getinet¹,

Rakow²

1997

Can. J. Plant Sci.

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Getinet, A. and Rakow, G. 1997. Repression of seed coat pigmentation in Ethiopian mustard. Can. J. Plant Sci. 77: 501-505. The inheritance of seed colour in Brassica carinata A. Braun was investigated in backcross and F 2 generations derived from two crosses between the brown seeded cultivar S-67 and two, true breeding, yellow seeded lines, PGRC/E 21164 and PGRC/E 21224 of the Plant Gene Resources Centre of Ethiopia. F 1 seed was identical in colour to self-pollinated seed borne on the respective brown and yellow seeded parents indicating maternal control of seed colour in B. carinata. F 1 plants of reciprocal crosses produced yellow seed that was somewhat darker than that of the yellow-seeded parent indicating incomplete dominance of yellow over brown. Backcross F 1 plants, derived from the backcross to the brown parent, segregated brown and light yellow-brown/ yellow seeded plants in a 1:1 ratio, while backcrosses to both yellow seeded parents produced only light yellow-brown/yellow seeded plants. The F 2 generation segregated brown and light yellow-brown/yellow seeded plants in a 1:3 ratio. These results are in contrast to seed colour inheritance pattern observed in other Brassica species, where brown seed colour was fully or partially dominant over yellow. The apparent dominance of the absence of a gene product in yellow seed over its presence in brown seed, was explained by the presence of a dominant repressor gene (Rp) in yellow seed which inhibits the expression of seed coat pigment synthesis genes. The repressor gene is absent in brown seeded plants. The significance of this finding in relation to the development of yellow seeded lines in other Brassica species is discussed. Key words: Brassica carinata, seed colour inheritance, repressorGetinet, A. et Rakow, G. 1997. Inhibition de la pigmentation du tégument séminal chez la moutarde d'Éthiopie. Can. J. Plant Sci. 77: 501-505. Nous avons examiné le mode de transmission héréditaire de la coloration de la graine de Brassica carinata A. Braun dans des rétrocroisements et des générations F 2 issus de deux croisements entre le cultivar à graine brune S-67 et deux lignées homozygotes à graine jaune PGRC/E 21164 et PGRC/E 21224. Ces travaux étaient réalisés au Centre de ressources phytogénétiques de l'Éthiopie. Les graines F 1 avaient la même coloration que les graines obtenues par autopollinisation, portées par les parents respectifs à graines brunes et à graines jaunes, laissant penser à une régulation maternelle de la couleur des graines chez cette espèce. Le jaune des graines produites par les F 1 de croisements réciproques était quelque peu plus foncé que celui des graines du parent à graine jaune, ce qui suggérerait une dominance incomplète du jaune sur le brun. Les plantes F 1 dérivées du rétro-croisement au parent à graines brunes produisaient des descendances à graines brunes et à graines brun-jaune pâle/jaune dans la proportion 1:1, alors que dans le rétrocroisement aux deux parents à graine jaune les F 1 ne produisaient que des graines brun-jaune pâ...

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Section: Discussionmentioning

confidence: 99%

Repression of seed coat pigmentation in Ethiopian mustard

Getinet¹,

Rakow²

1997

Can. J. Plant Sci.

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“…The inheritance of seed coat color has been studied and several polygenic models have been proposed by different researchers to explain the complex segregation of seed coat color in different yellow-seeded varieties (Shirzadegan 1986;Liu 1992;Van Deynze and Pauls 1994;Rahman 2001;Liu et al 2005a). Some of the genes have been mapped using various molecular markers including RFLP (Van Deynze et al 1995), RAPD (Somers et al 2001), and AFLP (Liu et al 2005b).…”

Section: Discussionmentioning

confidence: 99%

“…Shi and K. Liu, unpublished data), indicating that the different genotypes have different seed coat color genes. Additionally, the influence of maternal genotype and environmental factors on seed coat color makes the inheritance of this trait more complex (Shirzadegan 1986;Van Deynze and Pauls 1994).…”

Section: Introductionmentioning

confidence: 99%

“…It has been shown that seed coat color in B. napus is controlled by 1 to as many as 4 genes. Therefore, several polygenic models (Shirzadegan 1986;Liu 1992;Van Deynze and Pauls 1994;Rahman 2001;Liu et al 2005a) have been proposed to explain seed coat color inheritance where yellow seeds are produced only when all loci were in the recessive homozygous condition. No.…”

Section: Introductionmentioning

confidence: 99%

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Generation and mapping of SCAR and CAPS markers linked to the seed coat color gene in Brassica napus using a genome-walking technique

Xiao

et al. 2007

Genome

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The yellow seed coat trait in No. 2127-17, a resynthesized purely yellow Brassica napus line, is controlled by a single partially dominant gene, Y. A double-haploid population derived from the F1 of No. 2127-17 x 'ZY821' was used to map the seed coat color phenotype. A combination of AFLP analysis and bulked segregant analysis identified 18 AFLP markers linked to the seed coat color trait. The 18 AFLP markers were mapped to a chromosomal region of 37.0 cM with an average of 2.0 cM between adjacent markers. Two markers, AFLP-K and AFLP-H, bracketed the Y locus in an interval of 1.0 cM, such that each was 0.5 cM away from the Y locus. Two other markers, AFLP-A and AFLP-B, co-segregated with the seed color gene. For ease of use in breeding programs, these 4 most tightly linked AFLP markers were converted into reliable PCR-based markers. SCAR-K, which was derived from AFLP-K, was assigned to linkage group 9 (N9) of a B. napus reference map consisting of 150 commonly used SSR (simple sequence repeat) markers. Furthermore, 2 SSR markers (Na14-E08 and Na10-B07) linked to SCAR-K on the reference map were reversely mapped to the linkage map constructed in this study, and also showed linkage to the Y locus. These linked markers would be useful for the transfer of the dominant allele Y from No. 2127-17 to elite cultivars using a marker-assisted selection strategy and would accelerate the cloning of the seed coat color gene.

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“…Attempts have been made to develop artificially synthesised yellow-seeded varieties of B. rapa, B. napus and B. juncea (Jönsson 1975;Abraham and Bhatia 1986;Rahman 2001) by using the yellow-seeded forms that exist in the natural germplasm of Brassica species. However, breeding for yellow-seeded varieties is complicated by multiple gene inheritance, allotetraploidy, maternal effect and environmental factors (Shizadigan 1986;Van Deynze and Pauls 1994). The identification of markers linked to seed coat colour will facilitate the breeding of yellow-seeded varieties in Brassica.…”

Section: Introductionmentioning

confidence: 99%

Mapping of AFLP markers linked to seed coat colour loci in Brassica juncea (L.) Czern

Sabharwal

Negi²,

Banga

et al. 2004

Theor Appl Genet

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Association mapping of the seed-coat colour with amplified fragment length polymorphism (AFLP) markers was carried out in 39 Brassica juncea lines. The lines had genetically diverse parentages and varied for seed-coat colour and other morphological characters. Eleven AFLP primer combinations were used to screen the 39 B. juncea lines, and a total of 335 polymorphic bands were detected. The bands were analysed for association with seed-coat colour using multiple regression analysis. This analysis revealed 15 markers associated with seed-coat colour, obtained with eight AFLP primer combinations. The marker E-ACA/M-CTG(350 )explained 69% of the variation in seed-coat colour. This marker along with markers E-AAC/M-CTC(235 )and E-AAC/M-CTA(250) explained 89% of the total variation. The 15 associated markers were validated for linkage with the seed-coat colour loci using a recombinant inbred line (RIL) mapping population. Bands were amplified with the eight AFLP primer combinations in 54 RIL progenies. Of the 15 associated markers, 11 mapped on two linkage groups. Eight markers were placed on linkage group 1 at a marker density of 6.0 cM, while the remaining three were mapped on linkage group 2 at a marker density of 3.6 cM. Marker E-ACA/M-CTG(350 )co-segregated with Gene1 controlling seed-coat colour; it was specific for yellow seed-coat colour and mapped to linkage group 1. Marker E-AAC/M-CTC(235) (AFLP8), which had been studied previously, was present on linkage group 2; it was specific for brown seed-coat colour. Since AFLP markers are not adapted for large-scale applications in plant breeding, it is important to convert these to sequence-characterised amplified region (SCAR) markers. Marker E-AAC/M-CTC(235) (AFLP8) had been previously converted into a SCAR. Work is in progress to convert the second of the linked markers, E-ACA/M-CTG(350), to a SCAR. The two linked AFLP markers converted to SCARs will be useful for developing yellow-seeded B. juncea lines by means of marker-assisted selection.

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The inheritance of seed colour and vernalization requirement in Brassica napus using doubled haploid populations

Cited by 32 publications

References 23 publications

Repression of seed coat pigmentation in Ethiopian mustard

Repression of seed coat pigmentation in Ethiopian mustard

Generation and mapping of SCAR and CAPS markers linked to the seed coat color gene in Brassica napus using a genome-walking technique

Mapping of AFLP markers linked to seed coat colour loci in Brassica juncea (L.) Czern

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