The infrastructure of aortic elastic fibers

Goldfischer, Sidney; Coltoff-Schiller, Bernice; Schwartz, Ernest; Blumenfeld, Olga O.

doi:10.1016/0040-8166(83)90074-5

Cited by 12 publications

(3 citation statements)

References 29 publications

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“…Clycogen, normally seen in the intraccllular sites, demonstrates discrete particulate PA-TCl-I-SP staining that is stronger than oxytalan libers and microfibrils. The observation of PA-TCH-SP staining of mierofibrils in the adventitia is consistent with previous ultrastructural cytocheinical of immunocytochemical studies demonstrating the presence of glycoproteins in aortic microfibrils (10,21), elastic cartilage perichondrium (12), and other tissue sites (22). The results corroborate previous biochemical studies ( [23][24][25][26][27] identifying glycoproteins associated with elastic tissue microfibrils.…”

Section: Discussionsupporting

confidence: 91%

Ultrastructural cytochemistry of oxytalan fibers in the periodontal ligament and microfibrils in the aorta with the periodic acid‐thiocarbohydrazide‐silver proteinate method

Takagi

Parmley

Yagasaki

et al. 1984

J Oral Pathology Medicine

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Fullmer's oxytalan fibers are special connective tissue fibers in periodontal ligaments and some non‐dental sites of certain animal species, and, ultrastructurally appear to resemble microfibrils related to clastogenesis. The present study has ultrastructurally examined the applicability of Thiéry's periodic acid‐thiocarbohydrazide‐silver proteinate (PA‐TCH‐SP) method for vicinal glycol‐containing complex carbohydrates to the study of oxytalan fibers in rat periodontal ligaments and microfibrils of the tunica adventitia in the rat aorta, where microfibrils are often associated with amorphous elastin and are thought to be oxytalan fibers. In the periodontal ligaments, the PA‐TCH‐SP method weakly to moderately stained collagen fibrils, moderately stained thin fibrils composing the oxytalan fibers, and intensely stained cytoplasmic granules of fibroblasts. In the aortic adventitia, the PA‐TCH‐SP method moderately stained collagen fibrils. Heavier staining was observed in microfibrils, whereas the amorphous elastin lacked staining. The most intense staining was seen in cytoplasmic granules and glycogen of mural cells. These studies demonstrate that oxytalan fibers in the periodontal ligament of rats and microfibrils in the aorta of rats contain vicinal glycol‐containing glycoproteins and the PA‐TCH‐SP method is a useful tool in ultrastructural studies of oxytalan fibers and microfibrils of rats.

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Section: Discussionsupporting

confidence: 91%

Ultrastructural cytochemistry of oxytalan fibers in the periodontal ligament and microfibrils in the aorta with the periodic acid‐thiocarbohydrazide‐silver proteinate method

Takagi

Parmley

Yagasaki

et al. 1984

J Oral Pathology Medicine

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“…Elastic fibers provide most of the elasticity of CT. Elastic fibers are differently organized depending on the tissue to form long, worm-like, thin fibers, fenestrated sheets (the tunica media of large elastic arteries and internal elastic lamina of small arteries), or coarse bundles in dermis and elastic cartilage [ 27 , 28 ]. Fibroblasts and vascular SMCs (VSMCs) synthesize all components of elastic fibers.…”

Section: Introductionmentioning

confidence: 99%

Reactive Oxygen Species and Oxidative Stress in the Pathogenesis and Progression of Genetic Diseases of the Connective Tissue

2020

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Connective tissue is known to provide structural and functional “glue” properties to other tissues. It contains cellular and molecular components that are arranged in several dynamic organizations. Connective tissue is the focus of numerous genetic and nongenetic diseases. Genetic diseases of the connective tissue are minority or rare, but no less important than the nongenetic diseases. Here we review the impact of reactive oxygen species (ROS) and oxidative stress on the onset and/or progression of diseases that directly affect connective tissue and have a genetic origin. It is important to consider that ROS and oxidative stress are not synonymous, although they are often closely linked. In a normal range, ROS have a relevant physiological role, whose levels result from a fine balance between ROS producers and ROS scavenge enzymatic systems. However, pathology arises or worsens when such balance is lost, like when ROS production is abnormally and constantly high and/or when ROS scavenge (enzymatic) systems are impaired. These concepts apply to numerous diseases, and connective tissue is no exception. We have organized this review around the two basic structural molecular components of connective tissue: The ground substance and fibers (collagen and elastic fibers).

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“…These properties are critical to the function of organs that undergo repeated cycles of extension and recoil, including arteries, lungs and skin [1]. Elastic fibres are made up of about 90% tropoelastin monomers and 10% microfibril proteins [2]. Impaired elastin production has been associated with several inherited and acquired conditions, such as stenosis of large arteries (Williams syndrome) [3], aortic aneurysms [4], emphysema [5], and loose, wrinkled skin (cutis laxa) [6].…”

Section: Introductionmentioning

confidence: 99%

Altered bladder function in elastin‐deficient mice at baseline and in response to partial bladder outlet obstruction

Jiang

Luttrell

et al. 2011

BJU International

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OBJECTIVE To examine functional and molecular changes of the bladders from elastin-haploinsufficient mice (Eln+/−) at baseline as well as in response to partial bladder outlet obstruction (pBOO). MATERIALS AND METHODS Female Eln+/− and wild type (Wt) mice (3–4 months old) were studied. The bladder elastin content was quantified by measuring desmosine. Mice were divided into two groups to undergo surgery to create pBOO or to undergo sham surgery. Three days after surgery, bladder function was evaluated by in vivo cystometry, and the contractile response of bladder strips exposed to electrical field stimulation (EFS) and carbachol was examined by ex vivo myography. RESULTS The Eln+/−-sham mice had a 33.6% decrease in bladder elastin compared with Wt-sham mice. Cystometry showed significantly decreased bladder compliance and capacity in Eln+/−-sham vs Wt-sham mice; pBOO increased bladder compliance and capacity to a greater extent in Eln+/− mice compared with Wt mice. Bladder strips from Eln+/−-sham mice showed a significantly heightened contractile response to both EFS and carbachol compared with Wt-sham mice. A significantly increased contractile response to carbachol was detected in Wt-pBOO vs Wt-sham but not between Eln+/−-pBOO and Eln+/−-sham mice. CONCLUSION The results that elastin-deficient mice had decreased bladder compliance and capacity and increased bladder contractility; and that Wt-pBOO mice showed an enhanced contractile response to carbachol, but Eln+/−-pBOO mice did not, suggest that elastin is critical for normal bladder function and is involved in bladder response to pBOO.

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The infrastructure of aortic elastic fibers

Cited by 12 publications

References 29 publications

Ultrastructural cytochemistry of oxytalan fibers in the periodontal ligament and microfibrils in the aorta with the periodic acid‐thiocarbohydrazide‐silver proteinate method

Ultrastructural cytochemistry of oxytalan fibers in the periodontal ligament and microfibrils in the aorta with the periodic acid‐thiocarbohydrazide‐silver proteinate method

Reactive Oxygen Species and Oxidative Stress in the Pathogenesis and Progression of Genetic Diseases of the Connective Tissue

Altered bladder function in elastin‐deficient mice at baseline and in response to partial bladder outlet obstruction

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