271 ( S . 4 ) , B -9 0 0 0 GENT (Belgium). R e c e i v e d 6 / 2 / 1 5 -A c c e p t e d 2 7 / 2 / 7 5 . SUMMARY solved into twenty-five fractions by preparative gas chromatography. Separation of the major compounds of these fractions was carried out by preparative open tubular column gas chromatography. The compounds were characterized by infrared, nuclear magnetic resonance, mass and ultraviolet spectroscopic studies. Trace constituents were investigated by combined gas chromatographymass spectrometry. Thirty-three compounds were identified, of which only six have been reported previously as occurring in black pepper oil. Nine compounds have to our knowledge never been found in natural products.The oxygen containing part of the essential oil of black pepper was re-NMR spectroscopy Russell and Jennings4' and Richard and Jennings6 identified linalool, 1-terpinen-4-01, a-terpineol, cryptone, carvone, p.cymen-6-01, transcarveol, cis-carveol, safrole, methyl eugenol, nerolidol, myristicin, cis and trans sabinene hydrate, cis-p. menthen-1-01, cis-p.-2,8-methandien-l-o1 and trans-pinocarveol . Sharma et al.3 re-RESULTS AND DISCUSSION The oxygenated fraction (10.0 % ) of black pepper oil was separated from the terpene hydrocarbons by column chromatography on silicagel'. gas chromatographic separations using Carbowax 20M as stationary phase divided the oxygenated fraction into twenty-five fractions as shown in fig.