The Influence of X-Rays on Organelle Induction and Differentiation in Grasshopper Spermatogenesis

Tahmisian, T. N.; Devine, R. L.

doi:10.1083/jcb.9.1.29

Cited by 31 publications

(19 citation statements)

References 6 publications

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“…Sometimes, the mitochondrial derivatives were disorganized, and could not extend parallel to the flagellar filaments in the tail region of the spermatids. Most of such abnormalities have also been reported by other workers (Mathur 1960, Tahmisian and Devine 1961, Cantacuzene and Seureau 1970. Two or sometimes more centriole adjuncts were observed to be attached to the nucleus in the treated spermatids.…”

Section: Cytoplasmic Organellessupporting

confidence: 59%

“…Supernumerary acrosomes, as reported by Tahmisian and Devine (1961) in the spermatids of the irradiated grasshopper, were not observed in the present study. However, the proacrosome was observed to elongate to give rise to acrosome at unusual places.…”

Section: Cytoplasmic Organellescontrasting

confidence: 48%

“…Supernumerary acrosomes, as described by Tahmisian and Devine (1961) in Melanoplus differentialis after irradiation, were not observed in the present study. Occasionally the acrosome in the treated grasshopper was not occupying its normal position at the anterior pole of the nucleus (Fig.…”

Section: Cytoplasmic Organellescontrasting

confidence: 48%

“…Supernumerary flagellar filaments, as observed in the treated material, are also reported to be induced by radiation in other grasshoppers (Mathur 1960, Tahmi sian andDevine 1961). The disorganization of the flagellar filaments, however, has not been reported in grasshoppers after treatment with sterilants or after ir radiation.…”

Section: Cytoplasmic Organellesmentioning

confidence: 79%

“…Mathur (1960), in his report on the effect of radiation with light microscopy, described four centriolar bodies on the posterior half of the nuclear membrane of spermatids. Tahmisian and Devine (1961) reported that after irradiation the centrioles at spermatogonium and spermatocyte stages were divided at least twice, resulting in four centrioles in one spermatid. According to them the number of the centrioles per spermatid was variable and the maximum number of such supernumerary centrioles was reported to be twenty.…”

Section: Cytoplasmic Organellesmentioning

confidence: 99%

See 4 more Smart Citations

Fine Structural Changes in the Spermatids of <i>Locusta migratoria</i> after the Administration of Isopropoxy Phosphine Oxide

Qureshi

1977

CYTOLOGIA

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Section: Cytoplasmic Organellessupporting

confidence: 59%

Section: Cytoplasmic Organellescontrasting

confidence: 48%

Section: Cytoplasmic Organellescontrasting

confidence: 48%

Section: Cytoplasmic Organellesmentioning

confidence: 79%

Section: Cytoplasmic Organellesmentioning

confidence: 99%

See 3 more Smart Citations

Fine Structural Changes in the Spermatids of <i>Locusta migratoria</i> after the Administration of Isopropoxy Phosphine Oxide

Qureshi

1977

CYTOLOGIA

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The number of centrioles in insect sperm: A study in two kinds of differentiating silkworm spermatids

Friedländer

Wahrman

1971

Journal of Morphology

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The typical eupyrene and atypical apyrene cycles of sperm differentiation in Bombyx mori were studied, with special attention to centriole number and behavior. Contrary to other reports, there is always only one centriole in the differentiating and in the mature sperm, thus confirming our previous findings that insect sperm has one centriole at the base of the flagellum, in contrast to two centrioles found in many other groups of animals. This numerical difference is discussed in an evolutionary context.We have previously suggested that insect spermatids have only one centriole, while those of many other animal groups contain two centrioles (Friedlander and Wahrman, '66). However, it has been claimed that a second centriole exists in insect spermatids as well (Anderson, '67; Breland, Barker, Eddleman and Biesele, '68; Phillips, ' 7 0 ) , or has been assumed to exist although it has not been observed (Shay and Biesele, '68).These conflicting opinions in the number of centrioles in insect spermatids should, if possible, be resolved before further investigations into the problem of centriole multiplication during spermiogenesis are attempted. We therefore set out to determine by electron microscopy the number of centrioles, and, if it occurs, their mode of replication in the spermatids of the silkworm Bombyx mori. Tanaka ('55), using light microscopy, reported in the same insect four and two centrioles at each pole of the first and second meiotic divisions respectively, and two centrioles in each spermatid. If this is correct, centrioles must replicate twice during meiosis.The silkworm (like other Lepidoptera) produces two types of spermatozoa, eupyrene and apyrene. Eupyrene spermatozoa have the haploid number of chromosomes, while apyrene ones have none. The fine structural changes during the differentiation of these two types of sperm were studied to verify whether the differences be-J. MORPH., 134: 383-398.tween them have anything to do with the number of centrioles. The whole cycle of sperm differentiation was carefully searched for a second centriole that might exist in spermatids, disappearing before sperm differentiation is completed. We have found only one centriole throughout the whole development of the two kinds of spermatozoa. MATERIAL AND METHODSThe silkworm moths belonged to a univoltine race of Bombyx mori reared in our laboratory.Eupyrene spermatogenesis takes place jn the larva and ceases in the pupa, while apyrene spermatogenesis starts in the pupa and continues in the adult (Tazima, '64). Testes were removed from the fifth larval instar for the study of the former, and from the adult moth for the investigation of the latter. The gonads were fixed with 1.7% glutaraldehyde in 0.1 M phosphate buffer (pH 7.3) for two hours, postfixed in 1% OsO, in veronal buffer (pH 7.3) at 4°C for one hour, and embedded in Epon 812. The ultrathin sections were mounted on naked grids, stained for six minutes in a 50% ethyl-alcohol solution saturated with uranyl acetate, covered with carbon and observed in an R...

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Comparative analysis of the neck region in spermatids and spermatozoa of some orthopteran insects

Guerra

Carballada

Esponda

1990

Journal of Morphology

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The ultrastructure of the neck region and the participation of noncovalent and disulphide bonds in the head-tail attachment were analyzed in spermatids and spermatozoa of some orthopteran species from the families Tettigoniidae and Acrididae. This study combined conventional electron microscopy with cytochemical procedures to detect acidic proteins and lysine-rich basic proteins, and with treatments using the disruptive agents sodium dodecyl sulfate (SDS) and dithiothreitol (DTT). The organization of the neck region differs in spermatozoa among species in the families analyzed. In Acrididae the neck consists of the beginning of the axoneme attached to the nuclear envelope and surrounded by the centriolar adjunct material. Tettigoniidae species possess a complex organization in the form of a laminar "connective piece," which shows a high content of lysine-rich and acidic proteins. Experiments using SDS and DTT demonstrate that in Acrididae the head-tail connection is strong, with both noncovalent and disulphide bonds important in maintenance of the attachment. In Tettigoniidae, however, despite the presence of a morphologically well organized connective piece, the head-tail attachment is biochemically labile in comparison to that of Acrididae. The relationships between the morphology of these neck structures and their stability mediated by noncovalent and/or disulphide bonds are discussed.

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The Influence of X-Rays on Organelle Induction and Differentiation in Grasshopper Spermatogenesis

Cited by 31 publications

References 6 publications

Fine Structural Changes in the Spermatids of <i>Locusta migratoria</i> after the Administration of Isopropoxy Phosphine Oxide

Fine Structural Changes in the Spermatids of <i>Locusta migratoria</i> after the Administration of Isopropoxy Phosphine Oxide

The number of centrioles in insect sperm: A study in two kinds of differentiating silkworm spermatids

Comparative analysis of the neck region in spermatids and spermatozoa of some orthopteran insects

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