The Influence of Unilateral Nephrectomy on the Development of Stilboestrol-induced Renal Tumours in the Male Hamster

Es, Horning

doi:10.1038/bjc.1954.68

Cited by 32 publications

(3 citation statements)

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“…Production of Tumours.-Renal carcinomas were induced in male golden hamsters by unilateral nephrectomy and subcutaneous implantation of a 15-mg. pellet of hexoestrol (Horning, 1954). When a tumour became palpable the hamster was killed by ether anaesthesia and tissues were taken as described for the rat hepatoma.…”

Section: Renal Carcinoma In Hamstersmentioning

confidence: 99%

Immunological Differences Between Normal and Malignant Cells

Nairn¹,

Richmond²,

McEntegart³

et al. 1960

BMJ

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[WITH SPECIAL PLATE]One of the traditional concepts of malignant change is that neoplastic cells lack certain growth-controlling enzyme systems present in normal cells. On this hypothesis, normal cells should contain proteins which are absent from malignant cells, and this difference in constitution should be demonstrable by immunological methods. Such an approach has been made by Weiler (1952), who compared the immunological properties of cytoplasmic particles from rat liver and from pnrmary hepatoma induced by 4-dimethylaminoazobenzene (D.A.B.). He described an organ-specific antigen present in normal liver cells, but apparently absent from tumour. By means of the fluorescent antibody method he showed (1956a) that the livers of D.A.B.-treated rats developed islands of cells in which the antigen concentration was reduced in comparison with the surrounding parenchyma-that is, there was diminished staining of these islands in histological sections treated with specific anti-liver serum conjugated with fluorescein. The degree of antigen impoverishment varied with the total dose of carcinogen and the duration of the experiment. These findings indicated a correlation between loss of antigen and carcinogenic change. Similar observations were made in subsequent studies (1956b, 1956c) of stilboestrol-induced carcinoma in hamsters. The work, which has been recently reviewed (Weiler, 1959), has not, so far as we are aware, been confirmed by other workers, though an unsuccessful attempt to repeat it is reported by Hughes et al. (1957).In the present investigation into the antigenic differences between normal tissues and tumours derived from them Weiler's findings in the D.A.B.-induced rat hepatoma have been corroborated. Similar results have also been obtained in preliminary studies of carcinoma in hamster kidney and of human skin tumours. MethodsLiver Carcinoma in RatsProduction of Tumours.-The method was based on that of Griffin et al. (1948). Male albino rats (Tuck strain), weighing 200-250 g., were fed ad lib. for six months on a low-protein diet containing 0.06% 4-dimethylaminoazobenzene or for ten weeks on the low-protein diet containing 0.08% 3'-methyl-4-dimethylamin-oazobenzene.When liver tumours became palpable the rats were killed by ether anaesthesia; thin blocks, each containing normal and tumour tissue, were " snap-frozen " in screw-cap bottles, pre-cooled in alcohol dry-ice freezing mixture, and stored at -200 C. Parallel blocks of tissue taken for conventional histological examination were fixed in formol-corrosive.Preparation of Antigen.-Finely chopped fresh rat liver was suspended (10% w/v) in chilled 0.25 M sucrose buffered at pH 7.3 (0.1 M phosphate). The suspension was homogenized for four minutes at about 10,000 r.p.m. in an M.S.E. homogenizer. Further cellular disintegration, controlled by microscopical examination, was accomplished by means of the M.S.E.-Mullard ultrasonic disintegrator. A total of 90 seconds sonic treatment was given, resulting in breakdown of about 80% of cells with dispersal of...

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Section: Renal Carcinoma In Hamstersmentioning

confidence: 99%

Immunological Differences Between Normal and Malignant Cells

Nairn¹,

Richmond²,

McEntegart³

et al. 1960

BMJ

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“…The induced tumors correspond, as for the renal tumors induced by carcinogens, with adenomas and renal adenocarcinomas [71][72][73][74][75][76]. Finally, other factors seem to accentuate renal carcinogenesis as unilateral nephrectomy [73,77] or chronic unilateral uretero-hydronephrosis (potentialization of the carcinogenic effects of DMN) [78], but these factors do not seem to directly induce the renal carcinogenesis process, strictly speaking.…”

Section: Animal Models Of Kidney Carcinomas Induced By Other Factorsmentioning

confidence: 99%

Heading Towards a Possible Rebirth of the Induced Renal Cell Carcinoma Models?

Mazzola¹,

Ribatti

2020

Cancers

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Introduction: Animal models are interesting tools to improve our knowledge of the pathophysiological processes underlying kidney cancer development. Recent advances have been made in the understanding of the genetic founding events underlying clear cell renal carcinoma. The aim of this paper was to review and discuss the characteristics of all the induced animal models of renal carcinogenesis that have been described in the scientific literature to date and to see if and how they could regain some use in the light of the latest discoveries. Methods: The authors reviewed all the papers available in PubMed regarding induced animal models of renal carcinogenesis. From this perspective, the keywords “induced”, “animal model”, and “renal cancer” were used in PubMed’s search engine. Another search was done using the keywords “induced”, “animal model”, and “kidney cancer”. PRISMA recommendations were used to develop the literature review. Results: Seventy-eight studies were included in this review. Results were presented depending on the mechanisms used to induce carcinogenesis in each model: induction by carcinogens, hormones, viral induction, or induction by other agents. Discussion focused on the possibility to rethink these different induced animal models and use them to answer new research questions. Conclusion: Many induced animal models have been developed in the past to study renal cancer. While these models seemed unable to yield new knowledge, the latest advances in the understanding of the genetics behind renal carcinogenesis could well bring the models back to the forefront.

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“…Homing noted that renal carcinomas arose more rapidly in Stilbestrol-treated nephrectomized hamsters than in similarly treated unoperated on controls-possibly because the single kidney is less able to deal effectively with the elimination of estrogen metabolites. 31 Rosen and ,Cole reported the occurrence of renal tumors in x-irradiated mice to be greatly increased "as a consequence of the interaction of the specific proliferative stimulus of unilateral nephrectomy on radiation altered kidney celW.64…”

Section: Kidneymentioning

confidence: 99%