The influence of ultra-low temperatures on marine microalgal cells

Boroda, Andrey V.; Айздайчер, Н. А.; Odintsova, N. A.

doi:10.1007/s10811-013-0093-5

Cited by 13 publications

(9 citation statements)

References 63 publications

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“…However, other strains showed very high flexibility and tolerance to both cryopreservation techniques tested such as Chlorocystis sp., Mychonastes sp., and Nannochloris sp., showing fast recovery even after 1 year of incubation in the liquid nitrogen. These results confirmed the findings of Abreu et al (2012), confirming the difficulty in establishing common protocols for different microalgae species since cryo-sensitivity of marine algae resulting from the differences in natural intrinsic characteristics rather than their taxonomic position (Boroda et al 2014). Cryoprotectant nature and concentration also showed an effect on the viability of the different strains.…”

Section: Resultssupporting

confidence: 81%

Cryopreservation of microalgae from desert environments of Qatar

et al. 2015

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The Qatar University Culture Collection of Cyanobacteria and Microalgae (QUCCCM) is a unique resource containing a diverse collection of microalgae and cyanobacteria, isolated from the Qatar desert environment. In order to ensure maximum preservation of this resource, a number of cryopreservation techniques were applied to various strains, and the preservation effectiveness (cell viability and lipid productivity) was determined. The conditions tested were direct, passive, and freeze-cooling cryopreservation (technique), dimethyl sulfate and methanol (cryoprotectant), and 5 and 10 % cryoprotectant concentrations over storage durations of up to 1 year. It was shown that the cryopreservation regime is strain dependent, and strains belonging to the same genera can have different requirements. On the other hand, neutral lipid estimation, via Nile red fluorescence determination of pre-and post-cryopreserved microalgae isolates, confirmed that the lipid production is affected by the applied cryopreservation method.

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Section: Resultssupporting

confidence: 81%

Cryopreservation of microalgae from desert environments of Qatar

et al. 2015

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“…Our experiments suggest that cell characteristics which may have evolved as an adaption to environmental characteristics could play a major role in determining whether a diatom species (or strain) is able to survive ultra-low temperatures and that cryopreservation success could largely depend on the species ecology (habitat type). In this context, cell wall properties such as the amount of cell-wall associated polysaccharides or the degree of cell wall silicification could play an important role 32 . Differences in cell size might be another factor explaining intraspecific differences in cryopreservation success 44 .…”

Section: Discussionmentioning

confidence: 99%

“…Freshwater diatoms, for instance, have proven particularly difficult to cryopreserve 29 . In view of the growing interest in diatoms, both within fundamental and applied research, there is a pressing need to improve existing cryopreservation protocols 30 – 32 .…”

Section: Introductionmentioning

confidence: 99%

Expanding the toolbox for cryopreservation of marine and freshwater diatoms

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Pinseel

Decker

et al. 2018

Sci Rep

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Diatoms constitute the most diverse group of microalgae and have long been recognised for their large biotechnological potential. In the wake of growing research interest in new model species and development of commercial applications, there is a pressing need for long-term preservation of diatom strains. While cryopreservation using dimethylsulfoxide (DMSO) as a cryoprotective agent is the preferred method for long-term strain preservation, many diatom species cannot be successfully cryopreserved using DMSO. Therefore, in this study, we studied cryopreservation success in six different diatom species, representing the major morphological and ecological diatom groups, using a range of DMSO concentrations and Plant Vitrification Solution 2 (PVS2) as an alternative cryoprotectant to DMSO. In addition, we tested whether suppressing bacterial growth by antibiotics accelerates the post-thaw recovery process. Our results show that the effects of cryoprotectant choice, its concentration and the addition of antibiotics are highly species specific. In addition, we showed that PVS2 and antibiotics are useful agents to optimize cryopreservation of algae that cannot survive the traditional cryopreservation protocol using DMSO. We conclude that a species-specific approach will remain necessary to develop protocols for diatom cryopreservation and to increase their representation in public culture collections.

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“…Boroda et al (2014) reported that the red alga Porphyridium purpureum (Bory de Saint-Vincent) K.M. Drew & R. Ross showed no discernible growth for 5-6 days after thawing from cryopreservation at −196°C, but recovered to grow faster than control cultures.…”

Section: Tablementioning

confidence: 99%