The influence of time elapsed after milking and lactation stage on the lactoperoxidase system of milk in Sahiwal cattle and Nili‐Ravi buffaloes

Abstract

The effects of time elapsed after milking and lactation stage were assessed on the lactoperoxidase (LP) enzyme activity and concentrations of thiocyanate in Sahiwal cattle (N = 15) and Nili‐Ravi buffaloes (N = 15). The LP activity and thiocyanate concentrations decreased as the time elapsed after milking (P < 0.05) but the concentration of these components increased as the lactation advanced. The LP enzyme activity was sufficient until 12 h post milking but the thiocyanate concentration after 8 h decreased to an insufficient level to activate the LP System, hence additional thiocyanate would be beneficial from external sources. No difference was noted regarding the LPS activity in cattle and buffaloes at the time of collection and subsequently after storage. However, at the end of lactation, the thiocyanate concentration was lower in buffaloes than that in cattle. (P < 0.05).

Lactoperoxidase enzyme activity and thiocyanate levels in raw milk of Girolando cows

“…The thiocyanate levels obtained in this study were higher than those found by Fweja et al (2007) in milk of Ayrshire and Tanzania Short Horn Zebu cows, and by Ponce (2012) in animals of a rustic breed in Cuba, Venezuela, and Mexico. The thiocyanate levels also varied between the groups, corroborating the results found by Ponce et al (1998), Fonteh et al (2002), Seifu et al (2007), and Yaqub et al (2012).…”

“…The mean activity of the lactoperoxidase found in this study was higher than those observed in studies carried out with Holstein cows in Iran (Asadpour et al, 2008) and in Italy (Calamari et al, 2005). The lactoperoxidase activity had a high variation when comparing the samples, corroborating the results found by Seifu et al (2007), Lujerdean and Buena (2010), and Yaqub et al (2012).…”

“…The production level, although it has had no influence on the lactoperoxidase activity, could alter milk composition because the content of each milk components is not necessarily related to the increase or decrease in the volume of milk produced. However, Yaqub et al (2012) found no association between lactoperoxidase activity and milk production.…”

“…Regarding the number of days in lactation, Althaus et al (2001), Fonteh et al (2002), Lujerdean and Buena (2010), and Yaqub et al (2012), studying milk of sheep, goat, cow and buffalo, respectively, found a cyclic variation in lactoperoxidase activity, with increases during lactation and subsequent reduction. The production level, although it has had no influence on the lactoperoxidase activity, could alter milk composition because the content of each milk components is not necessarily related to the increase or decrease in the volume of milk produced.…”