The influence of the surface properties of silicon–fluorine hydrogel on protein adsorption

Xie, Haijiao; Zhao, Zheng‐Bai; An, Shuangshuang; Jiang, Yong

doi:10.1016/j.colsurfb.2015.11.027

Cited by 11 publications

(8 citation statements)

References 33 publications

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“…According to the isoelectric point (PI) theory, the isoelectric points of BSA and LZM are 4.7 and 11.0 to 13.5 respectively. Therefore, in PBS solution at pH 7.4, BSA is negatively charged while LZM is positively charged . Figure demonstrates that hydroxyl and carboxylate functional groups are generated on the PDMS film surface after hydrophilic reversal treatment, which results in the modified PDMS surface being negatively charged.…”

Section: Resultsmentioning

confidence: 96%

“…Static adsorption method was used to test protein adsorption . Bovine serum albumin (BSA), LZM, and SDS solutions prepared with pH 7.4 PBS buffer solution were used to evaluate protein adsorption.…”

Section: Methodsmentioning

confidence: 99%

“…Therefore, in PBS solution at pH 7.4, BSA is negatively charged while LZM is positively charged. 27 Figure 3 demonstrates that hydroxyl and carboxylate functional groups are generated on the PDMS film surface after hydrophilic reversal treatment, which results in the modified PDMS surface being negatively charged. In addition, the ATR-FTIR spectrum shows that the peak intensity of the carboxylate groups on the surface of the modified PDMS is significantly enhanced with the increase of the TMSEMA content, which explains BSA relative adsorption ratio decrease with increasing TMSEMA content while that of LZM increases.…”

Section: Evaluation Of Protein Adsorptionmentioning

confidence: 99%

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Reverse hydrophobic PDMS surface to hydrophilic by 1‐step hydrolysis reaction

Zhang

Kalulu

Wang

et al. 2018

Polymers for Advanced Techs

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Hydrophilic modification on the surface of polymer polydimethylsiloxane (PDMS) material is a key step for its application in biomaterial, bioengineering, and so on. In this article, a novel and effective method was proposed to reverse hydrophobic surface to hydrophilic by 1‐step hydrolysis of Si―O bond to produce hydrophilic hydroxyl group. The hydrophilizing reagent 2‐(trimethylsiloxy) ethyl methacrylate (TMSEMA) was used during the copolymerization of polydimethylsiloxane prepolymer (DMS U21). The prepared PDMS film was subjected to 1‐step surface hydrophilic reversal treatment using KOH solution to produce hydroxyl groups on the surface. The contact angle, attenuated total reflection Fourier transform infrared spectra, and equilibrium water content (EWC) measurements were conducted on PDMS films. The results showed that TMSEMA content had no obvious impact on the contact angle and EWC value of untreated PDMS. After reversal treatment, the contact angle decreased from 94° to 15°, and the EWC value increases to 10% when the TMSEMA content was 15 wt%. The spectrum proved that the reverse reaction produced hydroxyl and carboxylate on the surface. The hydrophilic stability, surface morphology, and protein adsorption properties of PDMS film were also investigated. This study can provide new ideas and further reference for improving the hydrophilicity of PDMS surface.

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Section: Resultsmentioning

confidence: 96%

Section: Methodsmentioning

confidence: 99%

Section: Evaluation Of Protein Adsorptionmentioning

confidence: 99%

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Reverse hydrophobic PDMS surface to hydrophilic by 1‐step hydrolysis reaction

Zhang

Kalulu

Wang

et al. 2018

Polymers for Advanced Techs

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“…Therefore, the ratio of AA and NIPAM would be one of the vital parameters in synthesizing the dual sensitivity of hydrogel, and it is necessary to be further investigated. Furthermore, another appropriate approach for enhancing the swelling capacity of hydrogel is to modify the hydrogel with inorganic materials, such as silicon 15,16 or silica 17,18 . It can improve the swelling performance and simultaneously increase the mechanical strength of the hydrogel 19 .…”

Section: Introductionmentioning

confidence: 99%

Synthesis of dual pH‐ and temperature‐sensitive poly(N‐isopropylacrylamide‐co‐acrylic acid)/sewage sludge ash hydrogel with the simultaneously high performance of swelling and deswelling

Pan

Huang

Guo

et al. 2021

Polymers for Advanced Techs

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An advanced dual pH‐ and temperature‐sensitive hydrogel (NASH2.5) was optimally synthesized through modification of N‐isopropylacrylamide (NIPAM) hydrogel with introducing 5 mol% acrylic acid (AA) and 2.5 wt% sewage sludge ash (SSA). The swelling kinetic results showed that NASH2.5 exhibited both high equilibrium swelling ratio and swelling rate, which was attributed to the higher porous structure as shown in scanning electron microscope, and the more hydrogen bonding formed inside of the hydrogel as investigated in Fourier transform infrared spectrometer. In addition, its curve was better fitted to the pseudo‐second‐order model, indicating that the water absorption process was dominated by chemisorption through forming the hydrogen bonding among the water molecules and carboxyl/silanol groups of the hydrogel. Compared with the pure NIPAM hydrogel, the water transport mechanism switched from Case I diffusion to Case II diffusion by introduction of AA and further SSA. Furthermore, through the results of the deswelling kinetics in pH value change (from 9 to 4 and 2, respectively), temperature value change (from 25 to 40, 50, and 60°C, respectively), and dual pH and temperature values changes, NASH2.5 not only presented a high pH sensitivity, but also showed high sensitive to temperature by achieving high water recovery ratio in rapid dehydrated rate. Therefore, the dual stimuli‐sensitive hydrogel with the simultaneously high performance of swelling and deswelling would provide a suitable alternative for specific applications such as pollutant adsorption.

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“…Due to its hydrophobicity, PDMS has been used without any surface treatment as substrate for spheroid growth [16], even with the drawback that a high hydrophobicity and porosity may allow an unspecific absorption/adsorption of small molecules [33] and alter the cell culture conditions. To reduce this undesired effect, some coatings with fluoro-compounds can be used because of their strongly biofouling properties [34][35][36]. To assemble fluorinated coatings on PDMS, we recently exploited a strategy based on Fluorolink ® F10 (referred in the text as F10) in the presence of a protein, the hydrophobin HFBII, which acts as a primer and creates a biocompatible, grafted oleophobic coating [37,38].…”

Section: Introductionmentioning

confidence: 99%

Lab-on-a-brane for spheroid formation

et al. 2019

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The influence of the surface properties of silicon–fluorine hydrogel on protein adsorption

Cited by 11 publications

References 33 publications

Reverse hydrophobic PDMS surface to hydrophilic by 1‐step hydrolysis reaction

Reverse hydrophobic PDMS surface to hydrophilic by 1‐step hydrolysis reaction

Synthesis of dual pH‐ and temperature‐sensitive poly(N‐isopropylacrylamide‐co‐acrylic acid)/sewage sludge ash hydrogel with the simultaneously high performance of swelling and deswelling

Lab-on-a-brane for spheroid formation

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