The influence of the sensory neurotransmitter calcitonin gene-related peptide on bone marrow mesenchymal stem cells from ovariectomized rats

Abstract

In order to explore the effects of calcitonin gene-related peptide (CGRP) on bone mesenchymal stem cells (BMSCs) from ovariectomized (OVX) rats, an OVX rat model was used. An ELISA was performed to examine the changes in CGRP level in the plasma and skeleton. The BMSCs from the sham rats were designated group A. The BMSCs from the OVX rats (groups B, C, D and E) were treated with different concentrations of CGRP (10, 10, 10 and 0 M) in vitro. The proliferation and osteogenic and adipogenic differentiation potential of the BMSCs were evaluated. BMSCs sheets and Bio-Oss mixtures were transplanted into nude mice to observe the effects of CGRP on bone formation in vivo. The level of CGRP was decreased by almost 27 and 17 % in the plasma and bone, respectively, in OVX rats compared with sham rats (p < 0.05). Treatment with CGRP increased the proliferation and mineralization of BMSCs, and significantly decreased the lipid accumulation of BMSCs in a dose-dependent manner. The expression of Runx2 and Osterix was upregulated, but the expression of peroxisome proliferator-activated receptor γ was significantly downregulated in groups B, C and D compared with group E (p < 0.05). Micro computed tomography showed no difference between the images of the planted mixtures. Hematoxylin and eosin stain revealed the formation of slightly more hard bone-like structures in groups B and C. These results suggested that CGRP played a role in adjusting bone mass and strength by promoting the proliferation and osteogenic differentiation of BMSCs, as well as significantly suppressing the adipogenic differentiation of BMSCs.

Mesenchymal Stem Cells in Synovial Fluid Increase in Knees with Degenerative Meniscus Injury after Arthroscopic Procedures through the Endogenous Effects of CGRP and HGF

“…(32) Chen et al reported that 10 − 6 -10 − 10 M CGRP promoted proliferation of bone marrow MSCs in a dose-dependent manner and that CGRP played a role in adjusting bone mass and strength in ovariectomized rats. (33) In the current study, HGF signal intensity was also correlated with colony number per dish in 7 donors, and HGF promoted migration of synovial uid MSCs. HGF is secreted by mesenchymal cells and has effects for morphogenesis, angiogenesis, in ammation, tissue repair, etc.…”

“…In addition, these factors have already been reported to promote the migration of MSCs, though they were not found to derive from synovium. (32,33) This suggests that other factors that showed a high relationship between the MSC number and protein concentration in the synovial uid might also promote the migration of synovial uid MSCs in the migration assay we performed.…”

Effect of capsaicin-sensitive sensory neurons on bone architecture and mechanical properties in the rat hindlimb suspension model

“…Unmyelinated sensory neurons contain a variety of transmitter peptides, such as CGRP and SP. CGRP has been reported to inhibit osteoclastogenesis [36] and bone resorption [37] and to stimulate osteoblasts proliferation and bone formation [38] , [39] . SP could stimulate osteoclast resorption and is involved in the pathogenesis of bone changes [40] .…”

Effect of Different Doses of Capsaicin Administration and Titanium Implant Osseointegration

“…Zhang et al reported that capsaicin-sensitive sensory nerve fibers were CGRP-positive nerve fibers [17]. In addition, it has been reported that when regular capsaicin treatment is applied CGRPpositive nerve fibers are depleted, bone formation is inhibited and bone resorption is increased [35].…”