The influence of the deletion on the long arm of the Y chromosome on sperm motility in mice

Grzmil, Paweł; Gołas, Aniela; Müller, Christian; Styrna, Józefa

doi:10.1016/j.theriogenology.2006.10.007

Cited by 28 publications

(18 citation statements)

References 26 publications

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“…Estimated levels of impairment: 5 not affected; * slightly affected; ** moderately affected; *** severely affected; **** very severely affected. The levels of phenotypic effects of NPYq/Sly-deficient mice presented in this table are based on the present study and on our unpublished data, as well as on several previously published manuscripts from our group and few others Ellis et al, 2005;Grzmil et al, 2007;Reynard et al, 2007;Styrna et al, 2002;Styrna et al, 2003;Touré et al, 2005;Touré et al, 2004;Ward and Burgoyne, 2006;Xian et al, 1992;Yamauchi et al, 2010;Yamauchi et al, 2009) and therefore should be considered an estimate. retained histones and histone modifications (Brykczynska et al, 2010;Hammoud et al, 2009), and that disturbance of this epigenetic imprint during the remodeling could have clinically important consequences for offspring (de Boer et al, 2010;Ng et al, 2010).…”

Section: Discussionmentioning

confidence: 68%

Deficiency of the multi-copy mouse Y gene Sly causes sperm DNA damage and abnormal chromatin packaging

Riel

Yamauchi

Sugawara

et al. 2012

Journal of Cell Science

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SummaryIn mouse and man Y chromosome deletions are frequently associated with spermatogenic defects. Mice with extensive deletions of nonpairing Y chromosome long arm (NPYq) are infertile and produce sperm with grossly misshapen heads, abnormal chromatin packaging and DNA damage. The NPYq-encoded multi-copy gene Sly controls the expression of sex chromosome genes after meiosis and Sly deficiency results in a remarkable upregulation of sex chromosome genes. Sly deficiency has been shown to be the underlying cause of the sperm head anomalies and infertility associated with NPYq gene loss, but it was not known whether it recapitulates sperm DNA damage phenotype. We produced and examined mice with transgenically (RNAi) silenced Sly and demonstrated that these mice have increased incidence of sperm with DNA damage and poorly condensed and insufficiently protaminated chromatin. We also investigated the contribution of each of the two Sly-encoded transcript variants and noted that the phenotype was only observed when both variants were knocked down, and that the phenotype was intermediate in severity compared with mice with severe NPYq deficiency. Our data demonstrate that Sly deficiency is responsible for the sperm DNA damage/chromatin packaging defects observed in mice with NPYq deletions and point to SLY proteins involvement in chromatin reprogramming during spermiogenesis, probably through their effect on the post-meiotic expression of spermiogenic genes. Considering the importance of the sperm epigenome for embryonic and fetal development and the possibility of its inter-generational transmission, our results are important for future investigations of the molecular mechanisms of this biologically and clinically important process.

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Section: Discussionmentioning

confidence: 68%

Deficiency of the multi-copy mouse Y gene Sly causes sperm DNA damage and abnormal chromatin packaging

Riel

Yamauchi

Sugawara

et al. 2012

Journal of Cell Science

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“…In mice, deficiency in MSYq transcripts deriving from mousespecific multicopy genes causes abnormalities in the sperm heads, and MSYq deletion mutants display a range of teratozoospermia and infertility phenotypes with severities corresponding to the extent of the deletion (23,25,26,(31)(32)(33). The increase in flat sperm heads is specific to MSYq deletions in mice, and even minor changes in the sperm head structure can affect sperm motility and thereby fertility (26,32,33). Accordingly, the mild but consistent head defects found in the Hsf2 Ϫ/Ϫ sperm (Fig.…”

Section: Discussionmentioning

confidence: 99%

Promoter ChIP-chip analysis in mouse testis reveals Y chromosome occupancy by HSF2

Åkerfelt

Henriksson

Laiho

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

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The mammalian Y chromosome is essential for spermatogenesis, which is characterized by sperm cell differentiation and chromatin condensation for acquisition of correct shape of the sperm. Deletions of the male-specific region of the mouse Y chromosome long arm (MSYq), harboring multiple copies of a few genes, lead to sperm head defects and impaired fertility. Using chromatin immunoprecipitation on promoter microarray (ChIP-chip) on mouse testis, we found a striking in vivo MSYq occupancy by heat shock factor 2 (HSF2), a transcription factor involved in spermatogenesis. HSF2 was also found to regulate the transcription of MSYq resident genes, whose transcriptional regulation has been unknown. Importantly, disruption of Hsf2 caused a similar phenotype as the 2/3 deletion of MSYq, i.e., altered expression of the multicopy genes and increased mild sperm head abnormalities. Consequently, aberrant levels of chromatin packing proteins and more frequent DNA fragmentation were detected, implying that HSF2 is required for correct chromatin organization in the sperm. Our findings define a physiological role for HSF2 in the regulation of MSYq resident genes and the quality of sperm. chromatin packing ͉ heat shock factor ͉ MSYq ͉ promoter microarray ͉ spermatogenesis

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“…Analyses were carried out with logtransformed variables. Data for straightline velocity (VSL) were obtained from the literature (Mortimer and Mortimer, 1990;Stachecki et al, 1993Stachecki et al, , 1994Shivaji et al, 1995;Mahonynet al, 1996;Moore and Akhondi, 1996;Trimeche et al, 1996Trimeche et al, , 1999Patil et al, 1998;van der Horst et al, 1999;Castellini et al, 2000Castellini et al, , 2006Jayaprakash et al, 2001;Kawaguchi et al, 2004;Lavara et al, 2005;Miró et al, 2005;Baumber and Meyers, 2006;Cox et al, 2006;Sloter et al, 2006;Aurich et al, 2007;Grzmil et al, 2007;Holt et al, 2007;Hung et al, 2007;Immler et al, 2007a;Rijsselaere et al, 2007) and our own unpublished observations. Data for total sperm length were obtained from Cummins and Woodall (1985), Cassinello et al (1998), Gage and Freckelton (2003) and Malo et al (2006 Pitnick et al, 2001).…”

Section: ) (C)mentioning

confidence: 99%

Implications of diversity in sperm size and function for sperm competition and fertility

Gomendio

Roldán²

2008

Int. J. Dev. Biol.

167

185

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Sperm competition is now recognised as a potent selective force shaping many male reproductive traits. While the influence of sperm competition on sperm number is widely accepted, its effects upon sperm size remain controversial. It had been traditionally assumed that there is a trade-off between sperm number and sperm size, so that an increase in sperm number would result in a decrease in sperm size, under conditions of sperm competition. Contrary to this prediction, we proposed some time ago that sperm competition favours an increase in sperm size, because longer sperm swim faster and are more likely to win the race to fertilize ova. Comparative studies between species show that in many taxa such a relationship exists, but the consequences of an increase in sperm size may vary between taxa depending on the environment in which spermatozoa have to compete. We present new evidence showing that in mammals longer sperm swim at higher speeds. We also show that mean swimming speed is highly correlated with maximum swimming speed, so even if the fastest swimming sperm are more likely to fertilize, both measures are informative. When individuals of the same species are compared, ratios between the dimensions of different sperm components, as well as the shape of the head, seem better at explaining sperm swimming velocity. Finally, we show that mean and maximum sperm swimming speed determine male fertility. Other studies have shown that in competitive contexts, males with faster swimming sperm have higher fertilization success. We conclude that the available evidence supports our original hypothesis.

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The influence of the deletion on the long arm of the Y chromosome on sperm motility in mice

Cited by 28 publications

References 26 publications

Deficiency of the multi-copy mouse Y gene Sly causes sperm DNA damage and abnormal chromatin packaging

Deficiency of the multi-copy mouse Y gene Sly causes sperm DNA damage and abnormal chromatin packaging

Promoter ChIP-chip analysis in mouse testis reveals Y chromosome occupancy by HSF2

Implications of diversity in sperm size and function for sperm competition and fertility

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