The influence of the corpus luteum on metabolites composition of follicular fluid from different sized follicles and their relationship to serum concentrations in dairy cows

Shabankareh, H. Karami; Kor, Nasroallah Moradi; Hajarian, Hadi

doi:10.1016/j.anireprosci.2013.06.018

Cited by 20 publications

(9 citation statements)

References 39 publications

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“…Physically, the content of lipid metabolites in FF fluctuates in accordance with the whole system level since FF is indeed an exudate of peripheral blood (47). However, several studies maintained the opposite notion (48). In order to find the source of RvE1 in FF, we collected samples of FF and serum from the same patient on the day of oocyte retrieval and detected RvE1's concentration by LC/ESI-MS/MS.…”

Section: Discussionmentioning

confidence: 99%

Resolvin E1 in Follicular Fluid Acts as a Potential Biomarker and Improves Oocyte Developmental Competence by Optimizing Cumulus Cells

Zhang

Zhu

et al. 2020

Front. Endocrinol.

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Metabolic profile of follicular fluid (FF) has been investigated to look for biomarkers for oocyte quality. Resolvin E1 (RvE1), a potent pro-resolving mediator, was reported to have protective action in cell function. The study aimed to examine the predictive value of RvE1 for oocyte quality and to explore the cellular mechanism of RvE1 in improving oocyte competence. Metabolic profiles of 80 FF samples showed a higher level of RvE1 in group A (blastocysts scored ≥ B3BC and B3CB according to Gardner's blastocyst scoring system, N = 36) than that of group B (blastocysts scored < B3BC and B3CB, N = 44, P = 0.0018). The receiver operating characteristic (ROC) curve analysis showed that RvE1 level in FF below 8.96 pg/ml (AUC:0.75; 95%CI: 0.64-0.86; P = 0.00012) could predict poor oocyte quality with specificity of 97.22%, suggesting RvE1 as a potential biomarker to exclude inferior oocytes. Besides, the level of RvE1 was found to be significantly lower in FF than in serum (57.49 to 17.62 pg/ml; P = 0.0037) and was gradually accumulated in the culture medium of cumulus cells (CCs) during cell culture, which indicated that RvE1 came from both blood exudates and local secretion. The in vitro experiment revealed the cellular mechanism of RvE1 in improving oocyte quality by decreasing the cumulus cell apoptotic rate and increasing cell viability and proliferation. It is the first time that the role of RvE1 in reproduction is explored. In conclusion, RvE1 is valuable as a potential exclusive biomarker for oocyte selection and plays a role in improving oocyte quality.

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Section: Discussionmentioning

confidence: 99%

Resolvin E1 in Follicular Fluid Acts as a Potential Biomarker and Improves Oocyte Developmental Competence by Optimizing Cumulus Cells

Zhang

Zhu

et al. 2020

Front. Endocrinol.

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“…Furthermore, recovery of ovaries was performed according to the standards, and ovaries were not categorized on the basis of the presence or absence of the CL. Follicles with redcolored fluid and atretic characteristics were not aspirated too [19]. Therefore, some individual physiological and pathologic conditions that could affect the ovarian function and could interfere with the study of side of ovaries local effects were considered or not included in this study.…”

Section: Oocyte Collectionmentioning

confidence: 99%

In vivo and in vitro study of the function of the left and right bovine ovaries

et al. 2015

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“…In addition, preovulatory FF in which the maturation naturally occurs may sustain a better cytoplasmic maturation and thus a better competence for in vitro fertilisation (IVF) and development [44]. In bovines, the developmental potential of oocytes originating from CL-bearing and non-CL-bearing ovaries has been evaluated [45]. The percentage of blastocyst formation of oocytes originating from the medium and large follicles of non-CL-bearing ovaries was greater than that of oocytes originating from the small and medium follicles of CL-bearing ovaries.…”

Section: Discussionmentioning

confidence: 99%

Can the Presence of Ovarian Corpus Luteum Modify the Hormonal Composition of Follicular Fluid in Mares?

Satué

Fazio

Medica

2020

Animals

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The hypothesis of this study was to investigate if the presence of corpus luteum (CL) in one ovary could modify the hormonal content of follicular fluid (FF) in the follicles. Sixty ovaries were taken after the slaughter of 30 clinically healthy mares. In relation to the sizes, the follicles were classified into three different categories, as small (20–30 mm), medium (31–40 mm) and large (≥41 mm). Blood samples were collected from the jugular vein of mares before their slaughter, and then the FF samplings were extracted from each single follicle. The ovaries that were collected were classified into two groups, according to the presence (CL-bearing) or absence (non-CL-bearing) of CL. The serum and FF samples were analysed for progesterone (P4), oestradiol-17β (E2), testosterone (T), androstenedione (A4) and dehydroepiandrosterone (DHEA). Intrafollicular P4 concentrations in large follicles of CL-bearing groups were lower than for non-CL-bearing ones. Intrafollicular E2 concentrations increased with the increase of the follicle diameter in both groups, CL-bearing and non-CL-bearing. However, in the FF with a large and medium follicle size, E2 concentrations were significantly higher in non-CL-bearing groups than in CL-bearing groups. T and A4 significantly increased in the large and medium follicle sizes when compared to the small follicle sizes in both groups, but higher concentrations in the non-CL-bearing group were obtained. Intrafollicular DHEA significantly decreased with the increase of the follicular diameter in both groups. Steroid hormones in FF dynamically changed, according to the presence or not of CL in the ovary. This study brings new knowledge on the role of the CL in the follicular hormonal composition in mares.

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The influence of the corpus luteum on metabolites composition of follicular fluid from different sized follicles and their relationship to serum concentrations in dairy cows

Cited by 20 publications

References 39 publications

Resolvin E1 in Follicular Fluid Acts as a Potential Biomarker and Improves Oocyte Developmental Competence by Optimizing Cumulus Cells

Resolvin E1 in Follicular Fluid Acts as a Potential Biomarker and Improves Oocyte Developmental Competence by Optimizing Cumulus Cells

In vivo and in vitro study of the function of the left and right bovine ovaries

Can the Presence of Ovarian Corpus Luteum Modify the Hormonal Composition of Follicular Fluid in Mares?

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