The influence of temperature on plant development in a vernalization-requiring winter wheat: A 2-DE based proteomic investigation

Rinalducci, Sara; Egidi, Maria Giulia; Mahfoozi, S.; Godehkahriz, Sodabeh Jahanbakhsh; Zolla, Lello

doi:10.1016/j.jprot.2011.02.005

Cited by 56 publications

(46 citation statements)

References 61 publications

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“…These positional variations may be due to protein post-translational modifications (PTMs), different copies of a same gene or protein degradation [26]. Similar positional variations have also been reported in previous studies [27,28]. In addition, some of the specific spots were detected in more than one protein identification (Supplementary Table 3), which were possibly of comigration of multiple proteins [29].…”

Section: Discussionsupporting

confidence: 79%

Physiology and proteomics research on the leaves of ancient Platycladus orientalis (L.) during winter

Zhang

Chai

et al. 2015

Journal of Proteomics

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Section: Discussionsupporting

confidence: 79%

Physiology and proteomics research on the leaves of ancient Platycladus orientalis (L.) during winter

Zhang

Chai

et al. 2015

Journal of Proteomics

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“…In Escherichia coli, a prokaryotic model of the cold shock response, a shift in temperature from 37°C to 10°C shows that cells specifically overexpress CSPs over a period of approximately 4 h, while the global transcription and translation rates decrease (Gualerzi et al, 2003). This is in sharp contrast to what has been observed in plants, where cold stress is accompanied by alterations in protein synthesis principally targeting photosynthesis and carbohydrate metabolism pathways (Yan et al, 2006;Neilson et al, 2011;Rinalducci et al, 2011aRinalducci et al, , 2011b. Intriguingly, CSPs are the most abundant nucleic acid binding domain found in Lingulodinium polyedrum (Beauchemin et al, 2012) and Symbiodinium spp.…”

Section: Discussionmentioning

confidence: 88%

Cold-Induced Cysts of the Photosynthetic DinoflagellateLingulodinium polyedrumHave an Arrested Circadian Bioluminescence Rhythm and Lower Levels of Protein Phosphorylation

2013

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Dinoflagellates are microscopic, eukaryotic, and primarily marine plankton. Temporary cyst formation is a well-known physiological response of dinoflagellate cells to environmental stresses. However, the molecular underpinnings of cold-induced cyst physiology have never been described. Cultures of the photosynthetic dinoflagellate Lingulodinium polyedrum readily form temporary cysts when placed at low (8°C 6 1°C) temperature and excyst to form normal motile cells following a return to normal temperature (18°C 6 1°C). The normal circadian bioluminescence rhythm and the expected changes in Luciferin Binding Protein abundance were arrested in L. polyedrum cysts. Furthermore, after excystment, the bioluminescence rhythm initiates at a time corresponding to zeitgeber 12, independent of the time when the cells encysted. Phosphoprotein staining after two-dimensional polyacrylamide gel electrophoresis, as well as column-based phosphoprotein enrichment followed by liquid chromatography tandem mass spectrometry, showed cyst proteins are hypophosphorylated when compared with those from motile cells, with the most marked decreases found for predicted Casein Kinase2 target sites. In contrast to the phosphoproteome, the cyst proteome is not markedly different from motile cells, as assessed by two-dimensional polyacrylamide gel electrophoresis. In addition to changes in the phosphoproteome, RNA sequencing revealed that cysts show a significant decrease in the levels of 132 RNAs. Of the 42 RNAs that were identified by sequence analysis, 21 correspond to plastid-encoded gene products and 11 to nuclear-encoded cell wall/plasma membrane components. Our data are consistent with a model in which the highly reduced metabolism in cysts is achieved primarily by alterations in the phosphoproteome. The stalling of the circadian rhythm suggests temporary cysts may provide an interesting model to address the circadian system of dinoflagellates.

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“…Similarly, we observed a marked accumulation of both wcor18 transcript and the corresponding protein product following 42-day cold stress suggesting a possible role of these genes in long-term adaptation processes. In this regard, it is worthwhile to mention that both these proteins (wrab17, wcor18), and their corresponding genes, were recently found to show high level in abundance after prolonged cold treatment also in cold-tolerant wheat varieties [41,42]. On the other hand, Monroy et al [43] observed that spring and winter wheat cultivars share the same initial rapid expression of cold-inducible genes, but that their transcriptional profiles diverge widely during cold acclimation.…”

Section: Proteins Encoded By Genes With Stress-related Cis-regulatorymentioning

confidence: 97%

Proteomic analysis of a spring wheat cultivar in response to prolonged cold stress

et al. 2011

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Cold represents one of the major abiotic factors influencing plant growth and development worldwide. We analysed the long-term responsiveness of an Iranian spring wheat (cv. Kohdasht) to cold from a proteomic point of view, in order to unravel the molecular mechanisms helping a cold-sensitive cultivar to survive exposure to suboptimal temperatures. Plants were grown at 20 or 4°C until entering the reproductive stage and a cross-comparison on the leaf proteomes was performed. Quantitative analyses on protein alterations occurring upon low-temperature exposure showed a reinforcement in ascorbate recycling (dehydroascorbate reductase, ascorbate peroxidase) and protein processing (proteasome subunit, cysteine proteinase), as well as the accumulation of the enzyme devoted to tetrapyrrole resynthesis (glutamate semialdehyde aminomutase). In contrast, among proteins down-regulated after cold stress, we could identify some key Krebs cycle enzymes (isocitrate dehydrogenase, malate dehydrogenase), together with many photosynthesis-related proteins (oxygen-evolving complex proteins, ATP synthase subunits, ferredoxin NADPH oxidoreductase and some Calvin cycle enzymes). Physiological and biochemical parameters (such as shoot apex dissection, chlorophyll, proline and sugar content determination) sustained proteomics findings allowing the present research to contribute to the current knowledge on these long-term responses, which may be crucial to stress adaptation under field conditions.

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The influence of temperature on plant development in a vernalization-requiring winter wheat: A 2-DE based proteomic investigation

Cited by 56 publications

References 61 publications

Physiology and proteomics research on the leaves of ancient Platycladus orientalis (L.) during winter

Physiology and proteomics research on the leaves of ancient Platycladus orientalis (L.) during winter

Cold-Induced Cysts of the Photosynthetic DinoflagellateLingulodinium polyedrumHave an Arrested Circadian Bioluminescence Rhythm and Lower Levels of Protein Phosphorylation

Proteomic analysis of a spring wheat cultivar in response to prolonged cold stress

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